scholarly journals Evidence for SARS-CoV-2 Spike Protein in the Urine of COVID-19 patients

Author(s):  
Santosh George ◽  
Anasuya Chattopadhyay Pal ◽  
Jacqueline Gagnon ◽  
Sushma Timalsina ◽  
Pallavi Singh ◽  
...  

ABSTRACTSARS-CoV-2 infection has so far affected over 42 million people worldwide, causing over 1.1 million deaths. With the large majority of SARS-CoV-2 infected individuals being asymptomatic, major concerns have been raised about possible long-term consequences of the infection. We developed an antigen capture assay to detect SARS-CoV-2 spike protein in urine samples from COVID-19 patients whose diagnosis was confirmed by PCR from nasopharyngeal swabs (NP-PCR+). The study used a collection of 233 urine samples from 132 participants from Yale New Haven Hospital and the Children’s Hospital of Philadelphia obtained during the pandemic (106 NP-PCR+ and 26 NP-PCR-) as well as a collection of 20 urine samples from 20 individuals collected before the pandemic. Our analysis identified 23 out of 91 (25%) NP-PCR+ adult participants with SARS-CoV-2 spike S1 protein in urine (Ur-S+). Interestingly, although all NP-PCR+ children were Ur-S-, 1 NP-PCR-child was found to be positive for spike protein in urine. Of the 23 Ur-S+ adults, only 1 individual showed detectable viral RNA in urine. Our analysis further showed that 24% and 21% of NP-PCR+ adults have high levels of albumin and cystatin C in urine, respectively. Among individuals with albuminuria (>0.3 mg/mg of creatinine) statistical correlation could be found between albumin and spike protein in urine. Together, our data showe that 1 of 4 of SARS-CoV-2 infected individuals develop renal abnormalities such as albuminuria. Awareness about the long-term impact of these findings is warranted.

Kidney360 ◽  
2021 ◽  
pp. 10.34067/KID.0002172021
Author(s):  
Santosh George ◽  
Anasuya Chattopadhyay Pal ◽  
Jacqueline Gagnon ◽  
Sushma Timalsina ◽  
Pallavi Singh ◽  
...  

Background: SARS-CoV-2 infection has so far affected over 133 million people worldwide, causing over 2.5 million deaths. With the large majority of SARS-CoV-2 infected individuals being asymptomatic, major concerns have been raised about possible long-term consequences of the infection. Methods: We developed an antigen capture assay to detect SARS-CoV-2 spike protein in urine samples from COVID-19 patients whose diagnosis was confirmed by PCR from nasopharyngeal swabs (NP-PCR+). The study used a collection of 233 urine samples from 132 participants from Yale New Haven Hospital and the Children's Hospital of Philadelphia obtained during the pandemic (106 NP-PCR+ and 26 NP-PCR-) as well as a collection of 20 urine samples from 20 individuals collected before the pandemic. Results: Our analysis identified 23 out of 91 (25%) NP-PCR+ adult participants with SARS-CoV-2 spike S1 protein in urine (Ur-S+). Interestingly, although all NP-PCR+ children were Ur- S-, 1 NP-PCR- child was found to be positive for spike protein in urine. Of the 23 Ur-S+ adults, only 1 individual showed detectable viral RNA in urine. Our analysis further showed that 24% and 21% of NP-PCR+ adults have high levels of albumin and cystatin C in urine, respectively. Among individuals with albuminuria (>0.3 mg/mg of creatinine) statistical correlation could be found between albumin and spike protein in urine. Conclusions: Together, our data showed that 1 of 4 of SARS-CoV-2 infected individuals develop renal abnormalities such as albuminuria. Awareness about the long-term impact of these findings is warranted.


Crisis ◽  
2015 ◽  
Vol 36 (3) ◽  
pp. 220-224 ◽  
Author(s):  
Steven Stack

Abstract. Background: There has been no systematic work on the short- or long-term impact of the installation of crisis phones on suicides from bridges. The present study addresses this issue. Method: Data refer to 219 suicides from 1954 through 2013 on the Skyway Bridge in St. Petersburg, Florida. Six crisis phones with signs were installed in July 1999. Results: In the first decade after installation, the phones were used by 27 suicidal persons and credited with preventing 26 or 2.6 suicides a year. However, the net suicide count increased from 48 in the 13 years before installation of phones to 106 the following 13 years or by 4.5 additional suicides/year (t =3.512, p < .001). Conclusion: Although the phones prevented some suicides, there was a net increase after installation. The findings are interpreted with reference to suggestion/contagion effects including the emergence of a controversial bridge suicide blog.


2009 ◽  
Author(s):  
Jenna L. Claes ◽  
Sean S. Hankins ◽  
J. K. Ford
Keyword(s):  

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 966-P
Author(s):  
ATSUSHI FUJIYA ◽  
TOSHIKI KIYOSE ◽  
TAIGA SHIBATA ◽  
HIROSHI SOBAJIMA

Author(s):  
Xun Yuan ◽  
Andreas Mitsis ◽  
Thomas Semple ◽  
Michael Rubens ◽  
Christoph A. Nienaber

2020 ◽  
Vol 3 (3) ◽  
Author(s):  
Jiayue Jiao

 Economic vitality is an important indicator of regional competitiveness. The demand for talents and the vitality of enterprises in different regions are obvious to all and have practical significance. Therefore, it is necessary to establish a survey data model and conduct in-depth study on improving regional economic vitality from the perspective of policy.Based on a variety of forecasting methods, this paper analyzes the short-term and long-term impact of economic policies in Northeast China, and finally puts forward the factors that affect the economic vitality of northeast policies. Finally, the paper puts forward the feasibility and targeted suggestions of strengthening regional economic vitality, obtaining long-term development and building a more competitive city in the new era. 


Author(s):  
V.B. Kondratiev

The COVID-19 pandemic has affected the commodity markets and mining industry around the world in different ways. Mining company’s operations have been hit by coronavirus outbreaks and government-mandated production stops. Demand for many commodities remains low. This paper examines the potential long-term impact of the COVID-19 pandemic on future commodity demand, mining prospects, as well as tactical and strategic steps by mining companies to overcome the current crisis quickly and effectively.


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