scholarly journals RT-qPCR assay for detection of British (B.1.1.7) and South Africa (B.1.351) variants of SARS-CoV-2

Author(s):  
Karin Yaniv ◽  
Eden Ozer ◽  
Noam Plotkin ◽  
Nikhil Suresh Bhandarkar ◽  
Ariel Kushmaro

AbstractLess than a year following the SARS-CoV-2 outbreak, variants of concern have emerged in the form of the British variant B.1.1.7 and the South Africa variant B.1.351. Due to their high infectivity and morbidity, it is crucial to quickly and effectively detect them. Current methods of detection are either time-consuming, expensive or indirect. Here, we report the development of a rapid, cost-effective and direct RT-qPCR method for detection of the two variants of concern. We developed and validate a detection system for the detection of the B.1.1.7 variant and another single detection set for the B.1.351 variant. The developed approach was characterized and tested on wastewater samples and illustrated that all primers and probes were sensitive and specific. The novel system presented here will allow proper response and pandemic containment with regard to these variants. In addition, it may provide a basis for developing tools for the detection of additional variants of concern.

2019 ◽  
Vol 19 (1) ◽  
pp. 121-124
Author(s):  
Sandy Henderson ◽  
Ulrike Beland ◽  
Dimitrios Vonofakos

On or around 9 January 2019, twenty-two Listening Posts were conducted in nineteen countries: Canada, Chile, Denmark, Faroe Islands, Finland, Germany (Frankfurt and Berlin), Hungary, India, Ireland, Israel, Italy (two in Milan and one in the South), Peru, Serbia, South Africa, Spain, Sweden, Taiwan, Turkey, and the UK. This report synthesises the reports of those Listening Posts and organises the data yielded by them into common themes and patterns.


2020 ◽  
Vol 35 (1) ◽  
Author(s):  
Andre Mangu

After several decades of apartheid rule, which denied human rights to the majority of the population on the ground of race and came to be regarded as a crime against humanity, South Africa adopted its first democratic Constitution in the early 1990s. The 1996 Constitution, which succeeded the 1993 interim Constitution, is considered one of the most progressive in the world. In its founding provisions, it states that South Africa is a democratic state founded on human dignity, the achievement of equality, the advancement of human rights and freedoms. The Constitution enshrines fundamental human rights in a justiciable Bill of Rights as a cornerstone of democracy. Unfortunately, in the eyes of a number of politicians, officials and lay-persons, the rights in the Bill of Rights accrue to South African citizens only. Xenophobia, which has been rampant since the end of apartheid, seems to support the idea that foreigners should not enjoy these rights. Foreign nationals have often been accused of posing a threat to South African citizens with regard to employment opportunities. In light of the South African legislation and jurisprudence, this article affirms the position of the South African labour law that foreign nationals are indeed protected by the Constitution and entitled to rights in the Bill of Rights, including the rights to work and fair labour practices.


2020 ◽  
Vol 8 (1) ◽  
Author(s):  
Khangelani Moyo

Drawing on field research and a survey of 150 Zimbabwean migrants in Johannesburg, this paper explores the dimensions of migrants’ transnational experiences in the urban space. I discuss the use of communication platforms such as WhatsApp and Facebook as well as other means such as telephone calls in fostering the embedding of transnational migrants within both the Johannesburg and the Zimbabwean socio-economic environments. I engage this migrant-embedding using Bourdieusian concepts of “transnational habitus” and “transnational social field,” which are migration specific variations of Bourdieu’s original concepts of “habitus” and “social field.” In deploying these Bourdieusian conceptual tools, I observe that the dynamics of South–South migration as observed in the Zimbabwean migrants are different to those in the South–North migration streams and it is important to move away from using the same lens in interpreting different realities. For Johannesburg-based migrants to operate within the socio-economic networks produced in South Africa and in Zimbabwe, they need to actively acquire a transnational habitus. I argue that migrants’ cultivation of networks in Johannesburg is instrumental, purposive, and geared towards achieving specific and immediate goals, and latently leads to the development and sustenance of flexible forms of permanency in the transnational urban space.


2020 ◽  
Vol 8 (2) ◽  
Author(s):  
Bernard Nolen Fortuin

With the institution of compulsory military service in South Africa in 1948 the National Party government effected a tool well shaped for the construction of hegemonic masculinities. Through this, and other structures like schools and families, white children were shaped into submissive abiding citizens. Due to the brutal nature of a militarised society, gender roles become strictly defined and perpetuated. As such, white men’s time served on the border also “toughened” them up and shaped them into hegemonic copies of each other, ready to enforce patriarchal and racist ideologies. In this article, I look at how the novel Moffie by André Carl van der Merwe (2006) illustrates hegemonic white masculinity in South Africa and how it has long been strictly regulated to perpetuate the well-being of the white family as representative of the capitalist state. I discuss the novel by looking at the ways in which the narrator is marked by service in the military, which functions as a socialising agent, but as importantly by the looming threat of the application of the term “moffie” to himself, by self or others.  


2019 ◽  
Vol 16 (2) ◽  
Author(s):  
Mokoko Piet Sebola ◽  
Malemela Angelinah Mamabolo

The purpose of this article is to evaluate the engagement of farm beneficiaries in South Africa in the governance of restituted farms through communal property associations. The South African government has already spent millions of rands on land restitution to correct the imbalance of the past with regard to farm ownership by the African communities. Various methods of farm management to benefit the African society have been proposed, however, with little recorded success. This article argues that the South African post-apartheid government was so overwhelmed by political victory in 1994 that they introduced ambitious land reform policies that were based on ideal thinking rather than on a pragmatic approach to the South African situation. We used qualitative research methods to argue that the engagement of farm beneficiaries in farm management and governance through communal property associations is failing dismally. We conclude that a revisit of the communal property associations model is required in order to strengthen the position of beneficiaries and promote access to land by African communities for future benefit.


2020 ◽  
Vol 72 (1-3) ◽  
Author(s):  
Lungisani Moyo

ABSTRACT This paper used qualitative methodology to explore the South African government communication and land expropriation without compensation and its effects on food security using Alice town located in the Eastern Cape Province South Africa as its case study. This was done to allow the participants to give their perceptions on the role of government communication on land expropriation without compensation and its effects on South African food security. In this paper, a total population of 30 comprising of 26 small scale farmers in rural Alice and 4 employees from the Department of Agriculture (Alice), Eastern Cape, South Africa were interviewed to get their perception and views on government communications and land expropriation without compensation and its effects on South African food security. The findings of this paper revealed that the agricultural sector plays a vital role in the South African economy hence there is a great need to speed up transformation in the sector.


2017 ◽  
Vol 1 (1) ◽  
pp. 117
Author(s):  
Jared McDonald

Dr Jared McDonald, of the Department of History at the University of the Free State (UFS) in South Africa, reviews As by fire: the end of the South African university, written by former UFS vice-chancellor Jonathan Jansen.    How to cite this book review: MCDONALD, Jared. Book review: Jansen, J. 2017. As by Fire: The End of the South African University. Cape Town: Tafelberg.. Scholarship of Teaching and Learning in the South, [S.l.], v. 1, n. 1, p. 117-119, Sep. 2017. Available at: <http://sotl-south-journal.net/?journal=sotls&page=article&op=view&path%5B%5D=18>. Date accessed: 12 Sep. 2017.   This work is licensed under the Creative Commons Attribution 4.0 International License.To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/


Water Policy ◽  
2005 ◽  
Vol 7 (6) ◽  
pp. 627-642 ◽  
Author(s):  
Edina Sinanovic ◽  
Sandi Mbatsha ◽  
Stephen Gundry ◽  
Jim Wright ◽  
Clas Rehnberg

The burden of water-related disease is closely related to both the socio-economic situation and public health issues like access to clean water, sanitation and hygiene services. Poverty eradication, through improved access to water and sanitation, is the South African government's major priority. This is partly achieved through subsidising the cost of water and sanitation provision to the poor in rural areas. Whilst the new policies have made a remarkable impact on improved access to water and sanitation services, a general problem since the new approach in 1994 has been the lack of integration of policies for water and sanitation and health. This paper analyses the policies concerning rural water supply and sanitation in South Africa. It considers the structure of institutions, the division of responsibilities and legislated and financial capacity of the South Africa's water sector. A more integrated approach for the policies aiming at water access, sanitation and health is needed. In addition, as the local government's capacity to implement different programmes is limited, a review of the financing system is necessary.


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