scholarly journals Aging impairs the essential contributions of non-glial progenitors to neurorepair in the dorsal telencephalon of the Killifish N. furzeri

2021 ◽  
Author(s):  
Jolien Van houcke ◽  
Valerie Mariën ◽  
Caroline Zandecki ◽  
Sophie Vanhunsel ◽  
Lieve Moons ◽  
...  
Keyword(s):  
List Type ◽  
Proliferation Capacity ◽  
Nothobranchius Furzeri ◽  
Glial Progenitors ◽  
Brain Regeneration ◽  
Vertebrate Model ◽  
Regeneration Response ◽  
Dorsal Telencephalon ◽  
Newborn Neurons ◽  
Glial Scarring

SummaryThe aging central nervous system (CNS) of mammals displays progressive limited regenerative abilities. Recovery after loss of neurons is extremely restricted in the aged brain. Many research models fall short in recapitulating mammalian aging hallmarks or have an impractically long lifespan. We established a traumatic brain injury model in the African turquoise killifish (Nothobranchius furzeri), a regeneration-competent vertebrate model that evolved to naturally age extremely fast. Stab-wound injury of the aged killifish dorsal telencephalon unveils an impaired and incomplete regeneration response when compared to young individuals. Remarkably, killifish brain regeneration is mainly supported by atypical non-glial progenitors, yet their proliferation capacity appears declined with age. We identified a high inflammatory response and glial scarring to also underlie the hampered generation of new neurons in aged fish. These primary results will pave the way for further research to unravel the factor age in relation to neurorepair, and to improve therapeutic strategies to restore the injured and/or diseased aged mammalian CNS.HighlightsAging impairs neurorepair in the killifish pallium at multiple stages of the regeneration processAtypical non-glial progenitors support the production of new neurons in the naive and injured dorsal palliumThe impaired regeneration capacity of aged killifish is characterized by a reduced reactive proliferation of these progenitors followed by a decreased generation of newborn neurons that in addition, fail to reach the injury siteExcessive inflammation and glial scarring surface as potential brakes on brain repair in the aged killifish pallium

Intracardial perfusion of the African turquoise killifish v1

2021 ◽  
Author(s):  
Valerie Mariën ◽  
Jolien Van houcke ◽  
Lutgarde Arckens
Keyword(s):  
Single Cell ◽  
Single Cell Sequencing ◽  
Tissue Morphology ◽  
Nothobranchius Furzeri ◽  
Glial Progenitors ◽  
Dorsal Telencephalon

This perfusion protocol is essential for preserving tissue morphology in order to perform good quality immunohistochemical stainings. Here, we show you how we perform our perfusions on the African turquoise killifish. This protocol was already used in the following publications: Aging impairs the essential contributions of non-glial progenitors to neurorepair in the dorsal telencephalon of the Killifish Nothobranchius furzeri - PubMed (nih.gov) Single-cell sequencing of the adult killifish (N. furzeri) brain identifies an atypical progenitor, glial and neuronal heterogeneity | bioRxiv

scholarly journals Aging impairs the essential contributions of non‐glial progenitors to neurorepair in the dorsal telencephalon of the Killifish Nothobranchius furzeri

Aging Cell ◽  
2021 ◽  
Vol 20 (9) ◽  
Author(s):  
Jolien Van houcke ◽  
Valerie Mariën ◽  
Caroline Zandecki ◽  
Sophie Vanhunsel ◽  
Lieve Moons ◽  
...  
Keyword(s):  
Nothobranchius Furzeri ◽  
Glial Progenitors ◽  
Dorsal Telencephalon

scholarly journals Regulation of Life Span by The Gut Microbiota in The Short-Lived African Turquoise Killifish

2017 ◽  
Author(s):  
Patrick Smith ◽  
David Willemsen ◽  
Miriam Popkes ◽  
Franziska Metge ◽  
Edson Gandiwa ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Gut Microbiota ◽  
Life Span ◽  
Middle Age ◽  
External Environment ◽  
Systemic Effects ◽  
Life Span Extension ◽  
Nothobranchius Furzeri ◽  
Vertebrate Model

ABSTRACTGut bacteria occupy the interface between the organism and the external environment, contributing to homeostasis and disease. Yet, the causal role of the gut microbiota during host aging is largely unexplored. Here, using the African turquoise killifish (Nothobranchius furzeri), a naturally short-lived vertebrate, we show that the gut microbiota plays a key role in modulating vertebrate life span. Recolonizing the gut of middle-age individuals with bacteria from young donors resulted in life span extension and delayed behavioral decline. This intervention prevented the decrease in microbial diversity associated with host aging and maintained a young-like gut bacterial community, characterized by overrepresentation of the key genera Exiguobacterium, Planococcus, Propionigenium and Psychrobacter. Our findings demonstrate that the natural microbial gut community of young individuals can causally induce long-lasting beneficial systemic effects that lead to life span extension in a vertebrate model.

scholarly journals Mapping of quantitative trait loci controlling lifespan in the short‐lived fish Nothobranchius furzeri – a new vertebrate model for age research

Aging Cell ◽  
2012 ◽  
Vol 11 (2) ◽  
pp. 252-261 ◽  
Author(s):  
Jeanette Kirschner ◽  
David Weber ◽  
Christina Neuschl ◽  
Andre Franke ◽  
Marco Böttger ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Quantitative Trait ◽  
Nothobranchius Furzeri ◽  
Trait Loci ◽  
Vertebrate Model

scholarly journals Regulation of life span by the gut microbiota in the short-lived African turquoise killifish

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Patrick Smith ◽  
David Willemsen ◽  
Miriam Popkes ◽  
Franziska Metge ◽  
Edson Gandiwa ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Gut Microbiota ◽  
Life Span ◽  
Middle Age ◽  
External Environment ◽  
Systemic Effects ◽  
Life Span Extension ◽  
Nothobranchius Furzeri ◽  
Vertebrate Model

Gut bacteria occupy the interface between the organism and the external environment, contributing to homeostasis and disease. Yet, the causal role of the gut microbiota during host aging is largely unexplored. Here, using the African turquoise killifish (Nothobranchius furzeri), a naturally short-lived vertebrate, we show that the gut microbiota plays a key role in modulating vertebrate life span. Recolonizing the gut of middle-age individuals with bacteria from young donors resulted in life span extension and delayed behavioral decline. This intervention prevented the decrease in microbial diversity associated with host aging and maintained a young-like gut bacterial community, characterized by overrepresentation of the key genera Exiguobacterium, Planococcus, Propionigenium and Psychrobacter. Our findings demonstrate that the natural microbial gut community of young individuals can causally induce long-lasting beneficial systemic effects that lead to life span extension in a vertebrate model.

scholarly journals High tandem repeat content in the genome of the short-lived annual fish Nothobranchius furzeri: a new vertebrate model for aging research

2009 ◽  
Vol 10 (2) ◽  
pp. R16 ◽  
Author(s):  
Kathrin Reichwald ◽  
Chris Lauber ◽  
Indrajit Nanda ◽  
Jeanette Kirschner ◽  
Nils Hartmann ◽  
...  
Keyword(s):  
Tandem Repeat ◽  
Annual Fish ◽  
Aging Research ◽  
Repeat Content ◽  
Nothobranchius Furzeri ◽  
Vertebrate Model

scholarly journals Nothobranchius furzeri, an 'instant' fish from an ephemeral habitat

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Martin Reichard ◽  
Matej Polačik
Keyword(s):  
Laboratory Animal ◽  
Model Organism ◽  
Active Phase ◽  
Harsh Environment ◽  
Developmental Arrest ◽  
Active Life ◽  
Nothobranchius Furzeri ◽  
Ephemeral Habitat ◽  
Vertebrate Model ◽  
The Ideal

The turquoise killifish, Nothobranchius furzeri, is a promising vertebrate model in ageing research and an emerging model organism in genomics, regenerative medicine, developmental biology and ecotoxicology. Its lifestyle is adapted to the ephemeral nature of shallow pools on the African savannah. Its rapid and short active life commences when rains fill the pool: fish hatch, grow rapidly and mature in as few as two weeks, and then reproduce daily until the pool dries out. Its embryos then become inactive, encased in the dry sediment and protected from the harsh environment until the rains return. This invertebrate-like life cycle (short active phase and long developmental arrest) combined with a vertebrate body plan provide the ideal attributes for a laboratory animal.

Book Reviews

Tempo ◽  
1995 ◽  
pp. 29-36
Keyword(s):  
List Type

Volume I of Messiaen's ‘Traite’, ‘Music and Color’, and organ recordings Christopher DingleRobert Craft's Stravinsky memoirs and recordings Rodney Lister

Open discussion

1982 ◽  
Vol 99 ◽  
pp. 605-613
Author(s):  
P. S. Conti
Keyword(s):  
Buenos Aires ◽  
Large Mass ◽  
List Type ◽  
Common Phenomenon ◽  
Open Discussion ◽  
The Stability ◽  
Ring Nebulae

Conti: One of the main conclusions of the Wolf-Rayet symposium in Buenos Aires was that Wolf-Rayet stars are evolutionary products of massive objects. Some questions:–Do hot helium-rich stars, that are not Wolf-Rayet stars, exist?–What about the stability of helium rich stars of large mass? We know a helium rich star of ∼40 MO. Has the stability something to do with the wind?–Ring nebulae and bubbles : this seems to be a much more common phenomenon than we thought of some years age.–What is the origin of the subtypes? This is important to find a possible matching of scenarios to subtypes.

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