scholarly journals Rapid, Affordable and Scalable SARS-CoV-2 Detection from Saliva

2021 ◽  
Author(s):  
Andrew Hayden ◽  
Marcy Kuentzel ◽  
Sridar V Chittur
Keyword(s):  
Point Of Care ◽  
Phenol Red ◽  
Ph Indicator ◽  
Internal Validation ◽  
Dual Reporter ◽  
Care Facilities ◽  
End Point Detection ◽  
Point Detection ◽  
Syto 9 ◽  
Validation Of Results

Here we present an inexpensive, rapid, and robust RT-LAMP based SARS-CoV-2 detection method that is easily scalable, enabling point of care facilities and clinical labs to determine results from patients' saliva directly in 30 minutes for less than $2 a sample. The method utilizes a novel combination of widely available reagents that can be prepared in bulk, plated and frozen and remain stable until samples are received. This innovation dramatically reduces preparation time, enabling high-throughput automation and testing with time to results (including setup) in less than one hour for 96 patient samples simultaneously when using a 384 well format. By utilizing a dual-reporter (phenol red pH indicator for end-point detection and SYTO-9 fluorescent dye for real-time), the assay also provides internal validation of results and redundancy in the event of an instrument malfunction.

scholarly journals Acid number, viscosity and end-point detection in a multiphase high temperature polymerisation process using an online miniaturised MEMS Fabry-Pérot interferometer

Talanta ◽  
2021 ◽  
Vol 224 ◽  
pp. 121735
Author(s):  
Claudio Avila ◽  
Christos Mantzaridis ◽  
Joan Ferré ◽  
Rodrigo Rocha de Oliveira ◽  
Uula Kantojärvi ◽  
...  
Keyword(s):  
High Temperature ◽  
Acid Number ◽  
End Point ◽  
Fabry Perot ◽  
End Point Detection ◽  
Point Detection

Fluoroimmunoassay for human choriomammotropin.

1979 ◽  
Vol 25 (6) ◽  
pp. 973-975 ◽  
Author(s):  
T Chard ◽  
A Sykes
Keyword(s):  
Equipment Design ◽  
Radiation Hazard ◽  
Human Origin ◽  
End Point ◽  
End Point Detection ◽  
Purified Antigen ◽  
Point Detection

Abstract We describe an immunoassay for human choriomammo-tropin by use of the fluorescein-labeled hormone (of human origin). The technique is generally similar to the radioimmunoassay for this material, but has the advantage of stability of tracer and avoidance of radiation hazard. However, the procedure requires approximately 50-fold more tracer than does the radioimmunoassay, and this would be a disadvantage with materials for which supplies of purified antigen are scarce. Furthermore, both within-assay variation (3.9%) and between-assay variation (7.8--7.9%) were less satisfactory than that of radioimmunoassay (1.5% and 2.2--3%, respectively). This is almost certainly the result of imprecision of end-point detection and could probably be corrected by further attention to equipment design.

AEROBIC END-POINT DETECTION IN A SEQUENCING BATCH REACTOR

2005 ◽  
Vol 38 (1) ◽  
pp. 115-120
Author(s):  
Hilario López García ◽  
Iván Machón González
Keyword(s):  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
End Point ◽  
End Point Detection ◽  
Point Detection

Optical end point detection of etched YBCO films

1992 ◽  
Vol 191 (3-4) ◽  
pp. 525-529 ◽  
Author(s):  
R. Dolata ◽  
M. Fischer ◽  
W. Jutzi
Keyword(s):  
Ybco Films ◽  
End Point ◽  
End Point Detection ◽  
Point Detection

Complexometric titration of submicromole amounts of thorium(IV) with photometric end-point detection

1976 ◽  
Vol 65 (6) ◽  
pp. 667-672 ◽  
Author(s):  
C. J. C. Pijpers ◽  
L. G. Decnop-Weever ◽  
G. den Boef ◽  
W. E. van der Linden
Keyword(s):  
Complexometric Titration ◽  
End Point ◽  
End Point Detection ◽  
Point Detection
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