Integrative RNA-Seq analysis of Capsicum annuum L.-Phytophthora capsici L. pathosystem reveals molecular cross-talk and activation of host defence response

Background: Chili pepper (Capsicum annuum L.) being one of an important member of the Solanaceae family, and its productivity is highly affected by the fungal pathogen Phytophthora capsici L. Other to CM-344, the unavailability of resistant landraces to all possible strains of P. capsici imposes a serious threat to its global production. This is because of our current understanding of the molecular mechanisms associated with the defence response in C. annuum-P. capsici pathosystem is limited. Therefore, the current study used RNA-seq technology to dissect the genes associated with defence response against P. capsici infection in two contrasting landraces, i.e. GojamMecha_9086 (Resistant) and Dabat_80045 (susceptible) exposed to P. capsici infection. Results: The transcriptomes from 4 leaf samples (RC, RI, SC and SI) of chili pepper resulted in a total of 1,18, 879 assembled transcripts (with a mean TL of 813.23bp and N50 of 1,277bp) along with 52,384 pooled unigenes with (mean UL of 1029.36 bp and N50 of 1,403bp). The enrichment analysis of the transcripts indicated 23 different KEGG pathways under five main categories. Further, 774 and 484 differentially expressed genes (DEGs) were obtained from RC vs. RI and SC vs. SI leaf samples, respectively. Of these, 57 DEGs were found to be associated with defence responses against P. capsici infection. The defence-related genes, such as LTPL, defensin J1-2-like, peroxidase 5-like, UGT, and GRP proteins-like, were more significantly upregulated in RC vs. RI. Furthermore, RT-qPCR analysis of six randomly selected genes validated the results of Illumina NextSeq500 sequencing results. Furthermore, a total of 58 TF families (bHLH most abundant) and 2,095 protein families (Protein kinase, PF00069, most abundant) were observed across all the samples with maximum hits in RI and SI samples. Conclusions: RNA-Seq analysis of chili peppers during P.capsici infection revealed differential regulation of genes associated with defence and signaling response with shared coordination of molecular function, cellular component and biological processing. The results presented here would enhance our present understanding of the defence response in chili pepper against P. capsici infection, which could be utilized by the molecular breeders to develop resistant chili genotypes. Keywords: Capsicum annuum, Phytophthora capsici, transcriptome sequence, differential gene expression

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Transcriptomics Analysis of Heat Stress-Induced Genes in Pepper (Capsicum annuum L.) Seedlings

Horticulturae ◽

10.3390/horticulturae7100339 ◽

2021 ◽

Vol 7 (10) ◽

pp. 339

Author(s):

Fei Wang ◽

Yanxu Yin ◽

Chuying Yu ◽

Ning Li ◽

Sheng Shen ◽

...

Keyword(s):

Heat Stress ◽

Capsicum Annuum ◽

Molecular Mechanisms ◽

Enrichment Analysis ◽

Transcript Abundance ◽

Functional Enrichment Analysis ◽

Functional Enrichment ◽

Heat Shock Factors ◽

Subsequent Recovery ◽

Capsicum Annuum L

Pepper (Capsicum annuum L.) is one of the most economically important crops worldwide. Heat stress (HS) can significantly reduce pepper yield and quality. However, changes at a molecular level in response to HS and the subsequent recovery are poorly understood. In this study, 17-03 and H1023 were identified as heat-tolerant and heat-sensitive varieties, respectively. Their leaves’ transcript abundance was quantified using RNA sequencing to elucidate the effect of HS and subsequent recovery on gene expression. A total of 11,633 differentially expressed genes (DEGs) were identified, and the differential expression of 14 randomly selected DEGs was validated using reverse-transcription polymerase chain reaction. Functional enrichment analysis revealed that the most enriched pathways were metabolic processes under stress and photosynthesis and light harvesting during HS and after recovery from HS. The most significantly enriched pathways of 17-03 and H1023 were the same under HS, but differed during recovery. Furthermore, we identified 38 heat shock factors (Hsps), 17 HS transcription factors (Hsfs) and 38 NAC (NAM, ATAF1/2, and CUC2), and 35 WRKY proteins that were responsive to HS or recovery. These findings facilitate a better understanding of the molecular mechanisms underlying HS and recovery in different pepper genotypes.

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Characterization of Anthocyanins from Fruits of Two Mexican Chili Peppers (Capsicum annuum L.)

Journal of the Mexican Chemical Society ◽

10.29356/jmcs.v56i2.313 ◽

2017 ◽

Vol 56 (2) ◽

Cited By ~ 2

Author(s):

César Aza-González ◽

N. Ochoa-Alejo

Keyword(s):

Capsicum Annuum ◽

Chili Pepper ◽

Capsicum Annuum L ◽

Chili Peppers

Chili pepper fruits of the Árbol and Uvilla types (Capsicum annuum L.) were analyzed to characterize the anthocyanins that are synthesized and accumulated in the pericarp tissues. The aglycon delphinidin was identified as the only anthocyanidin in both chili peppers. Anthocyanins were separated and identified using HPLC-MS, and delphinidin-3-cis-coumaroylrutinoside-5-glucoside and delphinidin-3-trans-coumaroylrutinoside-5-glucoside were the most abundant components.

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