Phenotypic characterization of chili pepper (Capsicum annuum L.) under Phytophthora capsici infection and analysis of genetic diversity among identified resistance accessions using SSR markers

2020 ◽  
Vol 112 ◽  
pp. 101539 ◽  
Author(s):  
Tilahun Rabuma ◽  
Om Prakash Gupta ◽  
Vinod Chhokar
Keyword(s):  
Genetic Diversity ◽  
Capsicum Annuum ◽  
Ssr Markers ◽  
Phytophthora Capsici ◽  
Chili Pepper ◽  
Phenotypic Characterization ◽  
Capsicum Annuum L

Agro-morphological characterization of chili pepper landraces (Capsicum annuum L.) cultivated in Northern Benin

2017 ◽  
Vol 65 (2) ◽  
pp. 555-569 ◽  
Author(s):  
A. Orobiyi ◽  
L. Y. Loko ◽  
F. Sanoussi ◽  
A. P. Agré ◽  
N. Korie ◽  
...  
Keyword(s):  
Capsicum Annuum ◽  
Morphological Characterization ◽  
Chili Pepper ◽  
Capsicum Annuum L

Evaluation of Genetic Diversity of Red Pepper Landraces (Capsicum annuum L.) from Bulgaria Using SSR Markers

2012 ◽  
Vol 24 (5) ◽  
pp. 547-556 ◽  
Author(s):  
Se-Jong Oh ◽  
◽  
Jae-Young Song ◽  
Jeongran Lee ◽  
Gi-An Lee ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Capsicum Annuum ◽  
Ssr Markers ◽  
Red Pepper ◽  
Capsicum Annuum L

scholarly journals Characterization of Anthocyanins from Fruits of Two Mexican Chili Peppers (Capsicum annuum L.)

2017 ◽  
Vol 56 (2) ◽  
Author(s):  
César Aza-González ◽  
N. Ochoa-Alejo
Keyword(s):  
Capsicum Annuum ◽  
Chili Pepper ◽  
Capsicum Annuum L ◽  
Chili Peppers

Chili pepper fruits of the Árbol and Uvilla types (<em>Capsicum annuum L.</em>) were analyzed to characterize the anthocyanins that are synthesized and accumulated in the pericarp tissues. The aglycon delphinidin was identified as the only anthocyanidin in both chili peppers. Anthocyanins were separated and identified using HPLC-MS, and delphinidin-3-<em>cis</em>-coumaroylrutinoside-5-glucoside and delphinidin-3-<em>trans</em>-coumaroylrutinoside-5-glucoside were the most abundant components.

Genetic resources of chili pepper (Capsicum annuum L.) against Phytophthora capsici and their induction through various biotic and abiotic factors

2017 ◽  
Vol 51 (4) ◽  
pp. 296-304 ◽  
Author(s):  
Muhammad Umair Majid ◽  
Mudassar Fareed Awan ◽  
Kanza Fatima ◽  
Muhammad Sufyan Tahir ◽  
Qurban Ali ◽  
...  
Keyword(s):  
Genetic Resources ◽  
Capsicum Annuum ◽  
Abiotic Factors ◽  
Phytophthora Capsici ◽  
Chili Pepper ◽  
Biotic And Abiotic Factors ◽  
Capsicum Annuum L

scholarly journals Integrative RNA-Seq analysis of Capsicum annuum L.-Phytophthora capsici L. pathosystem reveals molecular cross-talk and activation of host defence response

2021 ◽  
Author(s):  
Tilahun Rabuma ◽  
Om Prakash Gupta ◽  
Vinod Chhokar X ◽  
Manju Yadav X
Keyword(s):  
Capsicum Annuum ◽  
Molecular Mechanisms ◽  
Phytophthora Capsici ◽  
Enrichment Analysis ◽  
Defence Response ◽  
Chili Pepper ◽  
Rna Seq ◽  
Kegg Pathways ◽  
Capsicum Annuum L ◽  
Chili Peppers

Background: Chili pepper (Capsicum annuum L.) being one of an important member of the Solanaceae family, and its productivity is highly affected by the fungal pathogen Phytophthora capsici L. Other to CM-344, the unavailability of resistant landraces to all possible strains of P. capsici imposes a serious threat to its global production. This is because of our current understanding of the molecular mechanisms associated with the defence response in C. annuum-P. capsici pathosystem is limited. Therefore, the current study used RNA-seq technology to dissect the genes associated with defence response against P. capsici infection in two contrasting landraces, i.e. GojamMecha_9086 (Resistant) and Dabat_80045 (susceptible) exposed to P. capsici infection. Results: The transcriptomes from 4 leaf samples (RC, RI, SC and SI) of chili pepper resulted in a total of 1,18, 879 assembled transcripts (with a mean TL of 813.23bp and N50 of 1,277bp) along with 52,384 pooled unigenes with (mean UL of 1029.36 bp and N50 of 1,403bp). The enrichment analysis of the transcripts indicated 23 different KEGG pathways under five main categories. Further, 774 and 484 differentially expressed genes (DEGs) were obtained from RC vs. RI and SC vs. SI leaf samples, respectively. Of these, 57 DEGs were found to be associated with defence responses against P. capsici infection. The defence-related genes, such as LTPL, defensin J1-2-like, peroxidase 5-like, UGT, and GRP proteins-like, were more significantly upregulated in RC vs. RI. Furthermore, RT-qPCR analysis of six randomly selected genes validated the results of Illumina NextSeq500 sequencing results. Furthermore, a total of 58 TF families (bHLH most abundant) and 2,095 protein families (Protein kinase, PF00069, most abundant) were observed across all the samples with maximum hits in RI and SI samples. Conclusions: RNA-Seq analysis of chili peppers during P.capsici infection revealed differential regulation of genes associated with defence and signaling response with shared coordination of molecular function, cellular component and biological processing. The results presented here would enhance our present understanding of the defence response in chili pepper against P. capsici infection, which could be utilized by the molecular breeders to develop resistant chili genotypes. Keywords: Capsicum annuum, Phytophthora capsici, transcriptome sequence, differential gene expression

scholarly journals Genetic Diversity Analysis of 41 Chili Pepper Genotypes [Capsicum annuum L.] Based on SSR Markers

2019 ◽  
Vol 15 (2) ◽  
pp. 65 ◽  
Author(s):  
Wartono Wartono ◽  
Suryo Wiyono ◽  
Muhamad Syukur ◽  
Giyanto Giyanto ◽  
Kristianto Nugroho ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Capsicum Annuum ◽  
Ssr Markers ◽  
Polyacrylamide Gel Electrophoresis ◽  
High Genetic Diversity ◽  
Genetic Studies ◽  
High Productivity ◽  
Capsicum Annuum L ◽  
Cultivar Development ◽  
Genetic Clusters

<p>Chili (Capsicum annuum L.) is an important horticultural crop which possesses high genetic diversity. Their genetic diversity needs to be assessed through molecular marker-based analysis to facilitate chili breeding scheme. The objective of the study was to analyze the genetic diversity of 41 chili genotypes using Simple Sequence Repeat (SSR) markers. Eleven SSR markers were used to amplify all genotypes through PCR technique, visualized on Polyacrylamide Gel Electrophoresis (PAGE). The data were analyzed using NTSYSpc version 2.1 and PowerMarker version 3.25 softwares. Results showed that the average of<br />SSR alleles was 7.9 with a range of 4-11 alleles per marker. All SSR markers showed PIC value &gt;0.5, indicating their suitable use for chili genetic studies. Cluster analysis of 41 genotypes generated three main groups according to coefficient similarity of 77.4%. The genetic clusters appeared as reflection of the genetic background of each genotype. In addition, the SSR markers used can identify potential parent as indicated by the genetic distance between genotypes. The highest genetic diversity (0.698) was demonstrated by combinations of Yuni × Ayesha, Yuni × Ayesha 2, and Yuni × Namira. The estimated genetic diversity produced by SSR markers in this study should be useful as guidance for chili breeders to select suitable parents for new cultivar development for high productivity and resistance to diseases.</p>

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