An immuno-enrichment free, validated quantification of tau protein in human CSF by LC-MS/MS
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Aims: Tau protein is a key target of interest in developing therapeutics for neurodegenerative diseases. Here, we sought to develop a method that quantifies extracellular tau protein concentrations human cerebrospinal fluid (CSF) without antibody-based enrichment strategies. Results: We demonstrate that the fit-for-purpose validated method in Alzheimers Disease CSF is limited to quasi quantitative measures of tau surrogate peptides. We also provide evidence that CSF total Tau measures by LC-MS are feasible in the presence of monoclonal therapeutic antibodies in human CSF. Conclusion: Our Tau LC-MS/MS method is a translational bioanalytical tool for assaying target
2011 ◽
Vol 17
(9)
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pp. 714-715
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2007 ◽
Vol 24
(3)
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pp. 207-212
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2001 ◽
Vol 297
(3)
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pp. 187-190
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1996 ◽
Vol 60
(2)
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pp. 237-238
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2019 ◽
Vol 72
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pp. 30-38
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