scholarly journals Sex Identification in Cattle, based on Amelogenin Gene

2021 ◽  
Author(s):  
Aftab Ahmad ◽  
Muhammad Israr ◽  
Murad Ali Rahat ◽  
Adnan Wahab ◽  
Subhan Uddin ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Base Pair ◽  
Sex Identification ◽  
Polymerase Chain Reaction Test ◽  
Chain Reaction ◽  
Reliable Technique ◽  
Amelogenin Gene ◽  
Base Pair Fragment ◽  
Polymerase Chain ◽  
Pair Fragment

Sex identification is considered an important step in forensic sciences, wildlife and livestock breeding management. In the current experiment we used Amelogenin gene as a biological marker for polymerase chain reaction test to identify the sex of cattle from blood remnants, collected at slaughter house. Due to the conserved region of the gene on both sex chromosomes (X and Y) a single primers pair was employed to amplify the gene in a single polymerase chain reaction. In case of band patterns, a 178 base pair fragment for AMELY and a 241 base pair fragment for AMELX genes were produced. The primer competence and exactness was initially checked on known gender cattle samples and then applied to unknown cattle samples for the validation of the experiment. PCR amplicons of unknown gender showed only one band (241-bp) for female DNA and two bands (241-bp, 178-bp) for male DNA, on the platform of agarose gel upon electrophoresis. Our findings showed that the PCR protocol based on AMELX or Y gene is a reliable technique for the identification of cattle sex.

scholarly journals Cytomegalovirus is not specifically associated with immunoglobulin A nephropathy.

1994 ◽  
Vol 4 (8) ◽  
pp. 1623-1626
Author(s):  
J S Park ◽  
J H Song ◽  
W S Yang ◽  
S B Kim ◽  
Y K Kim ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Immunoglobulin A ◽  
Renal Tissue ◽  
Early Gene ◽  
Chain Reaction ◽  
Indirect Immunofluorescence Staining ◽  
Base Pair Fragment ◽  
Polymerase Chain ◽  
Pair Fragment ◽  
Frequent Observation

Cytomegalovirus (CMV) has been suspected to be involved in the pathogenesis of IgA nephropathy (IgAN). Whether CMV is present in renal tissue of IgAN, however, remains controversial. To determine the presence of CMV in IgAN, compared with other glomerulonephritis (GN) as disease control, polymerase chain reaction amplifying a 159-base-pair fragment of the immediate early gene of CMV and indirect immunofluorescence staining with anti-CMV monoclonal antibody were performed on 10 IgAN and 14 non-IgAN GN renal tissues. CMV DNA was detected in 6 of 10 IgAN tissues and 10 of 14 other GN by polymerase chain reaction, whereas no CMV antigen was detected in all renal tissues by immunofluorescence. This frequent observation of CMV DNA in various types of GN as well as in IgAN would suggest that CMV is not specifically associated with the pathogenesis of IgAN seen in endemic areas of CMV infection.

Partial sequence of the 24S rRNA and polymerase chain reaction based assay for the toxic dinoflagellateDinophysis acuminata

1998 ◽  
Vol 55 (3) ◽  
pp. 597-604 ◽  
Author(s):  
O Puel ◽  
F Galgani ◽  
C Dalet ◽  
P Lassus
Keyword(s):  
Polymerase Chain Reaction ◽  
Pcr Amplification ◽  
Chain Reaction ◽  
Pcr Product ◽  
Dinophysis Acuminata ◽  
Base Pair Fragment ◽  
Variable Domains ◽  
Polymerase Chain ◽  
Seawater Samples ◽  
Pair Fragment

We describe the polymerase chain reaction (PCR) amplification of an 805 base pair fragment of 24S rRNA from the toxic dinoflagellate Dinophysis acuminata and the sequence of this fragment. We also describe a PCR-based assay for the specific detection of D. acuminata in seawater samples. Conserved primers, starting at positions 711 and 1489 of the 24S rRNA from the dinoflagellate Prorocentrum micans, were used for the PCR. The PCR product was cloned and sequenced. The fragment was aligned with rRNA sequences from other protists. Two oligonucleotides in variable domains of the sequence from D. acuminata were chosen and a protocol was defined for PCR-based detection of D. acuminata (30 cycles, 50°C). Experiments conducted with seawater samples led to the detection of D. acuminata in naturally contaminated samples. The PCR enabled us to detect down to 30 cells/L seawater. The problem of interference from large concentrations of other phytoplankton species may be solved using nested PCR.

Detection of the Glanzmann's Thrombasthenia Mutations in Arab and Iraqi-Jewish Patients by Polymerase Chain Reaction and Restriction Analysis of Blood or Urine Samples

1991 ◽  
Vol 66 (04) ◽  
pp. 500-504 ◽  
Author(s):  
H Peretz ◽  
U Seligsohn ◽  
E Zwang ◽  
B S Coller ◽  
P J Newman
Keyword(s):  
Polymerase Chain Reaction ◽  
Base Pair ◽  
Restriction Analysis ◽  
Urine Samples ◽  
Chain Reaction ◽  
Base Pairs ◽  
Glanzmann’S Thrombasthenia ◽  
Glanzmann's Thrombasthenia ◽  
Base Pair Deletion ◽  
Polymerase Chain

SummarySevere Glanzmann's thrombasthenia is relatively frequent in Iraqi-Jews and Arabs residing in Israel. We have recently described the mutations responsible for the disease in Iraqi-Jews – an 11 base pair deletion in exon 12 of the glycoprotein IIIa gene, and in Arabs – a 13 base pair deletion at the AG acceptor splice site of exon 4 on the glycoprotein IIb gene. In this communication we show that the Iraqi-Jewish mutation can be identified directly by polymerase chain reaction and gel electrophoresis. With specially designed oligonucleotide primers encompassing the mutation site, an 80 base pair segment amplified in healthy controls was clearly distinguished from the 69 base pair segment produced in patients. Patients from 11 unrelated Iraqi-Jewish families had the same mutation. The Arab mutation was identified by first amplifying a DNA segment consisting of 312 base pairs in controls and of 299 base pairs in patients, and then digestion by a restriction enzyme Stu-1, which recognizes a site that is absent in the mutant gene. In controls the 312 bp segment was digested into 235 and 77 bp fragments, while in patients there was no change in the size of the amplified 299 bp segment. The mutation was found in patients from 3 out of 5 unrelated Arab families. Both Iraqi-Jewish and Arab mutations were detectable in DNA extracted from blood and urine samples. The described simple methods of identifying the mutations should be useful for detection of the numerous potential carriers among the affected kindreds and for prenatal diagnosis using DNA extracted from chorionic villi samples.

scholarly journals EXPRESS: Clinical performance of the Elecsys® Anti-SARS-CoV-2 combined in an algorithm with two specific anti-IgG immunoassays for the evaluation of the serological response of patients with COVID-19 in a population with a high prevalence of infection

2021 ◽  
pp. 000456322110380
Author(s):  
Xavier Gabaldó-Barrios ◽  
Simona Iftimie ◽  
Anna Hernández-Aguilera ◽  
Isabel Pujol ◽  
Frederic Ballester ◽  
...  
Keyword(s):  
Immune Response ◽  
Polymerase Chain Reaction ◽  
Predictive Value ◽  
Clinical Performance ◽  
Polymerase Chain Reaction Test ◽  
Serological Response ◽  
Chain Reaction ◽  
Automated Assay ◽  
High Prevalence ◽  
Polymerase Chain

Background: Anti-SARS-CoV-2 antibodies have been used in the study of the immune response in infected patients. However, differences in sensitivity and specificity have been reported, depending on the method of analysis. The aim of the present study was to evaluate the diagnostic accuracy of an algorithm in which a high-throughput automated assay for total antibodies was used for screening and two semi-automated IgG-specific methods were used to confirm the results, and also to correlate the analytical results with the clinical data and the time elapsed since infection. Methods: We studied 306 patients, some hospitalized and some outpatients, belonging to a population with a high prevalence of COVID-19. One-hundred and ten patients were classified as SARS-CoV-2 negative and 196 as positive by polymerase chain reaction. Results: The algorithm and automated assay alone had a specificity and a positive predictive value of 100%, although the sensitivity and negative predictive value of the algorithm was higher. Both methods showed a good sensitivity from day 11 of the onset of symptoms in asymptomatic and symptomatic patients. The absorbance of the total antibodies was significantly higher in severely symptomatic than in asymptomatic or mildly symptomatic patients, which suggests the antibody level was higher. We found 15 patients that did not present seroconversion at 12 days from the onset of symptoms or the first polymerase chain reaction test. Conclusion: This study highlights the proper functioning of algorithms in the diagnosis of the immune response to COVID-19, which can help to define testing strategies against this disease.

scholarly journals Clinical Evaluation of a 16S Ribosomal RNA Polymerase Chain Reaction Test for the Diagnosis of Lymph Node Tuberculosis

2006 ◽  
Vol 43 (7) ◽  
pp. 855-859 ◽  
Author(s):  
Fernando Osores ◽  
Oscar Nolasco ◽  
Kristien Verdonck ◽  
Jorge Arevalo ◽  
Juan Carlos Ferrufino ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Lymph Node ◽  
Rna Polymerase ◽  
Ribosomal Rna ◽  
Polymerase Chain Reaction Test ◽  
Chain Reaction ◽  
Reaction Test ◽  
Polymerase Chain ◽  
Lymph Node Tuberculosis ◽  
Chain Reaction Test
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