scholarly journals CarboGrove: a resource of glycan-binding specificities through analyzed glycan-array datasets from all platforms

2021 ◽  
Author(s):  
Zachary L Klamer ◽  
Chelsea M Harris ◽  
Jonathan M Beirne ◽  
Jessica E Kelly ◽  
Jian Zhang ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Experimental Methods ◽  
Common Method ◽  
Glycan Array ◽  
Array Data ◽  
Meta Analyses

The volume and value of glycan-array data are increasing, but no common method and resource exists to analyze, integrate, and use the available data. To meet this need, we developed a resource of analyzed glycan-array data called CarboGrove. Building on the ability to process and interpret data from any type of glycan array, we populated the database with the results from 35 types of glycan arrays, 13 glycan families, 5 experimental methods, and 19 laboratories or companies. In meta-analyses of glycan-binding proteins, we observed glycan-binding specificities that were not uncovered from single sources. In addition, we confirmed the ability to efficiently optimize selections of glycan-binding proteins to be used in experiments for discriminating between closely related motifs. CarboGrove yields unprecedented access to the wealth of glycan-array data being produced and powerful capabilities for both experimentalists and bioinformaticians.

scholarly journals Exploring the Specificities of Glycan-Binding Proteins Using Glycan Array Data and the GlycoSearch Software

2015 ◽  
pp. 203-214 ◽  
Author(s):  
Doron Kletter ◽  
Bryan Curnutte ◽  
Kevin A. Maupin ◽  
Marshall Bern ◽  
Brian B. Haab
Keyword(s):  
Binding Proteins ◽  
Glycan Array ◽  
Array Data

scholarly journals A motif-based analysis of glycan array data to determine the specificities of glycan-binding proteins

Glycobiology ◽  
2009 ◽  
Vol 20 (3) ◽  
pp. 369-380 ◽  
Author(s):  
Andrew Porter ◽  
Tingting Yue ◽  
Lee Heeringa ◽  
Steven Day ◽  
Edward Suh ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Glycan Array ◽  
Array Data

scholarly journals Microbe-focused glycan array screening platform

2019 ◽  
Vol 116 (6) ◽  
pp. 1958-1967 ◽  
Author(s):  
Andreas Geissner ◽  
Anika Reinhardt ◽  
Christoph Rademacher ◽  
Timo Johannssen ◽  
João Monteiro ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Microarray Platform ◽  
Innate Immune ◽  
Glycan Array ◽  
Phase Synthesis ◽  
Glycan Microarray ◽  
Solution Phase Synthesis ◽  
Screening Platform ◽  
Excellent Tool

Interactions between glycans and glycan binding proteins are essential for numerous processes in all kingdoms of life. Glycan microarrays are an excellent tool to examine protein–glycan interactions. Here, we present a microbe-focused glycan microarray platform based on oligosaccharides obtained by chemical synthesis. Glycans were generated by combining different carbohydrate synthesis approaches including automated glycan assembly, solution-phase synthesis, and chemoenzymatic methods. The current library of more than 300 glycans is as diverse as the mammalian glycan array from the Consortium for Functional Glycomics and, due to its microbial focus, highly complementary. This glycan platform is essential for the characterization of various classes of glycan binding proteins. Applications of this glycan array platform are highlighted by the characterization of innate immune receptors and bacterial virulence factors as well as the analysis of human humoral immunity to pathogenic glycans.

scholarly journals Printed covalent glycan array for ligand profiling of diverse glycan binding proteins

2004 ◽  
Vol 101 (49) ◽  
pp. 17033-17038 ◽  
Author(s):  
O. Blixt ◽  
S. Head ◽  
T. Mondala ◽  
C. Scanlan ◽  
M. E. Huflejt ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Glycan Array

scholarly journals Combining mass spectrometry and glycan array data to explore invertebrate glycomes

2018 ◽  
Vol 32 (S1) ◽  
Author(s):  
Iain Wilson ◽  
Barbara Eckmair ◽  
Alba Hykollari ◽  
Shi Yan ◽  
Jorick Vanbeselaere ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Glycan Array ◽  
Array Data

scholarly journals Determining Lectin Specificity from Glycan Array Data Using Motif Segregation and GlycoSearch Software

2013 ◽  
Vol 5 (2) ◽  
pp. 157-169 ◽  
Author(s):  
Doron Kletter ◽  
Zheng Cao ◽  
Marshall Bern ◽  
Brian Haab
Keyword(s):  
Glycan Array ◽  
Array Data ◽  
Lectin Specificity

scholarly journals Global Comparisons of Lectin–Glycan Interactions Using a Database of Analyzed Glycan Array Data

2013 ◽  
Vol 12 (4) ◽  
pp. 1026-1035 ◽  
Author(s):  
Doron Kletter ◽  
Sudhir Singh ◽  
Marshall Bern ◽  
Brian B. Haab
Keyword(s):  
Glycan Array ◽  
Array Data
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