Microbe-focused glycan array screening platform

Interactions between glycans and glycan binding proteins are essential for numerous processes in all kingdoms of life. Glycan microarrays are an excellent tool to examine protein–glycan interactions. Here, we present a microbe-focused glycan microarray platform based on oligosaccharides obtained by chemical synthesis. Glycans were generated by combining different carbohydrate synthesis approaches including automated glycan assembly, solution-phase synthesis, and chemoenzymatic methods. The current library of more than 300 glycans is as diverse as the mammalian glycan array from the Consortium for Functional Glycomics and, due to its microbial focus, highly complementary. This glycan platform is essential for the characterization of various classes of glycan binding proteins. Applications of this glycan array platform are highlighted by the characterization of innate immune receptors and bacterial virulence factors as well as the analysis of human humoral immunity to pathogenic glycans.

Download Full-text

Solution-Phase Synthesis and Characterization of Single-Crystalline SnSe Nanowires

Angewandte Chemie ◽

10.1002/ange.201105614 ◽

2011 ◽

Vol 123 (50) ◽

pp. 12256-12259 ◽

Cited By ~ 10

Author(s):

Sheng Liu ◽

Xiaoyang Guo ◽

Mingrun Li ◽

Wen-Hua Zhang ◽

Xingyuan Liu ◽

...

Keyword(s):

Solution Phase ◽

Synthesis And Characterization ◽

Phase Synthesis ◽

Single Crystalline ◽

Solution Phase Synthesis

Download Full-text

Solution-Phase Synthesis and Characterization of Perovskite LaCoO3 Nanocrystals via A Co-Precipitation Route

Journal of Rare Earths ◽

10.1016/s1002-0721(07)60570-5 ◽

2007 ◽

Vol 25 (5) ◽

pp. 601-604 ◽

Cited By ~ 26

Author(s):

Zhu Junwu ◽

Sun Xiaojie ◽

Wang Yanping ◽

Wang Xin ◽

Yang Xujie ◽

...

Keyword(s):

Solution Phase ◽

Synthesis And Characterization ◽

Phase Synthesis ◽

Solution Phase Synthesis ◽

Co Precipitation

Download Full-text

Rapid Microwave-Assisted Solution Phase Synthesis and Spectroscopic Characterization of Salicylidene-o-Aminophenol and Its Metal(II) Complexes

Synthesis and Reactivity in Inorganic Metal-Organic and Nano-Metal Chemistry ◽

10.1080/15533170802265749 ◽

2008 ◽

Vol 38 (6) ◽

pp. 550-552 ◽

Cited By ~ 2

Author(s):

Jian Gao ◽

Tong-Tao Xu

Keyword(s):

Spectroscopic Characterization ◽

Solution Phase ◽

Phase Synthesis ◽

Microwave Assisted ◽

Solution Phase Synthesis

Download Full-text

Synthesis and structural characterization of monomeric and dimeric peptide nucleic acids prepared by using microwave-promoted multicomponent reactions

Organic & Biomolecular Chemistry ◽

10.1039/c5ob01604e ◽

2015 ◽

Vol 13 (45) ◽

pp. 11052-11071 ◽

Cited By ~ 2

Author(s):

Reuben Ovadia ◽

Aurélien Lebrun ◽

Ivan Barvik ◽

Jean-Jacques Vasseur ◽

Carine Baraguey ◽

...

Keyword(s):

Nucleic Acid ◽

Nucleic Acids ◽

Structural Characterization ◽

Peptide Nucleic Acid ◽

Multicomponent Reactions ◽

Peptide Nucleic Acids ◽

Solution Phase ◽

Phase Synthesis ◽

Solution Phase Synthesis

A solution phase synthesis of peptide nucleic acid monomers and dimers was developed by using microwave-promoted Ugi multicomponent reactions.

Download Full-text

Ticks produce highly selective chemokine binding proteins with antiinflammatory activity

Journal of Experimental Medicine ◽

10.1084/jem.20072689 ◽

2008 ◽

Vol 205 (9) ◽

pp. 2019-2031 ◽

Cited By ~ 142

Author(s):

Maud Déruaz ◽

Achim Frauenschuh ◽

Ana L. Alessandri ◽

João M. Dias ◽

Fernanda M. Coelho ◽

...

Keyword(s):

Immune System ◽

Binding Proteins ◽

Tertiary Structure ◽

Innate Immune ◽

Expression Cloning ◽

Host Immune System ◽

Animal Models Of Disease ◽

Single Domain Antibodies ◽

Models Of Disease

Bloodsucking parasites such as ticks have evolved a wide variety of immunomodulatory proteins that are secreted in their saliva, allowing them to feed for long periods of time without being detected by the host immune system. One possible strategy used by ticks to evade the host immune response is to produce proteins that selectively bind and neutralize the chemokines that normally recruit cells of the innate immune system that protect the host from parasites. We have identified distinct cDNAs encoding novel chemokine binding proteins (CHPBs), which we have termed Evasins, using an expression cloning approach. These CHBPs have unusually stringent chemokine selectivity, differentiating them from broader spectrum viral CHBPs. Evasin-1 binds to CCL3, CCL4, and CCL18; Evasin-3 binds to CXCL8 and CXCL1; and Evasin-4 binds to CCL5 and CCL11. We report the characterization of Evasin-1 and -3, which are unrelated in primary sequence and tertiary structure, and reveal novel folds. Administration of recombinant Evasin-1 and -3 in animal models of disease demonstrates that they have potent antiinflammatory properties. These novel CHBPs designed by nature are even smaller than the recently described single-domain antibodies (Hollinger, P., and P.J. Hudson. 2005. Nat. Biotechnol. 23:1126–1136), and may be therapeutically useful as novel antiinflammatory agents in the future.

Download Full-text