scholarly journals Printed covalent glycan array for ligand profiling of diverse glycan binding proteins

2004 ◽  
Vol 101 (49) ◽  
pp. 17033-17038 ◽  
Author(s):  
O. Blixt ◽  
S. Head ◽  
T. Mondala ◽  
C. Scanlan ◽  
M. E. Huflejt ◽  
...  
2019 ◽  
Vol 116 (6) ◽  
pp. 1958-1967 ◽  
Author(s):  
Andreas Geissner ◽  
Anika Reinhardt ◽  
Christoph Rademacher ◽  
Timo Johannssen ◽  
João Monteiro ◽  
...  

Interactions between glycans and glycan binding proteins are essential for numerous processes in all kingdoms of life. Glycan microarrays are an excellent tool to examine protein–glycan interactions. Here, we present a microbe-focused glycan microarray platform based on oligosaccharides obtained by chemical synthesis. Glycans were generated by combining different carbohydrate synthesis approaches including automated glycan assembly, solution-phase synthesis, and chemoenzymatic methods. The current library of more than 300 glycans is as diverse as the mammalian glycan array from the Consortium for Functional Glycomics and, due to its microbial focus, highly complementary. This glycan platform is essential for the characterization of various classes of glycan binding proteins. Applications of this glycan array platform are highlighted by the characterization of innate immune receptors and bacterial virulence factors as well as the analysis of human humoral immunity to pathogenic glycans.


2021 ◽  
Author(s):  
Zachary L Klamer ◽  
Chelsea M Harris ◽  
Jonathan M Beirne ◽  
Jessica E Kelly ◽  
Jian Zhang ◽  
...  

The volume and value of glycan-array data are increasing, but no common method and resource exists to analyze, integrate, and use the available data. To meet this need, we developed a resource of analyzed glycan-array data called CarboGrove. Building on the ability to process and interpret data from any type of glycan array, we populated the database with the results from 35 types of glycan arrays, 13 glycan families, 5 experimental methods, and 19 laboratories or companies. In meta-analyses of glycan-binding proteins, we observed glycan-binding specificities that were not uncovered from single sources. In addition, we confirmed the ability to efficiently optimize selections of glycan-binding proteins to be used in experiments for discriminating between closely related motifs. CarboGrove yields unprecedented access to the wealth of glycan-array data being produced and powerful capabilities for both experimentalists and bioinformaticians.


Author(s):  
Doron Kletter ◽  
Bryan Curnutte ◽  
Kevin A. Maupin ◽  
Marshall Bern ◽  
Brian B. Haab

Glycobiology ◽  
2009 ◽  
Vol 20 (3) ◽  
pp. 369-380 ◽  
Author(s):  
Andrew Porter ◽  
Tingting Yue ◽  
Lee Heeringa ◽  
Steven Day ◽  
Edward Suh ◽  
...  

Author(s):  
J. Jakana ◽  
M.F. Schmid ◽  
P. Matsudaira ◽  
W. Chiu

Actin is a protein found in all eukaryotic cells. In its polymerized form, the cells use it for motility, cytokinesis and for cytoskeletal support. An example of this latter class is the actin bundle in the acrosomal process from the Limulus sperm. The different functions actin performs seem to arise from its interaction with the actin binding proteins. A 3-dimensional structure of this macromolecular assembly is essential to provide a structural basis for understanding this interaction in relationship to its development and functions.


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