scholarly journals A multiscale X-ray phase-contrast tomography dataset of whole human left lung

2021 ◽  
Author(s):  
R. Patrick Xian ◽  
Claire L. Walsh ◽  
Stijn E. Verleden ◽  
Willi L. Wagner ◽  
Alexandre Bellier ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Ex Vivo ◽  
Scale Up ◽  
Left Lung ◽  
Tissue Imaging ◽  
Fourth Generation ◽  
Voxel Size ◽  
Synchrotron Source ◽  
X Ray ◽  
Phase Contrast Tomography

Technological advancements in X-ray imaging using bright and coherent synchrotron sources now allows to decouple sample size and resolution, while maintaining high sensitivity to the microstructure of soft, partially dehydrated tissues. The recently developed imaging technique, hierarchical phase-contrast tomography, is a comprehensive approach to address the challenge of organ-scale (up to tens of centimeters) soft tissue imaging with resolution and sensitivity down to the cellular level. Using this technique, we imaged ex vivo an entire human left lung at an isotropic voxel size of 25.08 μm along with local zooms down to 6.05 - 6.5 μm and 2.45 - 2.5 μm in voxel size. The high tissue contrast offered by the fourth-generation synchrotron source at the European Synchrotron Radiation Facility reveals complex multiscale anatomical constitution of the human lung from the macroscopic (centimeter) down to the microscopic (micrometer) scale. The dataset provides complete organ-scale 3D information of the secondary pulmonary lobules and delineates the microstructure of lung nodules with unprecedented detail.

Quantitative characterization of ex vivo breast tissue via x-ray phase-contrast tomography

2019 ◽  
Vol 27 (3) ◽  
pp. 503-516
Author(s):  
Xinbin Li ◽  
Zhiqiang Chen ◽  
Li Zhang ◽  
Xiaohua Zhu ◽  
Shengping Wang ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Breast Tissue ◽  
Ex Vivo ◽  
Quantitative Characterization ◽  
X Ray ◽  
Phase Contrast Tomography

scholarly journals Compact laser accelerators for X-ray phase-contrast imaging

2014 ◽  
Vol 372 (2010) ◽  
pp. 20130032 ◽  
Author(s):  
Z. Najmudin ◽  
S. Kneip ◽  
M. S. Bloom ◽  
S. P. D. Mangles ◽  
O. Chekhlov ◽  
...  
Keyword(s):  
Generation X ◽  
Phase Contrast ◽  
Electron Beams ◽  
Imaging Techniques ◽  
Phase Contrast Imaging ◽  
Fourth Generation ◽  
Light Sources ◽  
Contrast Imaging ◽  
Synchrotron Source ◽  
X Ray

Advances in X-ray imaging techniques have been driven by advances in novel X-ray sources. The latest fourth-generation X-ray sources can boast large photon fluxes at unprecedented brightness. However, the large size of these facilities means that these sources are not available for everyday applications. With advances in laser plasma acceleration, electron beams can now be generated at energies comparable to those used in light sources, but in university-sized laboratories. By making use of the strong transverse focusing of plasma accelerators, bright sources of betatron radiation have been produced. Here, we demonstrate phase-contrast imaging of a biological sample for the first time by radiation generated by GeV electron beams produced by a laser accelerator. The work was performed using a greater than 300 TW laser, which allowed the energy of the synchrotron source to be extended to the 10–100 keV range.

scholarly journals X-ray phase contrast tomography for the investigation of amyotrophic lateral sclerosis

2020 ◽  
Vol 27 (4) ◽  
pp. 1042-1048 ◽  
Author(s):  
Ginevra Begani Provinciali ◽  
Nicola Pieroni ◽  
Inna Bukreeva ◽  
Michela Fratini ◽  
Lorenzo Massimi ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Amyotrophic Lateral Sclerosis ◽  
Phase Contrast ◽  
Neurodegenerative Disorder ◽  
Ex Vivo ◽  
Limiting Current ◽  
Early Assessment ◽  
X Ray ◽  
Phase Contrast Tomography ◽  
Lateral Sclerosis

Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disorder affecting motor neurons. Pre-clinical studies drive the development of animal models that well mimic ALS disorder and enable both the dissection of disease processes and an early assessment of therapy efficacy. A comprehensive knowledge of neuronal and vascular lesions in the brain and spinal cord is an essential factor to understand the development of the disease. Spatial resolution and bidimensional imaging are important drawbacks limiting current neuroimaging tools, while neuropathology relies on protocols that may alter tissue chemistry and structure. In contrast, recent ex vivo studies in mice demonstrated that X-ray phase-contrast tomography enables study of the 3D distribution of both vasculature and neuronal networks, without sample sectioning or use of staining. Here we present our findings on ex vivo SOD1G93A ALS mice spinal cord at a micrometric scale. An unprecedented direct quantification of neuro-vascular alterations at different stages of the disease is shown.

Investigation of Herculaneum Papyri by X-Ray Phase-Contrast Tomography

2019 ◽  
pp. 299-324
Author(s):  
Inna Bukreeva ◽  
Graziano Ranocchia ◽  
Vincenzo Formoso ◽  
Michele Alessandrelli ◽  
Michela Fratini ◽  
...  
Keyword(s):  
Phase Contrast ◽  
X Ray ◽  
Phase Contrast Tomography

scholarly journals Morphological Evolution of Electrochemically Plated/Stripped Lithium Microstructures Investigated by Synchrotron X-ray Phase Contrast Tomography

ACS Nano ◽  
2016 ◽  
Vol 10 (8) ◽  
pp. 7990-7997 ◽  
Author(s):  
Fu Sun ◽  
Lukas Zielke ◽  
Henning Markötter ◽  
André Hilger ◽  
Dong Zhou ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Morphological Evolution ◽  
X Ray ◽  
Phase Contrast Tomography

scholarly journals Assessment of intraductal carcinoma in situ (DCIS) using grating-based X-ray phase-contrast CT at conventional X-ray sources: An experimental ex-vivo study

PLoS ONE ◽  
2019 ◽  
Vol 14 (1) ◽  
pp. e0210291 ◽  
Author(s):  
Karin Hellerhoff ◽  
Lorenz Birnbacher ◽  
Anikó Sztrókay-Gaul ◽  
Susanne Grandl ◽  
Sigrid Auweter ◽  
...  
Keyword(s):  
Carcinoma In Situ ◽  
Phase Contrast ◽  
Ex Vivo ◽  
Intraductal Carcinoma ◽  
X Ray ◽  
Contrast Ct ◽  
Ex Vivo Study

scholarly journals Three-dimensional virtual histology of human cerebellum by X-ray phase-contrast tomography

2018 ◽  
Vol 115 (27) ◽  
pp. 6940-6945 ◽  
Author(s):  
Mareike Töpperwien ◽  
Franziska van der Meer ◽  
Christine Stadelmann ◽  
Tim Salditt
Keyword(s):  
Phase Contrast ◽  
Spatial Organization ◽  
Granular Layer ◽  
Three Dimensional ◽  
Cell Segmentation ◽  
X Ray ◽  
Human Cerebellum ◽  
Conventional Histology ◽  
3D Information ◽  
Phase Contrast Tomography

To quantitatively evaluate brain tissue and its corresponding function, knowledge of the 3D cellular distribution is essential. The gold standard to obtain this information is histology, a destructive and labor-intensive technique where the specimen is sliced and examined under a light microscope, providing 3D information at nonisotropic resolution. To overcome the limitations of conventional histology, we use phase-contrast X-ray tomography with optimized optics, reconstruction, and image analysis, both at a dedicated synchrotron radiation endstation, which we have equipped with X-ray waveguide optics for coherence and wavefront filtering, and at a compact laboratory source. As a proof-of-concept demonstration we probe the 3D cytoarchitecture in millimeter-sized punches of unstained human cerebellum embedded in paraffin and show that isotropic subcellular resolution can be reached at both setups throughout the specimen. To enable a quantitative analysis of the reconstructed data, we demonstrate automatic cell segmentation and localization of over 1 million neurons within the cerebellar cortex. This allows for the analysis of the spatial organization and correlation of cells in all dimensions by borrowing concepts from condensed-matter physics, indicating a strong short-range order and local clustering of the cells in the granular layer. By quantification of 3D neuronal “packing,” we can hence shed light on how the human cerebellum accommodates 80% of the total neurons in the brain in only 10% of its volume. In addition, we show that the distribution of neighboring neurons in the granular layer is anisotropic with respect to the Purkinje cell dendrites.

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