Oxycodone decreases anxiety-like behavior in the elevated plus-maze test in male and female rats

AbstractThe prescription opioid oxycodone is widely used for the treatment of pain in humans. Oxycodone misuse is more common among people with an anxiety disorder than those without one. Therefore, oxycodone might be misused for its anxiolytic properties. We investigated if oxycodone affects anxiety-like behavior in adult male and female rats. The rats were treated with oxycodone (0.178, 0.32, 0.56, or 1 mg/kg), and anxiety-like behavior was investigated in the elevated plus-maze test. Immediately after the elevated plus-maze test, a small open field test was conducted to determine the effects of oxycodone on locomotor activity. In the elevated plus-maze test, oxycodone increased the percentage of time spent on the open arms, the percentage of open arm entries, time on the open arms, open arm entries, and the distance traveled. The males treated with vehicle had a lower percentage of open arm entries than the females treated with vehicle, and oxycodone treatment led to a greater increase in the percentage of open arm entries in the males than females. Furthermore, the females spent more time on the open arms, made more open arm entries, spent less time in the closed arms, and traveled a greater distance than the males. In the small open field test, treatment with oxycodone did not affect locomotor activity or rearing. Sex differences were observed; the females traveled a greater distance and displayed more rearing than the males. In conclusion, oxycodone decreases anxiety-like behavior in rats, and oxycodone has a greater anxiolytic-like effect in males than females.

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Estimation of the Level of Anxiety in Rats: Differences in Results of Open-Field Test, Elevated Plus-Maze Test, and Vogel’s Conflict Test

Bulletin of Experimental Biology and Medicine ◽

10.1007/s10517-013-2136-y ◽

2013 ◽

Vol 155 (3) ◽

pp. 295-297 ◽

Cited By ~ 23

Author(s):

S. K. Sudakov ◽

G. A. Nazarova ◽

E. V. Alekseeva ◽

V. G. Bashkatova

Keyword(s):

Open Field ◽

Field Test ◽

Elevated Plus Maze ◽

Open Field Test ◽

Elevated Plus Maze Test ◽

Maze Test ◽

Plus Maze ◽

Conflict Test ◽

Vogel's Conflict Test

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A Novel Nutraceutical Formulation Can Improve Motor Activity and Decrease the Stress Level in a Murine Model of Middle-Age Animals

Journal of Clinical Medicine ◽

10.3390/jcm10040624 ◽

2021 ◽

Vol 10 (4) ◽

pp. 624

Author(s):

Dimitris Tsoukalas ◽

Ovidiu Zlatian ◽

Mihaela Mitroi ◽

Elisavet Renieri ◽

Aristidis Tsatsakis ◽

...

Keyword(s):

Open Field ◽

Field Test ◽

Elevated Plus Maze ◽

Open Field Test ◽

Centella Asiatica ◽

The Elderly ◽

Dependent Manner ◽

Elevated Plus Maze Test ◽

Maze Test ◽

Plus Maze

Ageing is a genetically programmed physiological process that is modulated by numerous environmental factors, associated with decreasing physiological function, decreasing reproductive rate and increasing age-related mortality rate. Maintaining mobility performance and physical function in the elderly is the main objective of the successful ageing concept. In this study, we aimed to evaluate the beneficial effect of a novel nutraceutical formulation containing Centella asiatica L. extract, vitamin C, zinc and vitamin D3 (as cholecalciferol) on motor activity and anxiety with the use of a murine model of old animals, as a means of providing proof for clinical use in the elderly, for enhancing physical strength and improving life quality. Eighteen Sprague Dawley 18 months old male rats were divided into three groups and received corn oil (the control group) or 1 capsule/kg bw Reverse supplement (treatment group 1) or 2 capsules/kg bw Reverse supplement (treatment group 2), for a period of 3 months. The Reverse supplement (Natural Doctor S.A, Athens, Greece) contains 9 mg Centella asiatica L. extract, vitamin C (200 mg as magnesium ascorbate), zinc (5 mg as zinc citrate), vitamin D3 (50 µg as cholecalciferol) per capsule. Before and after the treatment, the motor function and behavioral changes for anxiety and depression were evaluated using the open-field test, elevated plus-maze test and rotarod test. The supplementation with Reverse (Natural Doctor S.A) supplement can improve the locomotor activity in old rats in a dose-dependent manner, as demonstrated by an increase in the latency to leave from the middle square, in the number of rearings in the open field test, in the time spent in the open arms and time spent in the center in the elevated plus-maze test and the latency to all in all three consecutive trials in the rotarod test. Stress also decreased significantly in a dose-dependent manner, following the treatment with Reverse supplement, as was demonstrated by the decrease in the number of groomings at the open field test and time spent in the dark and the number of groomings at the elevated plus-maze test.

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GPR30 activation decreases anxiety in the open field test but not in the elevated plus maze test in female mice

Brain and Behavior ◽

10.1002/brb3.197 ◽

2013 ◽

Vol 4 (1) ◽

pp. 51-59 ◽

Cited By ~ 52

Author(s):

Divya Anchan ◽

Sara Clark ◽

Kevin Pollard ◽

Nandini Vasudevan

Keyword(s):

Open Field ◽

Field Test ◽

Elevated Plus Maze ◽

Open Field Test ◽

Female Mice ◽

Elevated Plus Maze Test ◽

Maze Test ◽

Plus Maze

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Sex differences in the elevated plus-maze test and large open field test in adult Wistar rats

Pharmacology Biochemistry and Behavior ◽

10.1016/j.pbb.2021.173168 ◽

2021 ◽

Vol 204 ◽

pp. 173168

Author(s):

Parker Knight ◽

Ranjithkumar Chellian ◽

Ryann Wilson ◽

Azin Behnood-Rod ◽

Stefany Panunzio ◽

...

Keyword(s):

Sex Differences ◽

Open Field ◽

Field Test ◽

Wistar Rats ◽

Elevated Plus Maze ◽

Open Field Test ◽

Elevated Plus Maze Test ◽

Maze Test ◽

Plus Maze

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Synergistic Action of Sodium Selenite with some Antidepressants and Diazepam in Mice

Pharmaceutics ◽

10.3390/pharmaceutics10040270 ◽

2018 ◽

Vol 10 (4) ◽

pp. 270

Author(s):

Ewa Kędzierska ◽

Lila Dąbkowska ◽

Paweł Obierzyński ◽

Magdalena Polakowska ◽

Ewa Poleszak ◽

...

Keyword(s):

Locomotor Activity ◽

Sodium Selenite ◽

Elevated Plus Maze ◽

Synergistic Action ◽

Elevated Plus Maze Test ◽

Maze Test ◽

Plus Maze ◽

Immobility Time ◽

Combined Administration ◽

Antidepressant Action

Background: The antidepressant and anxiolytic effects of selenium (Se) have been proven in many studies. This work was aimed at confirming these activities of its inorganic form—sodium selenite—and examining the possible synergy of action with antidepressants and diazepam. Methods: The antidepressant- and anxiolytic-like activity of Se was assessed using forced swim tests (FSTs) and elevated plus-maze test (EPMs). Spontaneous locomotor activity was measured using photoresistor actimeters. The experiments were conducted on male Albino Swiss mice. Results: Sodium selenite (0.5 mg/kg) reduced the immobility time in the FSTs and extended time spent in the open arms of EPMs without affecting locomotor activity The combined administration of Se at an ineffective dose (0.25 mg/kg) together with imipramine (15 mg/kg), fluoxetine (5 mg/kg), tianeptine (10 mg/kg), but not with reboxetine (2.5 mg/kg), resulted in a reduction of immobility time in FSTs, and with a threshold dose of diazepam (0.25 mg/kg) led to the prolongation of time spent in the open arms of the EPM. Moreover, the antidepressant-like effect of Se (0.5 mg/kg) was significantly reduced by pretreatment with p-chlorophenylalanine (100 mg/kg). Conclusions: The results may indicate the participation of serotonergic transmission to antidepressant action of Se and GABA-ergic transmission to its anxiolytic effects.

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The novel dipeptide ligand of TASP

Доклады Академии наук ◽

10.31857/s0869-56524842228-232 ◽

2019 ◽

Vol 484 (2) ◽

pp. 228-232

Author(s):

O. A. Deeva ◽

A. S. Pantileev ◽

I. V. Rybina ◽

M. A. Yarkova ◽

T. A. Gudasheva ◽

...

Keyword(s):

Elevated Plus Maze ◽

Open Field Test ◽

Anxiolytic Activity ◽

The Novel ◽

Minimum Effective Dose ◽

Elevated Plus Maze Test ◽

Maze Test ◽

Plus Maze ◽

Order Of Magnitude ◽

Preliminary Administration

Using the previously obtained first dipeptide ligand TSPO the N‑carbobenzoxy-L‑tryptophanyl-L‑isoleucine amide (GD‑23) as a basis, the new dipeptide was synthesized — the N‑phenylpropionyl–L‑tryptophanyl-L‑leucine amide (GD‑102). GD‑102 expressed anxiolytic activity in the open field test in BALB/c mice and in the elevated plus maze test in ICR mice. The minimum effective dose of GD‑102 was an order of magnitude lower than that of GD‑23. Preliminary administration of the TSPO selective antagonist, compound PK11195, completely blocked the anxiolytic activity of GD‑102, that indicated the participation of TSPO in the realization of the anxiolytic action GD‑102. The results were confirmed by molecular docking data.

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