scholarly journals A phenotypic rescue approach identifies lineage regionalization defects in a mouse model of DiGeorge syndrome

2021 ◽  
Author(s):  
Gabriella Lania ◽  
Monica Franzese ◽  
Adachi Noritaka ◽  
Marchesa Bilio ◽  
Annalaura Russo ◽  
...  
Keyword(s):  
Mouse Model ◽  
Vitamin B12 ◽  
Aortic Arch ◽  
Digeorge Syndrome ◽  
Molecular Level ◽  
Cellular Distribution ◽  
Cell Distribution ◽  
Pharyngeal Apparatus ◽  
Phenotypic Rescue

ABSTRACTTBX1 is a key regulator of pharyngeal apparatus (PhAp) development. Vitamin B12 treatment partially rescues aortic arch patterning defects of Tbx1+/- embryos. Here we show that it also improves cardiac outflow tract septation and branchiomeric muscle anomalies of Tbx1 hypomorphic mutants. At molecular level, the in vivo vB12 treatment let us to identify genes that were dysregulated by Tbx1 haploinsufficiency and rescued by treatment. We found that SLUG, encoded by the rescued gene Snai2, identified a population of mesodermal cells that was partially overlapping with but distinct from ISL1+ and TBX1+ populations. In addition, SLUG+ cells were mislocalized and had a greater tendency to aggregate in Tbx1+/- and Tbx1-/- embryos and vB12 treatment restore cellular distribution. Adjacent neural crest-derived mesenchymal cells, which do not express TBX1, were also affected, showing enhanced segregation from cardiopharyngeal mesodermal cells. We propose that TBX1 regulates cell distribution in core mesoderm and the arrangement of multiple lineages within the PhAp.

scholarly journals Vitamin B12 ameliorates the phenotype of a mouse model of DiGeorge syndrome

2017 ◽  
Vol 26 (22) ◽  
pp. 4540-4540
Author(s):  
Gabriella Lania ◽  
Alberto Bresciani ◽  
Monica Bisbocci ◽  
Alessandra Francone ◽  
Vincenza Colonna ◽  
...  
Keyword(s):  
Mouse Model ◽  
Vitamin B12 ◽  
Digeorge Syndrome

scholarly journals Vitamin B12 ameliorates the phenotype of a mouse model of DiGeorge syndrome

2016 ◽  
pp. ddw267
Author(s):  
Gabriella Lania ◽  
Alberto Bresciani ◽  
Monica Bisbocci ◽  
Alessandra Francone ◽  
Vincenza Colonna ◽  
...  
Keyword(s):  
Mouse Model ◽  
Vitamin B12 ◽  
Digeorge Syndrome

The influence of tumor-induced immune dysfunction on the immune cell distribution of gold nanoparticles in vivo

2017 ◽  
Vol 5 (8) ◽  
pp. 1531-1536 ◽  
Author(s):  
Chenxi Wu ◽  
Hongmei Chen ◽  
Xuan Wu ◽  
Xiuxiu Cong ◽  
Li Wang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Immune Cell ◽  
Immune Dysfunction ◽  
Cellular Distribution ◽  
Cell Distribution ◽  
Tumor Bearing

Tumor-induced immune dysfunction has been observed in tumor bearing mice. The nanoparticle cellular distribution is significantly affected by the immune dysfunction, compared with tumor free mice.

Bioavailability of Vitamin B12

2010 ◽  
Vol 80 (45) ◽  
pp. 330-335 ◽  
Author(s):  
Lindsay Helen Allen
Keyword(s):  
Vitamin B12 ◽  
Vitamin B12 Deficiency ◽  
The Elderly ◽  
Radioactive Isotopes ◽  
Animal Source Foods ◽  
Dietary Requirements ◽  
In Vivo Labeling ◽  
B12 Deficiency ◽  
Vitamin B12 Malabsorption

Vitamin B12 deficiency is common in people of all ages who consume a low intake of animal-source foods, including populations in developing countries. It is also prevalent among the elderly, even in wealthier countries, due to their malabsorption of B12 from food. Several methods have been applied to diagnose vitamin B12 malabsorption, including Schilling’s test, which is now used rarely, but these do not quantify percent bioavailability. Most of the information on B12 bioavailability from foods was collected 40 to 50 years ago, using radioactive isotopes of cobalt to label the corrinoid ring. The data are sparse, and the level of radioactivity required for in vivo labeling of animal tissues can be prohibitive. A newer method under development uses a low dose of radioactivity as 14C-labeled B12, with measurement of the isotope excreted in urine and feces by accelerator mass spectrometry. This test has revealed that the unabsorbed vitamin is degraded in the intestine. The percent bioavailability is inversely proportional to the dose consumed due to saturation of the active absorption process, even within the range of usual intake from foods. This has important implications for the assessment and interpretation of bioavailability values, setting dietary requirements, and interpreting relationships between intake and status of the vitamin.

In Vivo Monitoring of Human Platelets in a Humanised Rag2−/− γ-chain−/− Mouse Model

2015 ◽  
Vol 63 (S 01) ◽  
Author(s):  
C. Heim ◽  
S. Müller ◽  
B. Weigmann ◽  
M. Ramsperger-Gleixner ◽  
N. Koch ◽  
...  
Keyword(s):  
Mouse Model ◽  
Human Platelets ◽  
In Vivo Monitoring
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