scholarly journals The protective effect of Capheic acid phenyl ester on hepatic ischemia-reperfusion injury in cholestatic rats

2018 ◽  
Author(s):  
Arif Aslaner ◽  
Tugrul Cakir
Keyword(s):  
Protective Effect ◽  
Reperfusion Injury ◽  
Caffeic Acid ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Intraperitoneal Administration ◽  
Albino Rats ◽  
Myeloperoxidase Activity ◽  
Phenyl Ester ◽  
Hepatic Ischemia

PURPOSE: Caffeic acid phenyl ester (CAPE), which is an active component of propolis, has antioxidant, antiproliferative, immunomodulatory and anti-inflammatory properties and has been used for many years. The protective effect against ischemic reperfusion injury in the cholestatic liver is unknown and it is aimed to determine its effect in this study. MATERIALS AND METHODS: Three groups of 18 Wistar albino rats were divided into cholestasis, control and study groups with bile duct attachment. Intraperitoneal administration of caffeic acid phenyl ester at the dose of 30 mg/kg/ day started 15 days ago and continued until the second operation in the study group. On the seventh day, hepatic ischemia was performed for 30 minutes followed by reperfusion for 60 minutes. Serum, plasma and liver samples were taken. Laboratory analysis, tissue glutathione, malondialdehyde, and myeloperoxidase levels were evaluated. RESULTS: Significant reduction in the level of liver glutathione and a marked increase in malondialdehyde level and myeloperoxidase activity were observed in the cholestatic I / R group. After treatment, all parameters except serum bilirubin levels were reversed. CONCLUSIONS: Intraperitoneal administration of CAPE may improve liver function and may reduce inflammation and oxidative stress in cholestatic I / R injury.

scholarly journals The protective effect of diosmin on hepatic ischemia reperfusion injury: an experimental study

2013 ◽  
Vol 13 (4) ◽  
pp. 218 ◽  
Author(s):  
Yusuf Tanrikulu ◽  
Mefaret Şahin ◽  
Kemal Kismet ◽  
Sibel Serin Kilicoglu ◽  
Erdinc Devrim ◽  
...  
Keyword(s):  
Experimental Study ◽  
Protective Effect ◽  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Hepatic Ischemia ◽  
Hepatic Ischemia Reperfusion

Protective effect of heme oxygenase-1 on hepatic ischemia-reperfusion injury through inhibition of platelet adhesion to the sinusoids

2013 ◽  
Vol 28 (4) ◽  
pp. 700-706 ◽  
Author(s):  
Takafumi Tamura ◽  
Tadashi Kondo ◽  
Koichi Ogawa ◽  
Kiyoshi Fukunaga ◽  
Nobuhiro Ohkohchi
Keyword(s):  
Protective Effect ◽  
Reperfusion Injury ◽  
Heme Oxygenase ◽  
Platelet Adhesion ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Heme Oxygenase 1 ◽  
Hepatic Ischemia ◽  
Hepatic Ischemia Reperfusion

Protective effect of nimesulide against hepatic ischemia/reperfusion injury in rats: Effects on oxidant/antioxidants, DNA mutation and COX-1/COX-2 levels

2014 ◽  
Vol 66 (4) ◽  
pp. 647-652 ◽  
Author(s):  
Ismail Demiryilmaz ◽  
Mehmet Ibrahim Turan ◽  
Abdullah Kisaoglu ◽  
Mine Gulapoglu ◽  
Ismayil Yilmaz ◽  
...  
Keyword(s):  
Protective Effect ◽  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Hepatic Ischemia ◽  
Dna Mutation ◽  
Cox 2 ◽  
Hepatic Ischemia Reperfusion ◽  
Cox 1

scholarly journals The Effects of Two Anesthetics, Propofol and Sevoflurane, on Liver Ischemia/Reperfusion Injury

2016 ◽  
Vol 38 (4) ◽  
pp. 1631-1642 ◽  
Author(s):  
Zhijie Xu ◽  
Jingui Yu ◽  
Jianbo Wu ◽  
Feng Qi ◽  
Huanliang Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Treatment Group ◽  
Protective Effect ◽  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Liver Ischemia ◽  
Hepatic Ischemia ◽  
Group I ◽  
Hepatic Ischemia Reperfusion

Background: Propofol and sevoflurane are widely used in clinical anesthesia, and both have been reported to exert a protective effect in organ ischemia/reperfusion (IR). This study aims to investigate and compare the effects of propofol and sevoflurane on liver ischemia/reperfusion and the precise molecular mechanism. Methods and Materials: Rats were randomized into four groups: the sham group, I/R group, propofol treatment group (infused with 1% propofol at 500 µg· kg-1· min-1), and sevoflurane treatment group (infused with 3% (2 L/min) sevoflurane). The liver ischemia/reperfusion model was used to evaluate the hepatoprotective effect on ischemic injury. Liver enzyme leakage, liver cytokines and histopathological examination were used to evaluate the extent of hepatic ischemia/reperfusion injury. Oxidative stress was investigated by evaluating the levels of Malondialdehyde(MDA), Superoxide Dismutase(SOD) and NO. The terminal dexynucleotidyl transferase(TdT)-mediated dUTP nick end labeling (TUNEL) assay and western blot were applied to detect apoptosis in the ischemic liver tissue and its mechanism. Results: Both propofol and sevoflurane attenuated the extent of hepatic ischemia/reperfusion injury which is evident from the hisopathological studies and alterations in liver enzymes such as AST and LDH by inhibiting Nuclear factor kappa B (NFκB) activation and subsequent alterations in inflammatory cytokines interleukin-1(IL-1), interleukin-6(IL-6), tumor necrosis factor-alpha (TNF-a) and increased IL10 release. Propofol exhibited a similar protective effect and a lower IL-1 release, while sevoflurane decreased TNF-a leakage more significantly. Meanwhile, oxidative stress was attenuated by reduced MDA and NO and elevated SOD release. The expression of antiapoptotic protein Bcl-2 and Bcl-xl were enhanced while that of apoptotic protein Bax and Bak were reduced by both propofol and sevoflurane to regulate hepatic apoptosis. In addition, propofol downregulated the phosphorylation of AKT and Bad protein, while sevoflurane downregulated the phosphorylation of p38. In addition, Both the treatments had no effect on the expression of AKT, Bad and p38. Conclusion: Both propofol and sevoflurane can protect the liver from ischemia/reperfusion injury by modulating the inflammatory responses reducing oxidative stress and liver apoptosis.

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