Early changes in the urine proteome in a rat liver tumor model

AbstractUrine, as a potential biomarker source among the body fluids, can accumulate many changes in the body due to the lack of a mechanism to maintain a homeostatic state. Previous studies have demonstrated that proteomic technology can find many potential biomarkers to reflect different diseases in the urine. This study aims to detect early changes in the urinary proteome in a rat liver tumor model. The tumor model was established with the Walker-256 carcinosarcoma cell line (W256). Compared to before the injection, ninety-five differential proteins were significantly changed in the experimental rats. At day 3, twelve proteins were identified in the absence of pathological changes, and four of them were altered at all four time-points (B2MG, VCAM1, HA11, and LG3BP). Seven had previously been associated with liver cancer. At day 5, fifty-two differential proteins were identified. At day 7 and day 11, there was a significant decrease in the body weight of the rats, and tumor tissue was observed in the liver. Fifty-two and forty differential proteins were changed significantly at day 7 and day 11, respectively. Of the proteins that were identified at these three time-points, and twenty-four were reported to be associated with liver cancer. Comparing the differential urinary proteins and biological processes of liver tumor model with those in different models of W256 grown in other organs, specific differential protein patterns were found among the four models, which indicates that the differential urinary proteins can reflect the differences when the same tumor cell grown in different organs.SignificanceThis study demonstrated that (1) the rat liver tumor model caused early changes in urinary proteins may give new insight into the early diagnosis of liver cancer; (2) the same tumor cell grown in different organs can be reflected in differential urinary proteins.

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Early changes in the urine proteome in a rat liver tumour model

PeerJ ◽

10.7717/peerj.8462 ◽

2020 ◽

Vol 8 ◽

pp. e8462

Author(s):

Yameng Zhang ◽

Yufei Gao ◽

Youhe Gao

Keyword(s):

Rat Liver ◽

Protein Pattern ◽

Liver Tumour ◽

The Body ◽

Urinary Proteins ◽

Tumour Model ◽

Potential Biomarker ◽

Differential Proteins ◽

Walker 256 ◽

Homeostatic State

Background Urine, as a potential biomarker source among body fluids, can accumulate many early changes in the body due to the lack of mechanisms to maintain a homeostatic state. This study aims to detect early changes in the urinary proteome in a rat liver tumour model. Methods The tumour model was established with the Walker-256 carcinosarcoma cell line (W256). Urinary proteins at days 3, 5, 7 and 11 were profiled by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Compared with controls, differential proteins were selected. Associations of differential proteins with cancer were retrieved. Results At days 3, 5, 7 and 11, five, fifteen, eleven and twelve differential proteins were identified, respectively. Some of the differential proteins were reported to be associated with liver cancer. This differential urinary protein pattern was different from the patterns in W256 subcutaneous, lung metastasis and intracerebral tumour models. Conclusions This study demonstrates that (1) early changes in urinary proteins can be found in the rat liver tumour model; (2) urinary proteins can be used to differentiate the same tumour cells grown in different organs.

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Multimodality imaging to assess immediate response to irreversible electroporation in a rat liver tumor model

Journal of Vascular and Interventional Radiology ◽

10.1016/j.jvir.2013.01.294 ◽

2013 ◽

Vol 24 (4) ◽

pp. S119

Author(s):

Y. Zhang ◽

S.B. White ◽

J. Nicolai ◽

D.L. West ◽

Z. Zhang ◽

...

Keyword(s):

Rat Liver ◽

Liver Tumor ◽

Multimodality Imaging ◽

Irreversible Electroporation ◽

Tumor Model ◽

Immediate Response

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Biodistribution and Bioactivity of Tetra-pegylated Meta-tetra(hydroxyphenyl)chlorin Compared to Native Meta-tetra(hydroxyphenyl)chlorin in a Rat Liver Tumor Model

Photochemistry and Photobiology ◽

10.1562/0031-8655(2000)0710211babotp2.0.co2 ◽

2007 ◽

Vol 71 (2) ◽

pp. 211-217 ◽

Cited By ~ 1

Author(s):

Jeroen P. Rovers ◽

Anne E. Saarnak ◽

Martin Jode ◽

Hendricus J. C. M. Sterenborg ◽

Onno T. Terpstra ◽

...

Keyword(s):

Rat Liver ◽

Liver Tumor ◽

Tumor Model

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Dynamic urinary proteomic analysis in a Walker 256 intracerebral tumor model

10.1101/481697 ◽

2018 ◽

Cited By ~ 5

Author(s):

Linpei Zhang ◽

Yuqiu Li ◽

Wenshu Meng ◽

Yanying Ni ◽

Youhe Gao

Keyword(s):

Tumor Cells ◽

Early Stage ◽

Clinical Manifestations ◽

Tumor Model ◽

Protein Patterns ◽

Differential Proteins ◽

Walker 256 ◽

The Difference ◽

Intracerebral Tumor ◽

The Brain

AbstractPatients with primary and metastatic brain cancer have an extremely poor prognosis, mostly due to the late diagnosis of disease. Urine, which lacks homeostatic mechanisms, is an ideal biomarker source that accumulates early and highly sensitive changes to provides information about the early stage of disease. A rat model mimicking the local tumor growth process in the brain was established with intracerebral Walker 256 (W256) cell injection. Urine samples were collected on days 3, 5 and 8 after injection and then analyzed by LC-MS/MS. In the intracerebral W256 model, no obvious clinical manifestations changes or abnormal MRI signals were found on days 3 and 5; at these time points, nine proteins were changed significantly in the urine of all 8 tumor rats. On day 8, when tumors were detected by MRI, twenty-five differential proteins were identified, including 10 proteins that have been reported to be closely related to tumor metastasis or brain tumors. The differential urinary proteomes were compared with those from the subcutaneous W256 model and the intracerebral C6 model. Few differential proteins overlapped. Specific differential protein patterns were observed among the three models, indicating that the urinary proteome can reflect the difference when tumor cells with different growth characteristics are inoculated into the brain and when identical tumor cells are inoculated into different areas, specifically, the subcutis and the brain.

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