scholarly journals Biomolecular Contrast Agents for Optical Coherence Tomography

2019 ◽  
Author(s):  
George J. Lu ◽  
Li-dek Chou ◽  
Dina Malounda ◽  
Amit K. Patel ◽  
Derek S. Welsbie ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Contrast Agents ◽  
Fluorescent Protein ◽  
Mouse Retina ◽  
Optical Coherence ◽  
Gas Vesicles ◽  
Cellular Functions ◽  
Green Fluorescent

ABSTRACTOptical coherence tomography (OCT) has gained wide adoption in biological and medical imaging due to its exceptional tissue penetration, 3D imaging speed and rich contrast. However, OCT plays a relatively small role in molecular and cellular imaging due to the lack of suitable biomolecular contrast agents. In particular, while the green fluorescent protein has provided revolutionary capabilities to fluorescence microscopy by connecting it to cellular functions such as gene expression, no equivalent reporter gene is currently available for OCT. Here we introduce gas vesicles, a unique class of naturally evolved gas-filled protein nanostructures, as the first genetically encodable OCT contrast agents. The differential refractive index of their gas compartments relative to surrounding aqueous tissue and their nanoscale motion enables gas vesicles to be detected by static and dynamic OCT at picomolar concentrations. Furthermore, the OCT contrast of gas vesicles can be selectively erasedin situwith ultrasound, allowing unambiguous assignment of their location. In addition, gas vesicle clustering modulates their temporal signal, enabling the design of dynamic biosensors. We demonstrate the use of gas vesicles as reporter genes in bacterial colonies and as purified contrast agentsin vivoin the mouse retina. Our results expand the utility of OCT as a unique photonic modality to image a wider variety of cellular and molecular processes.

scholarly journals In vivo imaging of coral tissue and skeleton with optical coherence tomography

2017 ◽  
Vol 14 (128) ◽  
pp. 20161003 ◽  
Author(s):  
Daniel Wangpraseurt ◽  
Camilla Wentzel ◽  
Steven L. Jacques ◽  
Michael Wagner ◽  
Michael Kühl
Keyword(s):  
Optical Coherence Tomography ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Coral Tissue ◽  
Coral Host ◽  
Live Coral ◽  
Optical Coherence ◽  
Linear Contraction ◽  
Green Fluorescent

Application of optical coherence tomography (OCT) for in vivo imaging of tissue and skeleton structure of intact living corals enabled the non-invasive visualization of coral tissue layers (endoderm versus ectoderm), skeletal cavities and special structures such as mesenterial filaments and mucus release from intact living corals. Coral host chromatophores containing green fluorescent protein-like pigment granules appeared hyper-reflective to near-infrared radiation allowing for excellent optical contrast in OCT and a rapid characterization of chromatophore size, distribution and abundance. In vivo tissue plasticity could be quantified by the linear contraction velocity of coral tissues upon illumination resulting in dynamic changes in the live coral tissue surface area, which varied by a factor of 2 between the contracted and expanded state of a coral. Our study provides a novel view on the in vivo organization of coral tissue and skeleton and highlights the importance of microstructural dynamics for coral ecophysiology.

Gold Nanobipyramids as Second Near Infrared Optical Coherence Tomography Contrast Agents for in Vivo Multiplexing Studies

Nano Letters ◽  
2019 ◽  
Vol 20 (1) ◽  
pp. 101-108 ◽  
Author(s):  
Peng Si ◽  
Saba Shevidi ◽  
Edwin Yuan ◽  
Ke Yuan ◽  
Ziv Lautman ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Contrast Agents ◽  
Near Infrared ◽  
Optical Coherence ◽  
Gold Nanobipyramids

In vivo in situ en face optical coherence tomography imaging of chick embryos

2009 ◽  
Author(s):  
Michael Leitner ◽  
Joana Castanheira ◽  
Luís Ferreira ◽  
Mónica Ferreira ◽  
Isabel Palmeirim ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Chick Embryos ◽  
Optical Coherence ◽  
Tomography Imaging ◽  
En Face ◽  
Optical Coherence Tomography Imaging

In vivo imaging of gold nanorod contrast agents using photothermal optical coherence tomography

2013 ◽  
Author(s):  
Jason M. Tucker-Schwartz ◽  
Travis A. Meyer ◽  
Chetan A. Patil ◽  
Craig L. Duvall ◽  
Melissa C. Skala
Keyword(s):  
Optical Coherence Tomography ◽  
Contrast Agents ◽  
In Vivo Imaging ◽  
Gold Nanorod ◽  
Optical Coherence

scholarly journals In vivo photothermal optical coherence tomography of endogenous and exogenous contrast agents in the eye

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Maryse Lapierre-Landry ◽  
Andrew Y. Gordon ◽  
John S. Penn ◽  
Melissa C. Skala
Keyword(s):  
Optical Coherence Tomography ◽  
Contrast Agents ◽  
Optical Coherence

scholarly journals A Spectral Demixing Model for Triplex In Vivo Imaging of Optical Coherence Tomography Contrast Agents

ACS Photonics ◽  
2020 ◽  
Vol 7 (4) ◽  
pp. 893-900
Author(s):  
Edwin Yuan ◽  
Peng Si ◽  
Yonatan Winetraub ◽  
Saba Shevidi ◽  
Adam de la Zerda
Keyword(s):  
Optical Coherence Tomography ◽  
Contrast Agents ◽  
In Vivo Imaging ◽  
Optical Coherence

scholarly journals In Vivo Study of Trichoderma-Pathogen-Plant Interactions, Using Constitutive and Inducible Green Fluorescent Protein Reporter Systems

2004 ◽  
Vol 70 (5) ◽  
pp. 3073-3081 ◽  
Author(s):  
Zexun Lu ◽  
Riccardo Tombolini ◽  
Sheridan Woo ◽  
Susanne Zeilinger ◽  
Matteo Lorito ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Plant Pathogens ◽  
Fluorescent Protein ◽  
Morphological Changes ◽  
Critical Role ◽  
Pythium Ultimum ◽  
Confocal Scanning Laser Microscopy ◽  
Green Fluorescent

ABSTRACT Plant tissue colonization by Trichoderma atroviride plays a critical role in the reduction of diseases caused by phytopathogenic fungi, but this process has not been thoroughly studied in situ. We monitored in situ interactions between gfp-tagged biocontrol strains of T. atroviride and soilborne plant pathogens that were grown in cocultures and on cucumber seeds by confocal scanning laser microscopy and fluorescence stereomicroscopy. Spores of T. atroviride adhered to Pythium ultimum mycelia in coculture experiments. In mycoparasitic interactions of T. atroviride with P. ultimum or Rhizoctonia solani, the mycoparasitic hyphae grew alongside the pathogen mycelia, and this was followed by coiling and formation of specialized structures similar to hooks, appressoria, and papillae. The morphological changes observed depended on the pathogen tested. Branching of T. atroviride mycelium appeared to be an active response to the presence of the pathogenic host. Mycoparasitism of P. ultimum by T. atroviride occurred on cucumber seed surfaces while the seeds were germinating. The interaction of these fungi on the cucumber seeds was similar to the interaction observed in coculture experiments. Green fluorescent protein expression under the control of host-inducible promoters was also studied. The induction of specific Trichoderma genes was monitored visually in cocultures, on plant surfaces, and in soil in the presence of colloidal chitin or Rhizoctonia by confocal microscopy and fluorescence stereomicroscopy. These tools allowed initiation of the mycoparasitic gene expression cascade to be monitored in vivo.

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