Comparative evaluation of Aspergillus niger strains for endogenous pectin depolymerization capacity and suitability for D-galacturonic acid production

AbstractPectinaceous agricultural residues rich in d-galacturonic acid (d-GalA), such as sugar beet pulp, are considered as promising feedstocks for waste-to-value conversions. Aspergillus niger is known for its strong pectinolytic activity. However, while specialized strains for production of citric acid or proteins are openly available, this is not the case for the production of pectinases. We therefore systematically compared the pectinolytic capabilities of six A. niger strains (ATCC 1015, ATCC 11414, NRRL 3122, CBS 513.88, NRRL 3, N402) using controlled batch cultivations in stirred-tank bioreactors. A. niger ATCC 11414 showed the highest polygalacturonase activity, specific protein secretion and a suitable morphology. Furthermore, d-GalA release from sugar beet pulp was 75% higher compared to the standard lab strain A. niger N402. Our study therefore presents a robust initial strain selection to guide future process improvement of d-GalA production from agricultural residues and identifies the most suitable base strain for further genetic optimizations.

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Kinetics of galacturonic acid release from sugar-beet pulp

Enzyme and Microbial Technology ◽

10.1016/j.enzmictec.2003.12.008 ◽

2004 ◽

Vol 34 (5) ◽

pp. 505-512 ◽

Cited By ~ 18

Author(s):

I.-E. Baciu ◽

H.-J. Jördening

Keyword(s):

Sugar Beet ◽

Galacturonic Acid ◽

Sugar Beet Pulp ◽

Beet Pulp ◽

Acid Release ◽

Kinetics Of

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Polygalacturonase Production by Aspergillus Niger Solid State Fermentation on Barley Bran and Sugar Beet Pulp Mixture

Advanced Journal of Chemistry-Section A ◽

10.33945/sami/ajca.2020.3.13 ◽

2020 ◽

Vol 3 (3) ◽

pp. 350-357

Keyword(s):

Solid State ◽

Aspergillus Niger ◽

Sugar Beet ◽

Solid State Fermentation ◽

Sugar Beet Pulp ◽

Beet Pulp ◽

Barley Bran ◽

Polygalacturonase Production

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Pectinolytic activity ofClostridium thermocellum: Its use for anaerobic fermentation of sugar beet pulp

Applied Microbiology and Biotechnology ◽

10.1007/bf02346055 ◽

1986 ◽

Vol 23 (6) ◽

pp. 434-437 ◽

Cited By ~ 15

Author(s):

H. E. Spinnler ◽

B. Lavigne ◽

H. Blachere

Keyword(s):

Sugar Beet ◽

Anaerobic Fermentation ◽

Sugar Beet Pulp ◽

Pectinolytic Activity ◽

Beet Pulp

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Enzymatic saccharification of sugar beet pulp for the production of galacturonic acid and arabinose; a study on the impact of the formation of recalcitrant oligosaccharides

Bioresource Technology ◽

10.1016/j.biortech.2012.10.126 ◽

2013 ◽

Vol 128 ◽

pp. 518-525 ◽

Cited By ~ 42

Author(s):

A.G.M. Leijdekkers ◽

J.P.M. Bink ◽

S. Geutjes ◽

H.A. Schols ◽

H. Gruppen

Keyword(s):

Sugar Beet ◽

Galacturonic Acid ◽

Enzymatic Saccharification ◽

Sugar Beet Pulp ◽

Beet Pulp ◽

The Impact

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Complete Utilization of the Major Carbon Sources Present in Sugar Beet Pulp Hydrolysates by the Oleaginous Red Yeasts Rhodotorula toruloides and R. mucilaginosa

Journal of Fungi ◽

10.3390/jof7030215 ◽

2021 ◽

Vol 7 (3) ◽

pp. 215

Author(s):

Luís Martins ◽

Margarida Palma ◽

Angel Angelov ◽

Elke Nevoigt ◽

Wolfgang Liebl ◽

...

Keyword(s):

Acetic Acid ◽

Sugar Beet ◽

Galacturonic Acid ◽

Fruits And Vegetables ◽

Carbon Sources ◽

Value Added ◽

Sugar Beet Pulp ◽

Industrial Residues ◽

Industrial Processing ◽

Beet Pulp

Agro-industrial residues are low-cost carbon sources (C-sources) for microbial growth and production of value-added bioproducts. Among the agro-industrial residues available, those rich in pectin are generated in high amounts worldwide from the sugar industry or the industrial processing of fruits and vegetables. Sugar beet pulp (SBP) hydrolysates contain predominantly the neutral sugars d-glucose, l-arabinose and d-galactose, and the acidic sugar d-galacturonic acid. Acetic acid is also present at significant concentrations since the d-galacturonic acid residues are acetylated. In this study, we have examined and optimized the performance of a Rhodotorula mucilaginosa strain, isolated from SBP and identified at the molecular level during this work. This study was extended to another oleaginous red yeast species, R. toruloides, envisaging the full utilization of the C-sources from SBP hydrolysate (at pH 5.0). The dual role of acetic acid as a carbon and energy source and as a growth and metabolism inhibitor was examined. Acetic acid prevented the catabolism of d-galacturonic acid and l-arabinose after the complete use of the other C-sources. However, d-glucose and acetic acid were simultaneously and efficiently metabolized, followed by d-galactose. SBP hydrolysate supplementation with amino acids was crucial to allow d-galacturonic acid and l-arabinose catabolism. SBP valorization through the production of lipids and carotenoids by Rhodotorula strains, supported by complete catabolism of the major C-sources present, looks promising for industrial implementation.

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