scholarly journals Complete Utilization of the Major Carbon Sources Present in Sugar Beet Pulp Hydrolysates by the Oleaginous Red Yeasts Rhodotorula toruloides and R. mucilaginosa

2021 ◽  
Vol 7 (3) ◽  
pp. 215
Author(s):  
Luís Martins ◽  
Margarida Palma ◽  
Angel Angelov ◽  
Elke Nevoigt ◽  
Wolfgang Liebl ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Sugar Beet ◽  
Galacturonic Acid ◽  
Fruits And Vegetables ◽  
Carbon Sources ◽  
Value Added ◽  
Sugar Beet Pulp ◽  
Industrial Residues ◽  
Industrial Processing ◽  
Beet Pulp

Agro-industrial residues are low-cost carbon sources (C-sources) for microbial growth and production of value-added bioproducts. Among the agro-industrial residues available, those rich in pectin are generated in high amounts worldwide from the sugar industry or the industrial processing of fruits and vegetables. Sugar beet pulp (SBP) hydrolysates contain predominantly the neutral sugars d-glucose, l-arabinose and d-galactose, and the acidic sugar d-galacturonic acid. Acetic acid is also present at significant concentrations since the d-galacturonic acid residues are acetylated. In this study, we have examined and optimized the performance of a Rhodotorula mucilaginosa strain, isolated from SBP and identified at the molecular level during this work. This study was extended to another oleaginous red yeast species, R. toruloides, envisaging the full utilization of the C-sources from SBP hydrolysate (at pH 5.0). The dual role of acetic acid as a carbon and energy source and as a growth and metabolism inhibitor was examined. Acetic acid prevented the catabolism of d-galacturonic acid and l-arabinose after the complete use of the other C-sources. However, d-glucose and acetic acid were simultaneously and efficiently metabolized, followed by d-galactose. SBP hydrolysate supplementation with amino acids was crucial to allow d-galacturonic acid and l-arabinose catabolism. SBP valorization through the production of lipids and carotenoids by Rhodotorula strains, supported by complete catabolism of the major C-sources present, looks promising for industrial implementation.

Restructuring the Conventional Sugar Beet Industry into a Novel Biorefinery: Fractionation and Bioconversion of Sugar Beet Pulp into Succinic Acid and Value-Added Coproducts

2019 ◽  
Vol 7 (7) ◽  
pp. 6569-6579 ◽  
Author(s):  
Maria Alexandri ◽  
Roland Schneider ◽  
Harris Papapostolou ◽  
Dimitrios Ladakis ◽  
Apostolis Koutinas ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Succinic Acid ◽  
Value Added ◽  
Sugar Beet Pulp ◽  
Beet Pulp

scholarly journals An integrated biorefinery concept for conversion of sugar beet pulp into value-added chemicals and pharmaceutical intermediates

2017 ◽  
Vol 202 ◽  
pp. 415-431 ◽  
Author(s):  
Max Cárdenas-Fernández ◽  
Maria Bawn ◽  
Charlotte Hamley-Bennett ◽  
Penumathsa K. V. Bharat ◽  
Fabiana Subrizi ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Animal Feed ◽  
Value Added ◽  
Sugar Beet Pulp ◽  
High Yield ◽  
Integrated Biorefinery ◽  
Physico Chemical ◽  
Beet Pulp ◽  
Commercial Yeast ◽  
The Uk

Over 8 million tonnes of sugar beet are grown annually in the UK. Sugar beet pulp (SBP) is the main by-product of sugar beet processing which is currently dried and sold as a low value animal feed. SBP is a rich source of carbohydrates, mainly in the form of cellulose and pectin, including d-glucose (Glu), l-arabinose (Ara) and d-galacturonic acid (GalAc). This work describes the technical feasibility of an integrated biorefinery concept for the fractionation of SBP and conversion of these monosaccharides into value-added products. SBP fractionation is initially carried out by steam explosion under mild conditions to yield soluble pectin and insoluble cellulose fractions. The cellulose is readily hydrolysed by cellulases to release Glu that can then be fermented by a commercial yeast strain to produce bioethanol at a high yield. The pectin fraction can be either fully hydrolysed, using physico-chemical methods, or selectively hydrolysed, using cloned arabinases and galacturonases, to yield Ara-rich and GalAc-rich streams. These monomers can be separated using either Centrifugal Partition Chromatography (CPC) or ultrafiltration into streams suitable for subsequent enzymatic upgrading. Building on our previous experience with transketolase (TK) and transaminase (TAm) enzymes, the conversion of Ara and GalAc into higher value products was explored. In particular the conversion of Ara into l-gluco-heptulose (GluHep), that has potential therapeutic applications in hypoglycaemia and cancer, using a mutant TK is described. Preliminary studies with TAm also suggest GluHep can be selectively aminated to the corresponding chiral aminopolyol. The current work is addressing the upgrading of the remaining SBP monomer, GalAc, and the modelling of the biorefinery concept to enable economic and Life Cycle Analysis (LCA).

scholarly journals Physicochemical, Thermal and Rheological Properties of Pectin Extracted from Sugar Beet Pulp Using Subcritical Water Extraction Process

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1413
Author(s):  
Seyed Hadi Peighambardoust ◽  
Maryam Jafarzadeh-Moghaddam ◽  
Mirian Pateiro ◽  
José M. Lorenzo ◽  
Rubén Domínguez
Keyword(s):  
Sugar Beet ◽  
Corn Starch ◽  
Subcritical Water ◽  
Maximum Yield ◽  
Value Added ◽  
Extraction Process ◽  
Sugar Beet Pulp ◽  
Operational Parameters ◽  
Subcritical Water Extraction ◽  
Beet Pulp

The objective of this study was to characterize the properties of pectin extracted from sugar beet pulp using subcritical water (SWE) as compared to conventional extraction (CE). The research involved advanced modeling using response surface methodology and optimization of operational parameters. The optimal conditions for maximum yield of pectin for SWE and CE methods were determined by the central composite design. The optimum conditions of CE were the temperature of 90 °C, time of 240 min, pH of 1, and pectin recovery yield of 20.8%. The optimal SWE conditions were liquid-to-solid (L/S) ratio of 30% (v/w) at temperature of 130 °C for 20 min, which resulted in a comparable yield of 20.7%. The effect of obtained pectins on viscoamylograph pasting and DSC thermal parameters of corn starch was evaluated. The contents of galacturonic acid, degree of methylation, acetylation, and ferulic acid content were higher in the pectin extracted by SWE, while the molecular weight was lower. Similar chemical groups were characterized by FTIR in both SWE and CE pectins. Color attributes of both pectins were similar. Solutions of pectins at lower concentrations displayed nearly Newtonian behavior. The addition of both pectins to corn starch decreased pasting and DSC gelatinization parameters, but increased ΔH. The results offered a promising scalable approach to convert the beet waste to pectin as a value-added product using SWE with improved pectin properties.

scholarly journals Biomass Valorization: Sustainable Methods for the Production of Hemicellulolytic Catalysts from Thermoanaerobacterium thermostercoris strain BUFF

Resources ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 115
Author(s):  
Ilaria Finore ◽  
Ida Romano ◽  
Luigi Leone ◽  
Paola Di Donato ◽  
Barbara Nicolaus ◽  
...  
Keyword(s):  
Fruits And Vegetables ◽  
Carbon Sources ◽  
Value Added ◽  
Giant Reed ◽  
Foeniculum Vulgare ◽  
Anaerobic Microorganism ◽  
Biomass Valorization ◽  
Industrial Processing ◽  
Buffalo Dung ◽  
Selection Of

Processing and selection of fruits and vegetables generate high quantities of wastes that represent an economic and environmental issue for the agroindustry sector. According to the so-called “biorefinery” approach, this biomass can be exploited for the recovery of value-added molecules. In this study, the residues of industrial processing of tomato (Lycopersicon esculentum variety “Hybrid Rome”), fennel (Foeniculum vulgare), potato (Solanum tuberosum) and carrot (Daucus carota) were used as sole carbon sources to support cheap and sustainable microbial growth as well as the production of secondary metabolites (hydrogen and ethanol) by Thermoanaerobacterium thermostercoris strain BUFF, a thermophilic anaerobic microorganism isolated from buffalo-dung compost. Moreover, the use of hemicellulolytic enzymes of T. thermostercoris was assayed in the bioconversion reaction of the polymer fraction extracted from the rhizome of giant reed (Arundo donax) and of the leaves and stems of cardoon (Cynara cardunculus), dedicated non-food crops employed in energy supply.

scholarly journals Comparative evaluation of Aspergillus niger strains for endogenous pectin depolymerization capacity and suitability for D-galacturonic acid production

2019 ◽  
Author(s):  
Dominik Schäfer ◽  
Kevin Schmitz ◽  
Dirk Weuster-Botz ◽  
J. Philipp Benz
Keyword(s):  
Aspergillus Niger ◽  
Sugar Beet ◽  
Galacturonic Acid ◽  
Specific Protein ◽  
Agricultural Residues ◽  
Sugar Beet Pulp ◽  
Pectinolytic Activity ◽  
Beet Pulp ◽  
Base Strain ◽  
Polygalacturonase Activity

AbstractPectinaceous agricultural residues rich in d-galacturonic acid (d-GalA), such as sugar beet pulp, are considered as promising feedstocks for waste-to-value conversions. Aspergillus niger is known for its strong pectinolytic activity. However, while specialized strains for production of citric acid or proteins are openly available, this is not the case for the production of pectinases. We therefore systematically compared the pectinolytic capabilities of six A. niger strains (ATCC 1015, ATCC 11414, NRRL 3122, CBS 513.88, NRRL 3, N402) using controlled batch cultivations in stirred-tank bioreactors. A. niger ATCC 11414 showed the highest polygalacturonase activity, specific protein secretion and a suitable morphology. Furthermore, d-GalA release from sugar beet pulp was 75% higher compared to the standard lab strain A. niger N402. Our study therefore presents a robust initial strain selection to guide future process improvement of d-GalA production from agricultural residues and identifies the most suitable base strain for further genetic optimizations.

Valorization of Sugar Beet Pulp to Value-Added Products: A Review

2021 ◽  
pp. 126580
Author(s):  
Zeba Usmani ◽  
Minaxi Sharma ◽  
Deepti Diwan ◽  
Manikant Tripathi ◽  
Eric Whale ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Value Added ◽  
Sugar Beet Pulp ◽  
Beet Pulp ◽  
Value Added Products

Obtaining of sorbing material based on sugar beet pulp

2019 ◽  
pp. 10-16
Author(s):  
S.V. Meshcheryakov ◽  
◽  
I.S. Eremin ◽  
D.O. Sidorenko ◽  
M.S. Kotelev ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Sugar Beet Pulp ◽  
Beet Pulp

Kinetic and thermodynamic study of the adsorption of calcium onto sugar beet pulp

2016 ◽  
pp. 565-570
Author(s):  
Huang Qin ◽  
Zhu Si-ming ◽  
Zeng Di ◽  
Yu Shu-juan
Keyword(s):  
Sugar Beet ◽  
Adsorption Equilibrium ◽  
Ph Value ◽  
Hard Water ◽  
Sugar Beet Pulp ◽  
Order Kinetic ◽  
Factors Affecting ◽  
Isotherm Equation ◽  
Equilibrium Data ◽  
Beet Pulp

Sugar beet pulp (SBP) was used as low value adsorbent for the removal of calcium from hard water. Batch experiments were conducted to determine the factors affecting adsorption of the process such as pH value and Ca concentration. The adsorption equilibrium of Ca2+ by the SBP is reached after 100min and a pseudo second-order kinetic model can describe the adsorption process. The initial concentrations of Ca varied from 927 to 1127mgCa2+/L. A dose of 30g/L sugar beet pulp was sufficient for the optimum removal of calcium. The overall uptake of Ca ions by sugar beet pulp has its maximum at pH=8. The adsorption equilibrium data fitted well with the Langmuir adsorption isotherm equation.

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