Imaginal Disc Regeneration: Something Old, Something New

2021 ◽  
pp. a040733
Author(s):  
Melanie I. Worley ◽  
Iswar K. Hariharan
Keyword(s):  
Imaginal Disc ◽  
Disc Regeneration

Gene expression during imaginal disc regeneration detected using enhancer-sensitive P-elements

Development ◽  
1993 ◽  
Vol 117 (4) ◽  
pp. 1287-1297 ◽  
Author(s):  
W.J. Brook ◽  
L.M. Ostafichuk ◽  
J. Piorecky ◽  
M.D. Wilkinson ◽  
D.J. Hodgetts ◽  
...  
Keyword(s):  
Cell Death ◽  
Imaginal Disc ◽  
Expression Patterns ◽  
Imaginal Discs ◽  
P Element ◽  
Temperature Sensitive ◽  
Lethal Mutant ◽  
Disc Regeneration ◽  
Lac Z ◽  
Regeneration Blastema

When imaginal disc fragments from Drosophila are cultured in adult female hosts, they either duplicate the part of the pattern specified by the fate map, or regenerate to replace the missing part. The new tissue is added by proliferation of a small number of cells from the cut edge, brought together when the wound heals to form a regeneration blastema. Specification of the new pattern has been explained by assuming interactions among cells of different positional value in the regeneration blastema. In order to identify genes which might mediate these events, we screened over eight hundred independently isolated autosomal insertions of an enhancer-sensitive P-element, for altered lac-z expression in regenerating discs following cell death induced by a temperature-sensitive cell-lethal mutation. Two further screens divided the positive lines into four groups based on appropriate timing of the lac-z response in the cell-lethal mutant background and the expected response to an alternate source of cell death. Expression in wing disc fragments cultured in vivo was most frequent in the target class defined by the screens. In this direct test, lac-z expression was found in 23 lines and in most cases was spatially and temporally correlated with the formation of the regeneration blastema. Our results suggest a very substantial transcriptional response during the early stages of imaginal disc regeneration. lac-z expression in control imaginal discs, embryos and adult ovaries of the positive lines was also assayed. The selected insertions included: a small class expressed only in discs undergoing regeneration and apparently not at any other stage, possibly representing genes active exclusively in regeneration; a larger class expressed in the embryo or during oogenesis, but not normally in imaginal discs, as expected for functions recruited from earlier stages of the developmental program; and finally a class with spatially patterned expression in normal discs. This class included several insertions with expression associated with compartment boundaries, including one at the decapentaplegic (dpp), and one at the crumbs (crb) locus, a growth factor homologue, and an EGF-repeat gene respectively. Some of the expression patterns observed in cultured disc fragments provide evidence for cell communication in the regeneration blastema.

scholarly journals Growth Coordination During Drosophila melanogaster Imaginal Disc Regeneration Is Mediated by Signaling Through the Relaxin Receptor Lgr3 in the Prothoracic Gland

Genetics ◽  
2016 ◽  
Vol 204 (2) ◽  
pp. 703-709 ◽  
Author(s):  
J. S. Jaszczak ◽  
J. B. Wolpe ◽  
R. Bhandari ◽  
R. G. Jaszczak ◽  
A. Halme
Keyword(s):  
Drosophila Melanogaster ◽  
Imaginal Disc ◽  
Prothoracic Gland ◽  
Disc Regeneration ◽  
Relaxin Receptor

scholarly journals The relaxin receptor Lgr3 mediates growth coordination and developmental delay during Drosophila melanogaster imaginal disc regeneration

2015 ◽  
Author(s):  
Jacob S. Jaszczak ◽  
Jacob B. Wolpe ◽  
Rajan Bhandari ◽  
Rebecca G. Jaszczak ◽  
Adrian Halme
Keyword(s):  
Drosophila Melanogaster ◽  
Developmental Delay ◽  
Imaginal Disc ◽  
Imaginal Discs ◽  
Prothoracic Gland ◽  
Disc Regeneration ◽  
Relaxin Family ◽  
Dependent Growth ◽  
Oxide Synthase ◽  
Relaxin Receptor

Damage to Drosophila melanogaster imaginal discs activates a regeneration checkpoint that 1) extends larval development and 2) coordinates the regeneration of the damaged disc with the growth of undamaged discs. These two systemic responses to damage are both mediated by Dilp8, a member of the insulin/IGF/relaxin family of peptide hormones, which is released by regenerating imaginal discs. Growth coordination between regenerating and undamaged imaginal discs is dependent on Dilp8 activation of NOS in the prothoracic gland (PG), which slows the growth of undamaged discs by limiting ecdysone synthesis. Here we demonstrate that the Drosophila relaxin receptor homologue Lgr3, a leucine-rich repeat-containing G-protein coupled receptor, is required for Dilp8-dependent growth coordination and developmental delay during the regeneration checkpoint. Lgr3 regulates these responses to damage via distinct mechanisms in different tissues. Using tissue-specific RNAi disruption of Lgr3 expression, we show that Lgr3 functions in the PG upstream of nitric oxide synthase (NOS), and is necessary for NOS activation and growth coordination during the regeneration checkpoint. When Lgr3 is depleted from neurons, imaginal disc damage no longer produces either developmental delay or growth inhibition. To reconcile these discrete tissue requirements for Lgr3 during regenerative growth coordination, we demonstrate that Lgr3 activity in the both the CNS and PG is necessary for NOS activation in the PG following damage. Together, these results identify new roles for a relaxin receptor in mediating damage signaling to regulate growth and developmental timing.

scholarly journals Imaginal disc regeneration takes flight

2017 ◽  
Vol 48 ◽  
pp. 10-16 ◽  
Author(s):  
Iswar K Hariharan ◽  
Florenci Serras
Keyword(s):  
Imaginal Disc ◽  
Disc Regeneration

scholarly journals HORMONAL CONTROL OF IMAGINAL DISC REGENERATION INGALLERIA MELLONELLA(LEPIDOPTERA)

1969 ◽  
Vol 137 (2) ◽  
pp. 321-331 ◽  
Author(s):  
KORNATH MADHAVAN ◽  
HOWARD A. SCHNEIDERMAN
Keyword(s):  
Imaginal Disc ◽  
Hormonal Control ◽  
Disc Regeneration
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