scholarly journals Characterization of Three Maize Bacterial Artificial Chromosome Libraries toward Anchoring of the Physical Map to the Genetic Map Using High-Density Bacterial Artificial Chromosome Filter Hybridization

2002 ◽  
Vol 130 (4) ◽  
pp. 1686-1696 ◽  
Author(s):  
Young-Sun Yim ◽  
Georgia L. Davis ◽  
Ngozi A. Duru ◽  
Theresa A. Musket ◽  
Eric W. Linton ◽  
...  
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Genetic Map ◽  
Physical Map ◽  
Artificial Chromosome ◽  
High Density ◽  
Filter Hybridization

scholarly journals A high-density physical map of Sinorhizobium meliloti 1021 chromosome derived from bacterial artificial chromosome library

1999 ◽  
Vol 96 (16) ◽  
pp. 9357-9362 ◽  
Author(s):  
D. Capela ◽  
F. Barloy-Hubler ◽  
M.-T. Gatius ◽  
J. Gouzy ◽  
F. Galibert
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Bacterial Artificial Chromosome Library ◽  
Sinorhizobium Meliloti ◽  
Physical Map ◽  
Artificial Chromosome ◽  
High Density

Bacterial artificial chromosome–based physical map of Gibberella zeae (Fusarium graminearum)

Genome ◽  
2007 ◽  
Vol 50 (10) ◽  
pp. 954-962 ◽  
Author(s):  
Yueh-Long Chang ◽  
Seungho Cho ◽  
H. Corby Kistler ◽  
Chun-Sheng Hsieh ◽  
Gary J. Muehlbauer
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Fusarium Graminearum ◽  
Genetic Map ◽  
Genome Sequence ◽  
Restriction Enzyme ◽  
Physical Map ◽  
Artificial Chromosome ◽  
Gibberella Zeae ◽  
Restriction Enzyme Site ◽  
Insert Size

Fusarium graminearum is the primary causal pathogen of Fusarium head blight of wheat and barley. To accelerate genomic analysis of F. graminearum, we developed a bacterial artificial chromosome (BAC)–based physical map and integrated it with the genome sequence and genetic map. One BAC library, developed in the HindIII restriction enzyme site, consists of 4608 clones with an insert size of approximately 107 kb and covers about 13.5 genome equivalents. The other library, developed in the BamHI restriction enzyme site, consists of 3072 clones with an insert size of approximately 95 kb and covers about 8.0 genome equivalents. We fingerprinted 2688 clones from the HindIII library and 1536 clones from the BamHI library and developed a physical map of F. graminearum consisting of 26 contigs covering 39.2 Mb. Comparison of our map with the F. graminearum genome sequence showed that the size of our physical map is equivalent to the 36.1 Mb of the genome sequence. We used 31 sequence-based genetic markers, randomly spaced throughout the genome, to integrate the physical map with the genetic map. We also end-sequenced 17 BamHI BAC clones and identified 4 clones that spanned gaps in the genome sequence. Our new integrated map is highly reliable and useful for a variety of genomics studies.

Construction and characterization of a bacterial artificial chromosome library for Camellia sinensis

2017 ◽  
Vol 13 (4) ◽  
Author(s):  
Yuling Tai ◽  
Hao Wang ◽  
Chaoling Wei ◽  
Ling Su ◽  
Min Li ◽  
...  
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Camellia Sinensis ◽  
Bacterial Artificial Chromosome Library ◽  
Artificial Chromosome

scholarly journals Physical map of theAzoarcussp. strain BH72 genome based on a bacterial artificial chromosome library as a platform for genome sequencing and functional analysis

2005 ◽  
Vol 249 (2) ◽  
pp. 233-240 ◽  
Author(s):  
Federico Battistoni ◽  
Daniela Bartels ◽  
Olaf Kaiser ◽  
Stella Marie Reamon-Buettner ◽  
Thomas Hurek ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Bacterial Artificial Chromosome ◽  
Genome Sequencing ◽  
Bacterial Artificial Chromosome Library ◽  
Physical Map ◽  
Artificial Chromosome

A physical map with yeast artificial chromosome (YAC) clones covering 63% of the 12 rice chromosomes

Genome ◽  
2001 ◽  
Vol 44 (1) ◽  
pp. 32-37 ◽  
Author(s):  
Shoko Saji ◽  
Yosuke Umehara ◽  
Baltazar A Antonio ◽  
Hiroko Yamane ◽  
Hiroshi Tanoue ◽  
...  
Keyword(s):  
Genetic Map ◽  
Dna Markers ◽  
Yeast Artificial Chromosome ◽  
Physical Map ◽  
Genome Structure ◽  
Rice Genome ◽  
Artificial Chromosome ◽  
Physical Length ◽  
Rice Chromosomes ◽  
Yac Contig

A new YAC (yeast artificial chromosome) physical map of the 12 rice chromosomes was constructed utilizing the latest molecular linkage map. The 1439 DNA markers on the rice genetic map selected a total of 1892 YACs from a YAC library. A total of 675 distinct YACs were assigned to specific chromosomal locations. In all chromosomes, 297 YAC contigs and 142 YAC islands were formed. The total physical length of these contigs and islands was estimated to 270 Mb which corresponds to approximately 63% of the entire rice genome (430 Mb). Because the physical length of each YAC contig has been measured, we could then estimate the physical distance between genetic markers more precisely than previously. In the course of constructing the new physical map, the DNA markers mapped at 0.0-cM intervals were ordered accurately and the presence of potentially duplicated regions among the chromosomes was detected. The physical map combined with the genetic map will form the basis for elucidation of the rice genome structure, map-based cloning of agronomically important genes, and genome sequencing.Key words: physical mapping, YAC contig, rice genome, rice chromosomes.

scholarly journals Characterization of bacterial artificial chromosome transgenic mice expressing mCherry fluorescent protein substituted for the murine smooth muscle α-actin gene

genesis ◽  
2010 ◽  
Vol 48 (7) ◽  
pp. 457-463 ◽  
Author(s):  
John J. Armstrong ◽  
Irina V. Larina ◽  
Mary E. Dickinson ◽  
Warren E. Zimmer ◽  
Karen K. Hirschi
Keyword(s):  
Smooth Muscle ◽  
Bacterial Artificial Chromosome ◽  
Transgenic Mice ◽  
Fluorescent Protein ◽  
Artificial Chromosome ◽  
Actin Gene

The genetic and RFLP characterization of the left end of linkage group III in Caenorhabditis elegans

Genome ◽  
1993 ◽  
Vol 36 (4) ◽  
pp. 712-724 ◽  
Author(s):  
Dave Pilgrim
Keyword(s):  
Caenorhabditis Elegans ◽  
Linkage Group ◽  
Genetic Map ◽  
Physical Map ◽  
Group Iii ◽  
C Elegans ◽  
Genomic Regions ◽  
Caenorhabditis Elegans Genome ◽  
Selection Of

A genetic approach was taken to identify new transposable element Tc1 -dependent polymorphisms on the left end of linkage group III in the nematode Caenorhabditis elegans. The cloning of the genomic DNA surrounding the Tc1 allowed the selection of overlapping clones (from the collection being used to assemble the physical map of the C. elegans genome). A contig of approximately 600–800 kbp in the region has been identified, the genetic map of the region has been refined, and 10 new RFLPs as well as at least four previously characterized genetic loci have been positioned onto the physical map, to the resolution of a few cosmids. This analysis demonstrated the ability to combine physical and genetic mapping for the rapid analysis of large genomic regions (0.5–1 Mbp) in genetically amenable eukaryotes.Key words: Caenorhabditis elegans, genome analysis, RFLP, physical map, genetic map.

Construction and characterization of two rice bacterial artificial chromosome libraries from the parents of a permanent recombinant inbred mapping population

1996 ◽  
Vol 2 (1) ◽  
Author(s):  
Hong-Bin Zhang ◽  
Sangdun Choi ◽  
Sung-Sick Woo ◽  
Zhikang Li ◽  
RodA. Wing
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Recombinant Inbred ◽  
Mapping Population ◽  
Artificial Chromosome

Construction and characterization of a bacterial artificial chromosome library ofArabidopsis thaliana

1995 ◽  
Vol 13 (2) ◽  
pp. 124-128 ◽  
Author(s):  
Sangdun Choi ◽  
Robert A. Creelman ◽  
John E. Mullet ◽  
Rod A. Wing
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Bacterial Artificial Chromosome Library ◽  
Artificial Chromosome ◽  
Ofarabidopsis Thaliana
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