scholarly journals Expression of Escherichia coli Glycogen Synthase in the Tubers of Transgenic Potatoes (Solanum tuberosum) Results in a Highly Branched Starch

1994 ◽  
Vol 104 (4) ◽  
pp. 1159-1166 ◽  
Author(s):  
C. K. Shewmaker ◽  
C. D. Boyer ◽  
D. P. Wiesenborn ◽  
D. B. Thompson ◽  
M. R. Boersig ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Solanum Tuberosum ◽  
Glycogen Synthase ◽  
Transgenic Potatoes

De Novo synthesis of Escherichia coli glycogen is due to primer associated with glycogen synthase and activation by branching enzyme

1978 ◽  
Vol 190 (2) ◽  
pp. 385-397 ◽  
Author(s):  
Kichitaro Kawaguchi ◽  
Jeffrey Fox ◽  
Eric Holmes ◽  
Charles Boyer ◽  
Jack Preiss
Keyword(s):  
Escherichia Coli ◽  
De Novo ◽  
Glycogen Synthase ◽  
Branching Enzyme ◽  
De Novo Synthesis

Functional analysis of a class I patatin gene SK24-1 in microtuber formation of transgenic potatoes

2008 ◽  
Vol 88 (4) ◽  
pp. 593-598 ◽  
Author(s):  
Huaijun Si ◽  
Jun Liu ◽  
Jian Huang ◽  
Conghua Xie
Keyword(s):  
Escherichia Coli ◽  
Cdna Clone ◽  
Antisense Rna ◽  
Cauliflower Mosaic Virus ◽  
Class I ◽  
35S Promoter ◽  
Transformed Plants ◽  
Transgenic Potatoes ◽  
Class I Patatin

Expression of a class I patatin cDNA clone, SK24-1, in Escherichia coli revealed that the cDNA clone possessed lipid acyl hydrolase (LAH) activity. Transformed potato plants were obtained via Agrobacterium-mediated transformation using the chimeric constructs containing the sense and antisense cDNA under the control cauliflower mosaic virus 35S (CaMV 35S) promoter. In some sense transformed plants, both sense patatin RNA and LAH activity were increased and further resulted in a significant increase of percentage of plantlets that formed microtubers and numbers of microtubers per plantlet in vitro. All antisense plants displayed a reduction in LAH activity. Both sense and antisense RNA could be detected in antisense plants, but transcripts of antisense RNA resulted in a reduction of endogenous sense RNA. Moreover, expression of antisense cDNA in some antisense transformed plants led to a significant decrease in the number of microtubers formed. These results suggest that SK24-1 was involved in regulating microtuber formation. Key words: Patatin, potato, Escherichia coli, sense RNA, antisense RNA

Properties of a glycogen like polysaccharide produced by a mutant of Escherichia coli lacking glycogen synthase and maltodextrin phosphorylase

2016 ◽  
Vol 136 ◽  
pp. 649-655 ◽  
Author(s):  
Ji-Yun Kwak ◽  
Min-Gyu Kim ◽  
Young-Wan Kim ◽  
Hyun-Seung Ban ◽  
Mi-Sun Won ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Glycogen Synthase ◽  
Maltodextrin Phosphorylase

The ADP-glucose binding site of the Escherichia coli glycogen synthase

2006 ◽  
Vol 453 (2) ◽  
pp. 188-196 ◽  
Author(s):  
Alejandra Yep ◽  
Miguel A. Ballicora ◽  
Jack Preiss
Keyword(s):  
Escherichia Coli ◽  
Binding Site ◽  
Glycogen Synthase ◽  
Glucose Binding

Expression and chloroplast-targeting of active phosphoenolpyruvate synthetase from Escherichia coli in Solanum tuberosum

Plant Science ◽  
1997 ◽  
Vol 127 (2) ◽  
pp. 191-205 ◽  
Author(s):  
Ralph Panstruga ◽  
Sigrun Hippe-Sanwald ◽  
Yeon-Kyeong Lee ◽  
Marco Lataster ◽  
Volker Lipka ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Solanum Tuberosum ◽  
Chloroplast Targeting

Expression of hybrid cry3aM–licBM2 genes in transgenic potatoes (Solanum tuberosum)

2007 ◽  
Vol 92 (3) ◽  
pp. 321-325 ◽  
Author(s):  
Gholamreza Salehi Jouzani ◽  
Irina V. Goldenkova ◽  
Eleonora S. Piruzian
Keyword(s):  
Solanum Tuberosum ◽  
Transgenic Potatoes

scholarly journals Biofilm Formation and Dispersal under the Influence of the Global Regulator CsrA of Escherichia coli

2002 ◽  
Vol 184 (1) ◽  
pp. 290-301 ◽  
Author(s):  
Debra W. Jackson ◽  
Kazushi Suzuki ◽  
Lawrence Oakford ◽  
Jerry W. Simecka ◽  
Mark E. Hart ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Biofilm Formation ◽  
Rna Binding ◽  
Glycogen Synthase ◽  
Regulatory Protein ◽  
Ectopic Expression ◽  
Knockout Mutant ◽  
E Coli ◽  
K 12 ◽  
Biofilm Development

ABSTRACT The predominant mode of growth of bacteria in the environment is within sessile, matrix-enclosed communities known as biofilms. Biofilms often complicate chronic and difficult-to-treat infections by protecting bacteria from the immune system, decreasing antibiotic efficacy, and dispersing planktonic cells to distant body sites. While the biology of bacterial biofilms has become a major focus of microbial research, the regulatory mechanisms of biofilm development remain poorly defined and those of dispersal are unknown. Here we establish that the RNA binding global regulatory protein CsrA (carbon storage regulator) of Escherichia coli K-12 serves as both a repressor of biofilm formation and an activator of biofilm dispersal under a variety of culture conditions. Ectopic expression of the E. coli K-12 csrA gene repressed biofilm formation by related bacterial pathogens. A csrA knockout mutation enhanced biofilm formation in E. coli strains that were defective for extracellular, surface, or regulatory factors previously implicated in biofilm formation. In contrast, this csrA mutation did not affect biofilm formation by a glgA (glycogen synthase) knockout mutant. Complementation studies with glg genes provided further genetic evidence that the effects of CsrA on biofilm formation are mediated largely through the regulation of intracellular glycogen biosynthesis and catabolism. Finally, the expression of a chromosomally encoded csrA′-′lacZ translational fusion was dynamically regulated during biofilm formation in a pattern consistent with its role as a repressor. We propose that global regulation of central carbon flux by CsrA is an extremely important feature of E. coli biofilm development.

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