scholarly journals Stimulation of Phospholipid Biosynthesis during Frost Hardening of Winter Wheat

1975 ◽  
Vol 55 (2) ◽  
pp. 356-359 ◽  
Author(s):  
Claude Willemot

1988 ◽  
Vol 253 (3) ◽  
pp. 659-665 ◽  
Author(s):  
B E Miller ◽  
G E R Hook

The effects of intratracheally instilled silica (10 mg/rat) on the biosynthesis of surfactant phospholipids was investigated in the lungs of rats. The sizes of the intracellular and extracellular pools of surfactant phospholipids were measured 7, 14 and 28 days after silica exposure. The ability of lung slices to incorporate [14C]choline and [3H]palmitate into surfactant phosphatidylcholine (PC) and disaturated phosphatidylcholine (DSPC) was also investigated. Both intra- and extra-cellular pools of surfactant phospholipids were increased by silica treatment. The intracellular pool increased linearly over the 28-day time period, ultimately reaching a size 62-fold greater than controls. The extracellular pool also increased, but showed a pattern different from that of the intracellular pool. The extracellular pool increased non-linearly up to 14 days, and then declined. At its maximum, the extracellular pool was increased 16-fold over the control. The ability of lung slices to incorporate phospholipid precursors into surfactant-associated PC and DSPC was elevated at all time periods. The rate of incorporation of [14C]choline into surfactant PC and DSPC was maximal at 14 days and was nearly 3-fold greater than the rate in controls. The rate of incorporation of [3H]palmitate was also maximal at 14 days, approx. 5-fold above controls for PC and 3-fold for DSPC. At this same time point, the microsomal activity of cholinephosphate cytidylyltransferase was increased 4.5-fold above controls, but cytosolic activity was not significantly affected by silica treatment. These data indicate that biosynthesis of surfactant PC is elevated after treatment of lungs with silica and that this increased biosynthesis probably underlies the expansion of the intra- and extra-cellular pools of surfactant phospholipids.





2013 ◽  
Vol 38 (1) ◽  
pp. 5-10 ◽  
Author(s):  
Aleksander Brzóstowicz ◽  
Zdzisław Prokowski ◽  
Antoni Murkowski ◽  
Edward Grabikowski

The influence of frost hardening conditions on the frost-resistance of three winter wheat cultivars ('Mironowska 808', 'Grana' and 'Maris Huntsman') was studied. Frost resistance was measured periodically during the autumn and winter season by the delayed luminescence method. Parallelly an electrical conductivity test was performed for comparison. It was found that the temperature which delayed luminescence reaches its maximum (t<sub>N</sub>) correlates with (t<sub>50</sub>) obtained by the conductometric method. The differences in response of particular cultivars to frost and hardening conditions are described.



1986 ◽  
Vol 34 (4) ◽  
pp. 488-493
Author(s):  
H.J.W. van Roermund ◽  
J.R.R. Groot ◽  
W.A.H. Rossing ◽  
R. Rabbinge
Keyword(s):  


1979 ◽  
Vol 59 (3) ◽  
pp. 639-643 ◽  
Author(s):  
C. WILLEMOT ◽  
L. PELLETIER

Low soil moisture, both prior to and during freezing, increased frost resistance of 12-day-old winter wheat (Triticum aestivum L.) plants. While frost resistance increased, percentage of linolenic acid decreased in drought-stressed plants (10% of soil water-holding capacity) as compared with controls (40%). This occurred whether drought stress was applied before freezing at 1 °C or at 20 °C. A degree of frost hardening can be achieved in winter wheat by drought stress without low temperature treatment, and without an increase in degree of unsaturation of fatty acids.



2002 ◽  
Vol 24 (3) ◽  
pp. 291-295 ◽  
Author(s):  
Anna Janeczko ◽  
Władysław Filek


Cryobiology ◽  
1977 ◽  
Vol 14 (1) ◽  
pp. 87-93 ◽  
Author(s):  
C. Willemot ◽  
H.J. Hope ◽  
R.J. Williams ◽  
R. Michaud


Cryobiology ◽  
1975 ◽  
Vol 12 (6) ◽  
pp. 566-567
Author(s):  
C. Willemot ◽  
H.J. Hope ◽  
R.J. Williams


1986 ◽  
Vol 28 (5) ◽  
pp. 396-398
Author(s):  
Daniela Zemlová


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