The effect of sodium humate on the content of different types of phosphorus compounds and on the activity of acid phosphatases in tomato seedlings

The effect of sodium humate on the level of some fractions of phosphorus compounds in the leaves and roots of tomatoes cultured in mediums with a full or reduced phosphorus dose was studied. A dependence was found between the presence of humate in the medium and an increase in the amount of some organic phosphorus compounds in the leaves (lipid phosphorus, easily hydrolyzed phosphorus compounds, nucleic acid phosphorus). In addition, humate lowered the activity of acid phosphatases which had been excessively increased due to the plants inadequate phosphate supply. The results suggest that Na-humate affects phosphorus metabolism not only in an indirect manner, but also directly through penetration of humic substances into the plant tissues.

Study on BAF Lipid Phosphorus Biomass and SOUR Active Tests

By taking the fillings from the wastewater treatment reactor of a practically stable operation of the BAF, it is to test the lipid phosphorus biomass both in the bio-film and floc in the fillings, and active changing laws of microbes. The experiments show that BAF sewage treatment results from the joint action of bio-film and biological floc. The experiments provide theoretical foundations for the operating mechanism of BAF, packing height and the optimization of backwash time. Due to its high efficiency, energy saving, small area, easy operation and management, Biological Aerated Filter (BAF) technology has become a research focus[1] of a biological wastewater treatment technology in recent years. BAF process is characterized of a lot of filler in the activated sludge, besides the bio-film on the fillings. This sludge contains active living organisms, shed bio-film, suspended matters from the raw sewage and other adsorptions from the wastewater. They are collectively referred to as biological floc. When oxidating and decomposing organic matters in the sewage, it intercepts and adsorb sunken substances. Throughout BAF operation process, it is as irreplaceable as the bio-film.

Binding of doxorubicin to cardiolipin as compared to other anionic phospholipids—An evaluation of electrostatic effects

The binding of doxorubicin to large unilamellar vesicles consisting of cardiolipin or other anionic phospholipids was analyzed in terms of the local drug concentration at the membrane surface, according to the Gouy-Chapman theory. The analysis suggests strong positive binding cooperativity. Part of the drug binds in the uncharged form. The affinity for cardiolipin and other anionic phospholipids is comparable. A binding level of 0.5 doxorubicin per lipid-phosphorus is reached when the local concentration of free doxorubicin monomer-equivalents at the membrane surface is about 0.2–0.7 mM. This contrasts with earlier findings indicating a 300–1000 fold higher affinity for cardiolipin. The present analysis provides an explanation for this apparent discrepancy.

MICROSOMAL MEMBRANE CHANGES IN IRRADIATED CAULIFLOWER DURING STORAGE

Cauliflowers (Brassica oleracea) were irradiated at 0, 2, and 4 kGy and stored 8 days at 13°C. Development of yellow color and browning of the in florescence, increase in membrane electrolyte leakage and reduction of protein recovery in microsomal membranes were observed over the storage period. Changes in membrane free fatty acids, lipid phosphorus content, peroxydation level, and fatty acid composition of polar lipids also occurred. These results indicate an important modification of cellular membranes. The direct effect of gamma rays on membrane lipids via free radical production and subsequent destabilization of the lipid bilayer during storage could be responsable for earlier onset of senescence.