scholarly journals Time Course and Spatial Distribution of Phenylalanine Ammonia-Lyase and Peroxidase Activity in Wounded Potato Tuber Tissue

1978 ◽  
Vol 62 (5) ◽  
pp. 789-793 ◽  
Author(s):  
Rolf Borchert
2000 ◽  
Vol 55 (5-6) ◽  
pp. 373-382 ◽  
Author(s):  
Fumio Matsuda ◽  
Hisashi Miyagawa ◽  
Tamio Ueno

Potato tuber disks, when treated with laminarin, a β-1,3-glucooligosaccharide from Laminaria digitata, accumulate a hydroxycinnamoyl amide compound, N-p-coumaroyloctopamine (p-CO). The biosynthesis of p -CO was investigated by feeding experiments, in order to show that the precursors of N-p-coumaroyl and octopamine moieties of p-CO are ʟ-phenylalanine and ʟ-tyrosine, respectively. The treatment of potato tuber tissue with laminarin resulted in elevated activities of four enzymes which are putatively involved in p-CO biosynthesis: phenylalanine ammonia lyase (PAL; EC 4.3.1.5), 4-hydroxycinnamic acid:CoA ligase (4CL; EC 6.2.1.12), hydroxycinnamoyl-CoA:tyramine N-(hydroxycinnamoyl)transferase (THT; EC 2.3.1.110) and tyrosine decarboxylase (TyrDC; EC 4.1.1.25). Among these, the response of TyrDC was specific to laminarin treatment, thus indicating that the regulation of TyrDC activity is critical for the accumulation of p -CO in potato tuber tissue.


1969 ◽  
Vol 46 (7) ◽  
pp. 227-233
Author(s):  
John F. Kelly ◽  
Howard L. Adams ◽  
Charles E. Cunningham

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