scholarly journals Peptidoglycan binding by a pocket on the accessory NTF2-domain of Pgp2 directs the helical cell shape of Campylobacter jejuni

2021 ◽  
Vol 77 (a1) ◽  
pp. a183-a183
Author(s):  
Chang Sheng-Huei Lin ◽  
Anson Chan ◽  
Jenny Vermeulen ◽  
Jacob Brockerman ◽  
Arvind Soni ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Cell Shape ◽  
Ntf2 Domain

scholarly journals Peptidoglycan binding by a pocket on the accessory NTF2-domain of Pgp2 directs helical cell shape of Campylobacter jejuni

2021 ◽  
Vol 296 ◽  
pp. 100528
Author(s):  
Chang Sheng-Huei Lin ◽  
Anson C.K. Chan ◽  
Jenny Vermeulen ◽  
Jacob Brockerman ◽  
Arvind S. Soni ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Cell Shape ◽  
Ntf2 Domain

scholarly journals Identification and initial characterisation of a protein involved in Campylobacter jejuni cell shape

2017 ◽  
Vol 104 ◽  
pp. 202-211 ◽  
Author(s):  
Diane Esson ◽  
Srishti Gupta ◽  
David Bailey ◽  
Paul Wigley ◽  
Amy Wedley ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Cell Shape

Bent Bacteria: A Comparison of Cell Shape Mechanisms inProteobacteria

2019 ◽  
Vol 73 (1) ◽  
pp. 457-480 ◽  
Author(s):  
Jennifer A. Taylor ◽  
Sophie R. Sichel ◽  
Nina R. Salama
Keyword(s):  
Helicobacter Pylori ◽  
Gastrointestinal Tract ◽  
Phylogenetic Tree ◽  
Vibrio Cholerae ◽  
Campylobacter Jejuni ◽  
Functional Significance ◽  
Cell Shape ◽  
Caulobacter Crescentus ◽  
Selective Pressures

Helical cell shape appears throughout the bacterial phylogenetic tree. Recent exciting work characterizing cell shape mutants in a number of curved and helical Proteobacteria is beginning to suggest possible mechanisms and provide tools to assess functional significance. We focus here on Caulobacter crescentus, Vibrio cholerae, Helicobacter pylori, and Campylobacter jejuni, organisms from three classes of Proteobacteria that live in diverse environments, from freshwater and saltwater to distinct compartments within the gastrointestinal tract of humans and birds. Comparisons among these bacteria reveal common themes as well as unique solutions to the task of maintaining cell curvature. While motility appears to be influenced in all these bacteria when cell shape is perturbed, consequences on niche colonization are diverse, suggesting the need to consider additional selective pressures.

scholarly journals Peptidoglycan-Modifying Enzyme Pgp1 Is Required for Helical Cell Shape and Pathogenicity Traits in Campylobacter jejuni

2012 ◽  
Vol 8 (3) ◽  
pp. e1002602 ◽  
Author(s):  
Emilisa Frirdich ◽  
Jacob Biboy ◽  
Calvin Adams ◽  
Jooeun Lee ◽  
Jeremy Ellermeier ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Cell Shape

scholarly journals “Campylobacter jejuni motility integrates specialized cell shape, flagellar filament, and motor, to coordinate action of its opposed flagella in viscous media”

2019 ◽  
Author(s):  
Eli J. Cohen ◽  
Daisuke Nakane ◽  
Yoshiki Kabata ◽  
David R. Hendrixson ◽  
Takayuki Nishizaka ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Cell Body ◽  
Cell Shape ◽  
High Speed ◽  
High Viscosity ◽  
High Speed Video ◽  
Flagellar Motors ◽  
Flagellar Filament ◽  
Viscous Media ◽  
Cell Plan

AbstractCampylobacter jejuni rotates a flagellum at each pole to swim through the viscous mucosa of its hosts’ gastrointestinal tracts. Despite their importance for host colonization, however, how C. jejuni coordinates rotation of these two opposing flagella is unclear. As well as their polar placement, C. jejuni’s flagella deviate from the Enterobacteriaceael norm in other ways: their flagellar motors produce much higher torque and their flagellar filament is made of two different zones of two different flagellins. To understand how C. jejuni’s opposed motors coordinate, and what contribution these factors play in C. jejuni motility, we developed strains with flagella that could be fluorescently labeled, and observed them by high-speed video microscopy. We found that C. jejuni coordinates its dual flagella by wrapping the leading filament around the cell body during swimming in high-viscosity media and that its differentiated flagellar filament has evolved to facilitate this wrapped-mode swimming. Unexpectedly, C. jejuni’s helical body is important for facile unwrapping of the flagellar filament from the cell body during switching of swimming trajectory. Our findings demonstrate how multiple facets of C. jejuni’s flagella and cell plan have co-evolved for optimal motility in high-viscosity environments.

scholarly journals Pgp2 is an LD-carboxypeptidase that determines the helical cell shape of Campylobacter jejuni

2018 ◽  
Vol 74 (a1) ◽  
pp. a128-a128
Author(s):  
Chang Sheng-Huei Lin ◽  
Anson C. K. Chan ◽  
Jenny Vermeulen ◽  
Erin C. Gaynor ◽  
Michael E. P. Murphy
Keyword(s):  
Campylobacter Jejuni ◽  
Cell Shape

scholarly journals Campylobacter jejuni motility integrates specialized cell shape, flagellar filament, and motor, to coordinate action of its opposed flagella

2020 ◽  
Vol 16 (7) ◽  
pp. e1008620 ◽  
Author(s):  
Eli J. Cohen ◽  
Daisuke Nakane ◽  
Yoshiki Kabata ◽  
David R. Hendrixson ◽  
Takayuki Nishizaka ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Cell Shape ◽  
Specialized Cell ◽  
Flagellar Filament

scholarly journals Peptidoglycan reshaping by a noncanonical peptidase for helical cell shape in Campylobacter jejuni

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Kyungjin Min ◽  
Doo Ri An ◽  
Hye-Jin Yoon ◽  
Neha Rana ◽  
Ji Su Park ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Cell Shape

scholarly journals Correction: Peptidoglycan-Modifying Enzyme Pgp1 Is Required for Helical Cell Shape and Pathogenicity Traits in Campylobacter jejuni

2012 ◽  
Vol 8 (10) ◽  
Author(s):  
Emilisa Frirdich ◽  
Jacob Biboy ◽  
Calvin Adams ◽  
Jooeun Lee ◽  
Jeremy Ellermeier ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Cell Shape

Modulated polarization microscopy displays the dynamics of cytoskeletal structures in living, motile cells

1995 ◽  
Vol 53 ◽  
pp. 902-903
Author(s):  
J. R. Kuhn ◽  
M. Poenie
Keyword(s):  
Mitotic Spindle ◽  
Actin Filaments ◽  
Cell Shape ◽  
Dynamic Structure ◽  
Living Cells ◽  
Cytoskeletal Proteins ◽  
Polarization Microscopy ◽  
Video Enhancement ◽  
Ultrastructural Studies ◽  
Motile Cells

Cell shape and movement are controlled by elements of the cytoskeleton including actin filaments an microtubules. Unfortunately, it is difficult to visualize the cytoskeleton in living cells and hence follow it dynamics. Immunofluorescence and ultrastructural studies of fixed cells while providing clear images of the cytoskeleton, give only a static picture of this dynamic structure. Microinjection of fluorescently Is beled cytoskeletal proteins has proved useful as a way to follow some cytoskeletal events, but long terry studies are generally limited by the bleaching of fluorophores and presence of unassembled monomers.Polarization microscopy has the potential for visualizing the cytoskeleton. Although at present, it ha mainly been used for visualizing the mitotic spindle. Polarization microscopy is attractive in that it pro vides a way to selectively image structures such as cytoskeletal filaments that are birefringent. By combing ing standard polarization microscopy with video enhancement techniques it has been possible to image single filaments. In this case, however, filament intensity depends on the orientation of the polarizer and analyzer with respect to the specimen.

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