scholarly journals Crystallization of Spätzle, a cystine-knot protein involved in embryonic development and innate immunity inDrosophila melanogaster

2008 ◽  
Vol 64 (8) ◽  
pp. 707-710 ◽  
Author(s):  
Anita Hoffmann ◽  
Piotr Neumann ◽  
Angelika Schierhorn ◽  
Milton T. Stubbs
Keyword(s):  
Innate Immunity ◽  
Embryonic Development ◽  
Cystine Knot ◽  
Cystine Knot Protein

High level expression of the Drosophila Toll receptor ectodomain and crystallization of its complex with the morphogen Spätzle

2013 ◽  
Vol 394 (8) ◽  
pp. 1091-1096 ◽  
Author(s):  
Marco Stelter ◽  
Uwe Fandrich ◽  
Kati Franzke ◽  
Angelika Schierhorn ◽  
Constanze Breithaupt ◽  
...  
Keyword(s):  
Immune Response ◽  
High Level Expression ◽  
Receptor Ligand ◽  
High Affinity ◽  
Cystine Knot ◽  
Toll Receptor ◽  
Cystine Knot Protein ◽  
High Yields ◽  
High Level ◽  
Affinity Interaction

Abstract Drosophila Toll receptors are involved in embryonic development and in the immune response of adult flies. In both processes, the Toll receptor ligand is the NGF-like cystine knot protein Spätzle. Here we present the expression of Toll receptor ectodomain in Schneider cells at high yields and demonstrate a high affinity interaction with the refolded and trypsin-processed Spätzle cystine knot domain dimer. Poorly and anisotropically diffracting crystals of the complex could be improved by deglycosylation and dehydration, paving the way for structural analyses of the Toll-Spätzle interaction.

Structural basis of BMP signalling inhibition by the cystine knot protein Noggin

Nature ◽  
2002 ◽  
Vol 420 (6916) ◽  
pp. 636-642 ◽  
Author(s):  
Jay Groppe ◽  
Jason Greenwald ◽  
Ezra Wiater ◽  
Joaquin Rodriguez-Leon ◽  
Aris N. Economides ◽  
...  
Keyword(s):  
Structural Basis ◽  
Cystine Knot ◽  
Cystine Knot Protein ◽  
Bmp Signalling

scholarly journals Selecting highly structure-specific antibodies using structured synthetic mimics of the cystine knot protein sclerostin

2012 ◽  
Vol 25 (5) ◽  
pp. 251-259 ◽  
Author(s):  
J. W. Back ◽  
C. Frisch ◽  
K. Van Pee ◽  
V. Boschert ◽  
R. van Vught ◽  
...  
Keyword(s):  
Specific Antibodies ◽  
Cystine Knot ◽  
Cystine Knot Protein

The race-specific elicitor AVR9 of the tomato pathogen Cladosporium fulvum : a cystine knot protein

FEBS Letters ◽  
1997 ◽  
Vol 404 (2-3) ◽  
pp. 153-158 ◽  
Author(s):  
Jacques Vervoort ◽  
Henno W van den Hooven ◽  
Axel Berg ◽  
Paul Vossen ◽  
Ralph Vogelsang ◽  
...  
Keyword(s):  
Cladosporium Fulvum ◽  
Cystine Knot ◽  
Cystine Knot Protein ◽  
Tomato Pathogen

scholarly journals The Structure of a Two-Disulfide Intermediate Assists in Elucidating the Oxidative Folding Pathway of a Cyclic Cystine Knot Protein

Structure ◽  
2008 ◽  
Vol 16 (6) ◽  
pp. 842-851 ◽  
Author(s):  
Maša Cemazar ◽  
Ajinkya Joshi ◽  
Norelle L. Daly ◽  
Alan E. Mark ◽  
David J. Craik
Keyword(s):  
Folding Pathway ◽  
Oxidative Folding ◽  
Cystine Knot ◽  
Cystine Knot Protein

STRUCTURAL BASIS OF BMP SIGNALING INHIBITION BY NOGGIN, A NOVEL TWELVE-MEMBERED CYSTINE KNOT PROTEIN

2003 ◽  
Vol 85 ◽  
pp. 52-58 ◽  
Author(s):  
JAY GROPPE ◽  
JASON GREENWALD ◽  
EZRA WIATER ◽  
JOAQUIN RODRIGUEZ-LEON ◽  
ARIS N. ECONOMIDES ◽  
...  
Keyword(s):  
Bmp Signaling ◽  
Structural Basis ◽  
Cystine Knot ◽  
Cystine Knot Protein

scholarly journals Biophysical Characterization of RefoldedDrosophilaSpätzle, a Cystine Knot Protein, Reveals Distinct Properties of Three Isoforms

2008 ◽  
Vol 283 (47) ◽  
pp. 32598-32609 ◽  
Author(s):  
Anita Hoffmann ◽  
Andreas Funkner ◽  
Piotr Neumann ◽  
Susanne Juhnke ◽  
Matthias Walther ◽  
...  
Keyword(s):  
Biophysical Characterization ◽  
Cystine Knot ◽  
Cystine Knot Protein
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