scholarly journals Crystallization and X-ray diffraction analysis of the RNA primer/promoter-binding domain of influenza A virus RNA-dependent RNA polymerase PB2

2009 ◽  
Vol 65 (2) ◽  
pp. 144-146 ◽  
Author(s):  
Takashi Kuzuhara ◽  
Daisuke Kise ◽  
Hiroko Yoshida ◽  
Takahiro Horita ◽  
Yoshimi Murazaki ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Diffraction Analysis ◽  
Influenza A Virus ◽  
Influenza A ◽  
Binding Domain ◽  
X Ray Diffraction ◽  
Rna Dependent Rna Polymerase ◽  
X Ray ◽  
Virus Rna

scholarly journals Structural Basis of the Influenza A Virus RNA Polymerase PB2 RNA-binding Domain Containing the Pathogenicity-determinant Lysine 627 Residue

2009 ◽  
Vol 284 (11) ◽  
pp. 6855-6860 ◽  
Author(s):  
Takashi Kuzuhara ◽  
Daisuke Kise ◽  
Hiroko Yoshida ◽  
Takahiro Horita ◽  
Yoshimi Murazaki ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Influenza A Virus ◽  
Influenza A ◽  
Rna Binding ◽  
Binding Domain ◽  
Structural Basis ◽  
Virus Rna ◽  
Pathogenicity Determinant ◽  
Rna Binding Domain

scholarly journals A Parallel Phenotypic Versus Target-Based Screening Strategy for RNA-Dependent RNA Polymerase Inhibitors of the Influenza A Virus

Viruses ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 826 ◽  
Author(s):  
Xiujuan Zhao ◽  
Yanyan Wang ◽  
Qinghua Cui ◽  
Ping Li ◽  
Lin Wang ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Influenza A Virus ◽  
Influenza A ◽  
Screening Strategy ◽  
Control Analysis ◽  
Virus Infections ◽  
Rdrp Activity ◽  
Rna Dependent Rna Polymerase ◽  
Virus Rna ◽  
Promising Target

Influenza A virus infections cause significant morbidity and mortality, and novel antivirals are urgently needed. Influenza RNA-dependent RNA polymerase (RdRp) activity has been acknowledged as a promising target for novel antivirals. In this study, a phenotypic versus target-based screening strategy was established to identify the influenza A virus inhibitors targeting the virus RNA transcription/replication steps by sequentially using an RdRp-targeted screen and a replication-competent reporter virus-based approach using the same compounds. To demonstrate the utility of this approach, a pilot screen of a library of 891 compounds derived from natural products was carried out. Quality control analysis indicates that the primary screen was robust for identification of influenza A virus inhibitors targeting RdRp activity. Finally, two hit candidates were identified, and one was validated as a putative RdRp inhibitor. This strategy can greatly reduce the number of false positives and improve the accuracy and efficacy of primary screening, thereby providing a powerful tool for antiviral discovery.

Influenza A virus RNA-dependent RNA polymerase cleaves influenza mRNA in vitro

1996 ◽  
Vol 42 (1-2) ◽  
pp. 149-158 ◽  
Author(s):  
Qinghai Peng ◽  
Jose M. Galarza ◽  
Licheng Shi ◽  
Donald F. Summers
Keyword(s):  
Rna Polymerase ◽  
Influenza A Virus ◽  
Influenza A ◽  
Rna Dependent Rna Polymerase ◽  
Virus Rna
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