Crystallization and preliminary X-ray diffraction analysis of the RNA-dependent RNA polymerase ofThosea asignavirus

While DNA-dependent RNA polymerase represents one of the key enzymes involved in transcription and ultimately in gene expression in procaryotic and eucaryotic cells, little progress has been made towards elucidation of its 3-D structure at the molecular level over the past few years. This is mainly because to date no 3-D crystals suitable for X-ray diffraction analysis have been obtained with this rather large (MW ~500 kd) multi-subunit (α2ββ'ζ). As an alternative, we have been trying to form ordered arrays of RNA polymerase from E. coli suitable for structural analysis in the electron microscope combined with image processing. Here we report about helical polymers induced from holoenzyme (α2ββ'ζ) at low ionic strength with 5-7 mM MnCl2 (see Fig. 1a). The presence of the ζ-subunit (MW 86 kd) is required to form these polymers, since the core enzyme (α2ββ') does fail to assemble into such structures under these conditions.

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Crystallization of Proteins, Nucleic Acids, and Viruses for X‐ray Diffraction Analysis

Biopolymers Online ◽

10.1002/3527600035.bpol8016 ◽

2003 ◽

Cited By ~ 3

Author(s):

Alexander McPherson ◽

Robert Cudney ◽

Sam Patel

Keyword(s):

Nucleic Acids ◽

Diffraction Analysis ◽

X Ray Diffraction ◽

X Ray

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Electrooptical Effects on Thin Organic Films and X-Ray Diffraction Analysis of 3-Nitroaniline and 2-Cyclo-Octylamino-5-Nitropyridine

10.21236/ada379414 ◽

2000 ◽

Author(s):

Alexander Leyderman ◽

M. Antipin

Keyword(s):

Diffraction Analysis ◽

Organic Films ◽

X Ray Diffraction ◽

X Ray ◽

Thin Organic Films

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Ten-Vertex Polyhedral Monocarbaborane Chemistry. The Novel High-Yield Formation of the Ten-Vertex closo Compound [6-(PMe2Ph)-closo-1-CB9H9] from the Thermolysis of [8,8-(PMe2Ph)2-nido-8,7-PtCB9H11]

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19932924 ◽

1993 ◽

Vol 58 (12) ◽

pp. 2924-2935 ◽

Cited By ~ 8

Author(s):

Jane H. Jones ◽

Bohumil Štíbr ◽

John D. Kennedy ◽

Mark Thornton-Pett

Keyword(s):

Nmr Spectroscopy ◽

Diffraction Analysis ◽

High Yield ◽

Monoclinic Space Group ◽

The Novel ◽

X Ray Diffraction ◽

Metal Centre ◽

X Ray ◽

Yield Formation ◽

Boiling Toluene

Thermolysis of [8,8-(PMe2Ph)2-nido-8,7-PtCB9H11] in boiling toluene solution results in an elimination of the platinum centre and cluster closure to give the ten-vertex closo species [6-(PMe2Ph)-closo-1-CB9H9] in 85% yield as a colourles air stable solid. The product is characterized by NMR spectroscopy and single-crystal X-ray diffraction analysis. Crystals (from hexane-dichloromethane) are monoclinic, space group P21/c, with a = 903.20(9), b = 1 481.86(11), c = 2 320.0(2) pm, β = 97.860(7)° and Z = 8, and the structure has been refined to R(Rw) = 0.045(0.051) for 3 281 observed reflections with Fo > 2.0σ(Fo). The clean high-yield elimination of a metal centre from a polyhedral metallaborane or metallaheteroborane species is very rare.

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Bis(μ-carboxylato)dienerhodium(I) Complexes - Synthesis, Characterization and Catalytic Activity

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc20081205 ◽

2008 ◽

Vol 73 (8-9) ◽

pp. 1205-1221 ◽

Cited By ~ 4

Author(s):

Jiří Zedník ◽

Jan Sedláček ◽

Jan Svoboda ◽

Jiří Vohlídal ◽

Dmitrij Bondarev ◽

...

Keyword(s):

Catalytic Activity ◽

Diffraction Analysis ◽

Spectroscopic Methods ◽

X Ray Diffraction ◽

X Ray

Dinuclear rhodium(I) η2:η2-cycloocta-1,5-diene (series a) and η2:η2-norborna-2,5-diene (series b) complexes with μ-RCOO- ligands, where R is linear C21H43 (complexes 1a, 1b), CH2CMe3 (2a, 2b), 1-adamantyl (3a, 3b) and benzyl (4a, 4b), have been prepared and characterized by spectroscopic methods. Structures of complexes 2b, 3a and 4a were determined by X-ray diffraction analysis. Complexes prepared show low to moderate catalytic activity in polymerization of phenylacetylene in THF giving high-cis-transoid polymers, but they show only oligomerization activity in dichloromethane.

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