Structure-function studies of CrmK, an oxidase involved in Caerulomycin A biosynthesis

Caerulomycin A (CRM A) is an immunosupressive agent that has a unique 2,2'-bipyridine core structure. Isolated from a marine-derived Actinoalloteichus cyanogriseus, this natural product exhibits antifungal, anti-amoebic, antitumor, and antimicrobial activities. Its biosynthetic pathway consists of more than 20 enzymes, at least seven of which are putatively involved in post-PKS/NRPS modifications of the scaffold. Among these, CrmK is a flavin-dependent oxidase. We have determined the crystal structure of CrmK bound to its flavin adenin dinucleotide (FAD) cofactor at 1.9 Å resolution. FAD linkage to CrmK is observed via two covalent bonds with protein residues His64 and Cys124. This crystal structure, combined with the activity analysis of both wild-type CrmK and a series of mutants, has revealed the role of active site residues lining the substrate and FAD binding pocket. Our studies add additional molecular insights into the structure and function relationship of the bicovalently flavinylated oxidases.

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Structure and function relationship of phosphatidylglycerol in the stabilization of phosphatidylethanolamine bilayer

Biochemistry ◽

10.1021/bi00445a028 ◽

1989 ◽

Vol 28 (19) ◽

pp. 7708-7712 ◽

Cited By ~ 33

Author(s):

Ana Tari ◽

Leaf Huang

Keyword(s):

Structure And Function ◽

Function Relationship ◽

Structure And Function Relationship ◽

And Function ◽

Relationship Of

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Structure and Function Relationship of Murine Insulin-like Peptide 5 (INSL5): Free C-Terminus Is Essential for RXFP4 Receptor Binding and Activation

Biochemistry ◽

10.1021/bi201093m ◽

2011 ◽

Vol 50 (39) ◽

pp. 8352-8361 ◽

Cited By ~ 36

Author(s):

Alessia Belgi ◽

Mohammed A. Hossain ◽

Fazel Shabanpoor ◽

Linda Chan ◽

Suode Zhang ◽

...

Keyword(s):

Receptor Binding ◽

Structure And Function ◽

C Terminus ◽

Function Relationship ◽

Structure And Function Relationship ◽

And Function ◽

Relationship Of

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Recent Advances in Understanding the Structure and Function Relationship of Multidrug Resistance-Linked ABC Transporter P-glycoprotein

Journal of Cancer Research Updates ◽

10.6000/1929-2279.2016.05.03.2 ◽

2016 ◽

Vol 5 (3) ◽

pp. 88-98 ◽

Cited By ~ 2

Author(s):

Di Xia ◽

Keyword(s):

Multidrug Resistance ◽

Abc Transporter ◽

Structure And Function ◽

P Glycoprotein ◽

Recent Advances ◽

Function Relationship ◽

Structure And Function Relationship ◽

And Function ◽

Relationship Of

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Structure and function relationship of toxin from Chinese scorpion Buthus martensii Karsch (BmKAGAP): Gaining insight into related sites of analgesic activity

Peptides ◽

10.1016/j.peptides.2010.03.017 ◽

2010 ◽

Vol 31 (6) ◽

pp. 995-1000 ◽

Cited By ~ 17

Author(s):

Yong Cui ◽

Gui-Li Guo ◽

Lin Ma ◽

Nan Hu ◽

Yong-Bo Song ◽

...

Keyword(s):

Analgesic Activity ◽

Structure And Function ◽

Function Relationship ◽

Structure And Function Relationship ◽

And Function ◽

Relationship Of ◽

Insight Into ◽

Gaining Insight

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Assessment of cerebrovascular responses to physiological stimuli in identical twins using multimodal imaging and computational fluid dynamics

Journal of Applied Physiology ◽

10.1152/japplphysiol.00348.2020 ◽

2020 ◽

Vol 129 (5) ◽

pp. 1024-1032

Author(s):

Hannah J. Thomas ◽

Usaid Rana ◽

Channa E. Marsh ◽

Harrison T. Caddy ◽

Lachlan J. Kelsey ◽

...

Keyword(s):

Multimodal Imaging ◽

Fluid Dynamic ◽

Monozygotic Twins ◽

Structure And Function ◽

Function Relationship ◽

Structure And Function Relationship ◽

And Function ◽

Genetically Determined ◽

Relationship Of ◽

Computational Fluid Dynamic Modeling

There is acknowledged variability in the Circle of Willis in the general population, yet the structure and function relationship of the cerebrovasculature is poorly understood. Using a combination of magnetic resonance imaging, high-resolution Doppler ultrasound, and computational fluid dynamic modeling, we show that monozygotic twins exhibit differences in cerebrovascular structure and function when exposed to physiological stimuli. These data suggest that the morphology, function, and health of cerebrovascular arteries are not primarily genetically determined.

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Abstract 127: Structure and Function Relationship of Phospholipid Transfer Protein in Lipid Transfer Activity Revealed by Electron Microscopy

Circulation Research ◽

10.1161/res.117.suppl_1.127 ◽

2015 ◽

Vol 117 (suppl_1) ◽

Author(s):

Meng Zhang

Keyword(s):

Electron Microscopy ◽

High Density Lipoproteins ◽

Phospholipid Transfer Protein ◽

Lipid Transfer ◽

Transfer Protein ◽

Phospholipid Transfer ◽

Function Relationship ◽

Structure And Function Relationship ◽

And Function ◽

Relationship Of

Human phospholipid transfer protein (PLTP) mediates the transfer of lipids among atheroprotective high-density lipoproteins (HDL) and atherogenic low-density lipoproteins (LDL) by an unknown mechanism. Delineating this mechanism would be an important step toward the understanding and regulation of PLTP for treating cardiovascular diseases, hypoalphalipoproteinemia and hyperalphalipoproteinemia. Using electron microscopy, negative-staining, and single-particle image processing, we discovered that PLTP penetrates each class of HDL, LDL and liposome independently, and also bridges a ternary complex with one of its distal end-domains penetrating into HDL and another distal domain interacting with LDL. These new insights into PLTP interaction with lipoproteins and liposomes provide a molecular basis for analyzing PLTP-dependent lipid transfer between lipoprotein particles.

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