scholarly journals Crystallization and preliminary X-ray diffraction analysis of nepenthesin-1

2014 ◽  
Vol 70 (a1) ◽  
pp. C446-C446
Author(s):  
Karla Fejfarová ◽  
Petr Man ◽  
Hynek Mrázek ◽  
Alan Kádek ◽  
Petr Halada ◽  
...  
Keyword(s):  
Ministry Of Education ◽  
X Ray Diffraction ◽  
Aspartic Proteases ◽  
Development Fund ◽  
X Ray ◽  
Cell Parameters ◽  
European Regional Development Fund ◽  
Diffusion Technique ◽  
Grant Agency

Carnivorous pitcher plants of the genus Nepenthes secrete their own aspartic proteases, nepenthesins, to digest prey. Nepenthesins differ significantly in sequence from other plant aspartic proteases. This difference, which brings more cysteine residues into the structure of nepenthesins, in conjunction with putative N-glycosylation, can contribute to uniquely high temperature and pH stabilities of these proteases [1, 2]. In continuation of our previous study of the expression and biochemical and enzymatic characterization of a recombinant form of nepenthesin-1 (rNep-1) from Nepenthes gracilis [3], we report its crystallization and preliminary X-ray analysis. Crystals of rNep-1 in complex with the pepstatin A inhibitor have been grown using the hanging-drop vapour-diffusion technique. Diffraction data were collected to 2.9 Å resolution using synchrotron radiation at Bessy II of HZB, Berlin. The crystals belong to space group P21, with unit-cell parameters a = 54.4 Å, b = 86.6 Å, c = 95.8 Å, β = 1060. The self-rotation function combined with solvent-content calculations and Matthews coefficient suggest the presence of two molecules of rNep-1 in the asymmetric unit. This work was supported by the Ministry of Education, Youth and Sports of the Czech Republic (grants No. EE2.3.30.0029 and No. LG14009), by BIOCEV CZ.1.05/1.1.00/02.0109 from the European Regional Development Fund, and by the Grant Agency of the Czech Technical University in Prague, grant No. SGS13/219/OHK4/3T/14.

scholarly journals Crystallization, optimization and preliminary X-ray characterization of a metal-dependent PI-PLC fromStreptomyces antibioticus

2012 ◽  
Vol 68 (11) ◽  
pp. 1378-1386 ◽  
Author(s):  
Michael R. Jackson ◽  
Thomas L. Selby
Keyword(s):  
X Ray Diffraction ◽  
Hanging Drop ◽  
Orthorhombic Space Group ◽  
X Ray ◽  
Streptomyces Antibioticus ◽  
Cell Parameters ◽  
Heavy Atoms ◽  
Hanging Drop Method

A recombinant metal-dependent phosphatidylinositol-specific phospholipase C (PI-PLC) fromStreptomyces antibioticushas been crystallized by the hanging-drop method with and without heavy metals. The native crystals belonged to the orthorhombic space groupP222, with unit-cell parametersa= 41.26,b= 51.86,c = 154.78 Å. The X-ray diffraction results showed significant differences in the crystal quality of samples soaked with heavy atoms. Additionally, drop pinning, which increases the surface area of the drops, was also used to improve crystal growth and quality. The combination of heavy-metal soaks and drop pinning was found to be critical for producing high-quality crystals that diffracted to 1.23 Å resolution.

scholarly journals Growth and Characterization of Pure and Doped L-Alanine Tartrate Single Crystals

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
K. Rajesh ◽  
B. Milton Boaz ◽  
P. Praveen Kumar
Keyword(s):  
Single Crystals ◽  
Vickers Microhardness ◽  
Diffraction Method ◽  
Dielectric Studies ◽  
X Ray Diffraction ◽  
X Ray ◽  
Cell Parameters ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

Single crystals of pure and Lanthanum doped L-Alanine Tartrate were grown by slow evaporation method. The cell parameters were determined using single crystal X-ray diffraction method. To improve the physical properties of the LAT crystal, Lanthanum dopant was added by 2 mol%. ICP studies confirm the presence of Lanthanum in the grown LAT crystal. Transparency range of the crystal was determined using UV-VIS-NIR spectrophotometer. The functional groups of pure and doped LAT crystals were analyzed by FT-IR spectroscopy. Using Vickers microhardness tester, mechanical strength of the material was found. Dielectric studies of pure and doped LAT single crystals were carried out. The doped LAT crystal is found to have efficiency higher than that of pure LAT crystal.

scholarly journals Characterization and structure of glyceraldehyde-3-phosphate dehydrogenase type 1 from Escherichia coli

2020 ◽  
Vol 76 (9) ◽  
pp. 406-413
Author(s):  
L. Zhang ◽  
M. R. Liu ◽  
Y. C. Yao ◽  
I. K. Bostrom ◽  
Y. D. Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Catalytic Domain ◽  
X Ray Diffraction ◽  
Cell Parameters ◽  
Diffusion Technique ◽  
Key Enzyme ◽  
Optimum Reaction ◽  
Biochemical Analyses

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a key enzyme in the glycolytic pathway that catalyzes the conversion of D-glyceraldehyde 3-phosphate to 1,3-diphosphoglycerate. Here, the full-length GAPDH type 1 from Escherichia coli (EcGAPDH1) was cloned and overexpressed, and the protein was purified. Biochemical analyses found that the optimum reaction temperature and pH of EcGAPDH1 were 55°C and 10.0, respectively. The protein has a certain amount of thermostability. Crystals of EcGAPDH1 were obtained using the sitting-drop vapor-diffusion technique and X-ray diffraction data were collected to 1.88 Å resolution. Characterization of the crystals showed that they belonged to space group P41212, with unit-cell parameters a = b = 89.651, c = 341.007 Å, α = β = γ = 90°. The structure of EcGAPDH1 contains four subunits, each of which includes an N-terminal NAD+-binding domain and a C-terminal catalytic domain. Analysis of the NAD+-bound form showed some differences between the structures of EcGAPDH1 and human GAPDH. As EcGAPDH1 shares 100% identity with GAPDH from Shigella sonnei, its structure may help in finding a drug for the treatment of shigellosis.

scholarly journals Purification and X-ray crystallographic analysis of 7-keto-8-aminopelargonic acid (KAPA) synthase fromMycobacterium smegmatis

2014 ◽  
Vol 70 (10) ◽  
pp. 1372-1375 ◽  
Author(s):  
Shanghua Fan ◽  
Defeng Li ◽  
Joy Fleming ◽  
Yuan Hong ◽  
Tao Chen ◽  
...  
Keyword(s):  
Crystallographic Analysis ◽  
Unit Cell ◽  
Monoclinic Space Group ◽  
X Ray Diffraction ◽  
Unit Cell Parameters ◽  
X Ray ◽  
Cell Parameters ◽  
Essential Enzyme ◽  
Mycobacterial Growth

7-Keto-8-aminopelargonic acid synthase (KAPA synthase; BioF) is an essential enzyme for mycobacterial growth that catalyses the first committed step in the biotin-synthesis pathway. It is a pyridoxal 5′-phosphate (PLP)-dependent enzyme and is a potential drug target. Here, the cloning, expression, purification and crystallization of KAPA synthase fromMycobacterium smegmatis(MsBioF) and the characterization of MsBioF crystals using X-ray diffraction are described. The crystals diffracted to 2.3 Å resolution and belonged to the monoclinic space groupP21, with unit-cell parametersa= 70.88,b= 91.68,c= 109.84 Å, β = 97.8°. According to the molecular weight of MsBioF, the unit-cell parameters and the self-rotation function map, four molecules are present in each asymmetric unit with aVMvalue of 2.06 Å3 Da−1and a solvent content of 40.20%.

Preparation and characterization of a novel solid solution of aluminum in tungsten carbide by mechanically activated high-temperature reaction

2006 ◽  
Vol 21 (7) ◽  
pp. 1700-1703 ◽  
Author(s):  
Junmin Yan ◽  
Xianfeng Ma ◽  
Wei Zhao ◽  
Huaguo Tang ◽  
Changjun Zhu ◽  
...  
Keyword(s):  
Solid Solution ◽  
High Temperature ◽  
Substitutional Solid Solution ◽  
Phase Identification ◽  
Temperature Reaction ◽  
X Ray Diffraction ◽  
X Ray ◽  
Cell Parameters ◽  
High Temperature Reaction

In this work, a novel substitutional solid solution (W0.8Al0.2)C was synthesized by mechanically activated high-temperature reaction. X-ray diffraction was used for phase identification during the whole reaction process. Environment scanning electronic microscopy–field emission gun and energy dispersive x-ray were used to investigate the microstructure and the quantitative material composition of the specimen. (W0.8Al0.2)C was found to crystallize in the WC-type, and the cell parameters were a = 2.907(1) Å and c = 2.837(1) Å. The hardness of (W0.8Al0.2)C was tested to be 19.3 ± 1 GPa, and the density was 13.19 ± 0.05 g cm−3.

Crystallization and preliminary X-ray diffraction analysis of the NAD-dependent non-phosphorylating GAPDH of the hyperthermophilic archaeon Thermoproteus tenax

2000 ◽  
Vol 56 (1) ◽  
pp. 89-91 ◽  
Author(s):  
Nina A. Brunner ◽  
Dietmar A. Lang ◽  
Matthias Wilmanns ◽  
Reinhard Hensel
Keyword(s):  
Diffraction Limit ◽  
Polyethylene Glycols ◽  
Asymmetric Unit ◽  
X Ray Diffraction ◽  
Hanging Drop ◽  
Unit Cell Parameters ◽  
X Ray ◽  
Cell Parameters ◽  
Diffusion Technique ◽  
Thermoproteus Tenax

Recombinant non-phosphorylating NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPN) of the hyperthermophilic crenarchaeote Thermoproteus tenax has been overexpressed, purified and crystallized using the hanging-drop vapour-diffusion technique. Crystals of different habits were obtained from several precipitant solutions (salts and polyethylene glycols). Preliminary X-ray analysis was performed with crystals grown in ammonium formate, which belonged to the primitive hexagonal space group P622, and had unit-cell parameters a = b = 184.8, c = 133.0 Å, γ = 120°. Assuming a molecular weight of 55 kDa, a Matthews parameter of 3.3 Å3 Da−1 is calculated assuming two molecules per asymmetric unit. The diffraction limit of these crystals is 2.5 Å resolution.

scholarly journals SYNTHESIS AND STRUCTURAL CHARACTERIZATION OF THE 1:1 PROTON TRANSFER SALT OF MALONIC ACID WITH P-CHLOROANILINE

2017 ◽  
Vol 14 (28) ◽  
pp. 66-71
Author(s):  
Gerson E. DELGADO ◽  
Lusbely BELANDRIA ◽  
Asiloé J. MORA ◽  
Julia BRUNO-COLMENÁREZ ◽  
Gustavo MARROQUÍN
Keyword(s):  
Hydrogen Bonds ◽  
Malonic Acid ◽  
Crystal Packing ◽  
Chemical Properties ◽  
Supramolecular Assembly ◽  
X Ray Diffraction ◽  
Monoclinic System ◽  
X Ray ◽  
Cell Parameters

The design of multicomponent crystals offers a means to modify the physicochemical properties of crystals without altering the chemical properties of a particular molecule. In this study, a multicomponent crystal, the salt of malonic acid with p-chloroaniline, was synthetized and structurally characterized. The title compound wasprepared by grinding in an agate mortar, and its structure was studied by powder and single-crystal X-ray diffraction. This compound crystallize in the monoclinic system with space group P21/c, Z = 4, and unit cell parameters a = 12.9776(7)Å, b = 9.2308(5)Å, c = 8.5170(5)Å, β = 93.474(3)°. The multicomponent compound, p-chloroanilinium semi-malonate, can be described as an ionic ensemble assisted by hydrogen bonds established between p-chloroanilinium cations and semi-malonate anions. The molecular structure and crystal packing are stabilized mainly by intermolecular O-H•••O and N-H•••O hydrogen bonds interactions. The molecules construct a supramolecular assembly with a two-dimensional hydrogen bonded network along the ca plane.

scholarly journals Phase equilibria in the SnBi2Te4MnBi2Te4 system and characterization of the Sn1-xMnxBi2Te4 solid solutions

2020 ◽  
Vol 21 (1) ◽  
pp. 113-116 ◽  
Author(s):  
E. Orujlu
Keyword(s):  
Phase Equilibria ◽  
Solid Solutions ◽  
Continuous Series ◽  
X Ray Diffraction ◽  
Incongruent Melting ◽  
Solidus Curve ◽  
Entire Concentration Range ◽  
X Ray ◽  
Cell Parameters

The phase diagram of the SnBi2Te4-MnBi2Te4 system was established over the entire concentration range by means of differential thermal analysis and powder X-ray diffraction techniques. It was shown that the system is non-quasi-binary due to the incongruent melting character of SnBi2Te4 and MnBi2Te4 compounds, but it is stable below solidus. The formation of a continuous series of solid solutions with the tetradymite-like layered structure was observed. Due to ionic radius differences of Mn2+ and Sn2+, both unit cell parameters of solid solutions increase linearly with the increasing amount of Sn. Phase equilibria above the solidus curve cannot be completed until the SnTe-MnTe-Bi2Te3 system fully studied.

Hydrothermal synthesis and characterization of the praseodymium borate-nitrate Pr[B5O8(OH)(H2O)0.87]NO3·2H2O

2017 ◽  
Vol 72 (9) ◽  
pp. 677-685
Author(s):  
Teresa S. Ortner ◽  
Hubert Huppertz
Keyword(s):  
Hydrothermal Synthesis ◽  
Synthesis And Characterization ◽  
The Novel ◽  
X Ray Diffraction ◽  
Unit Cell Parameters ◽  
X Ray ◽  
Cell Parameters ◽  
Rare Earth Borate ◽  
Ir And Raman

AbstractThe praseodymium borate-nitrate Pr[B5O8(OH)(H2O)0.87]NO3·2H2O was obtained in a hydrothermal synthesis. It crystallizes monoclinically in the space groupP21/n(no. 14) with four formula units (Z=4) and unit cell parameters ofa=641.9(3),b=1551.8(7),c=1068.4(5) pm, withβ=90.54(2)° yieldingV=1.0643(8) nm3. The defect variant constitutes the missing member in the series of isostructural, early rare earth borate-nitrates of the compositionRE[B5O8(OH)(H2O)x]NO3·2H2O [RE=La (x=0; 1), Ce (x=1), Nd (x=0.85), Sm (x=0)]. In addition to powder and single-crystal X-ray diffraction data, the novel borate-nitrate was characterized through IR and Raman spectroscopy.

Synthesis and characterization of a new trimetallic compound (NH4)Ni2.4Co0.6O(OH)(MoO4)2•1.5H2O

2014 ◽  
Vol 29 (4) ◽  
pp. 379-382 ◽  
Author(s):  
Sandra Amaya ◽  
Johana Arboleda ◽  
Adriana Echavarría
Keyword(s):  
Thermal Analysis ◽  
Laser Raman Spectroscopy ◽  
Synthesis And Characterization ◽  
X Ray Diffraction ◽  
Unit Cell Parameters ◽  
X Ray ◽  
Cell Parameters ◽  
Laser Raman ◽  
Fourier Transformed Infrared Spectroscopy

A new trimetallic compound with formula (NH4)Ni2.4Co0.6O(OH)(MoO4)2•1.5H2O was obtained by hydrothermal synthesis. The solid was characterized by X-ray diffraction, thermal analysis (thermogravimetric analysis and differential thermal analysis), Fourier-transformed infrared spectroscopy, Laser Raman spectroscopy, and chemical analysis by atomic absorption, confirming the formation of the layered phase ϕy. Crystallographic studies showed that the compound obtained is trigonal with hexagonal unit-cell parameters, a = 6.0468 ± 0.0016 Å and c = 21.8433 ± 0.0001 Å, and space group R-3m.

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