Study on comprehensive line selection and continuous line selection for neutral point noneffectively grounded system

2004 ◽  
Author(s):  
Qi Zheng ◽  
Chen Jiongcong ◽  
Yang Yihan
Keyword(s):  
Neutral Point ◽  
Continuous Line ◽  
Line Selection ◽  
Selection For

Doxorubicin selection for MDR1/P-glycoprotein reduces swelling-activated K+ and Cl- currents in MES-SA cells

1996 ◽  
Vol 270 (4) ◽  
pp. C1029-C1036 ◽  
Author(s):  
D. B. Luckie ◽  
M. E. Krouse ◽  
T. C. Law ◽  
B. I. Sikic ◽  
J. J. Wine
Keyword(s):  
Cell Line ◽  
Anion Channel ◽  
Uterine Sarcoma ◽  
Protein Levels ◽  
P Glycoprotein ◽  
Whole Cell Patch Clamp ◽  
Cl Channels ◽  
Line Selection ◽  
Selection For ◽  
K Currents

To test the hypothesis that P-glycoprotein enhances swelling currents through regulation of volume-sensitive Cl- channels [recently termed VSOAC (volume-sensitive osmolyte and anion channel)], a human uterine sarcoma cell line (MES-SA) and its doxorubicin-selected counterpart (Dx5) were studied. P-glycoprotein mRNA and protein levels were detected only in Dx5 cells. However, whole cell patch-clamp experiments showed that swollen Dx5 cells (n = 5) produced smaller VSOAC currents than MES-SA cells (n = 4; 106 +/- 26 pA/pF vs. 232 +/- 76 pA/pF at 90 mV). In radioisotopic efflux experiments, both swelling-activated 125I (Cl-) currents (n = 15) and 86Rb (K+) currents (n = 8) were found to be two-to fourfold smaller in the Dx5 (high P-glycoprotein) cells. Inhibitors of P-glycoprotein showed no specificity for the doxorubicin-selected cells (Dx5). Dideoxyforskolin (100 microM) blocked swelling-activated 125I efflux equally in both cell lines, whereas 100 microM verapamil had no effect. Thus, in this cell line, selection for P-glycoprotein expression is associated with reduced swelling currents. These findings suggest that P-glycoprotein expression does not directly facilitate VSOAC.

scholarly journals Breeding strategies and advances in line selection for Fusarium head blight resistance in wheat

2017 ◽  
Vol 42 (3) ◽  
pp. 165-174 ◽  
Author(s):  
Barbara Steiner ◽  
Maria Buerstmayr ◽  
Sebastian Michel ◽  
Wolfgang Schweiger ◽  
Marc Lemmens ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Fusarium Head Blight Resistance ◽  
Blight Resistance ◽  
Breeding Strategies ◽  
Head Blight ◽  
Line Selection ◽  
Selection For

scholarly journals Response to selection for increased pupa weight in Tribolium castaneum as related to population structure

1977 ◽  
Vol 30 (3) ◽  
pp. 237-246 ◽  
Author(s):  
Alan J. Katz ◽  
Franklin D. Enfield
Keyword(s):  
Random Mating ◽  
Selection Response ◽  
Response To Selection ◽  
Short Term ◽  
Selection Systems ◽  
Line Selection ◽  
Selection For ◽  
Seven Generations ◽  
Generation Period ◽  
Term Response

SUMMARYThe effectiveness of selection for increased pupa weight in Tribolium was compared for three different selection systems. In all three systems the same number of breeding individuals was used each generation. Population L was a large random mating population with 24 males and 48 females selected each generation. The C4 and C8 populations were each divided into 6 subpopulations (lines) consisting of 4 males and 8 fem ales. Each of the three populations was replicated. In C4, selection for pupa weight was within lines for three generations, followed by a generation of among-line selection when the best two out of six lines were selected. These lines were then crossed to produce 6 new subpopulations, and the cycle was repeated. The C8 population was handled in exactly the same manner except that seven generations of selection within lines were practised before each generation of among-line selection. Selection response for the 42-generation period was significantly greater in the L population than in either subdivided population. No consistent differences among the selection systems were apparent when evaluating short-term response for the first 12 generations of the experiment. The results were interpreted as indicating that the influence of multiple-peak epistasis was not of major importance for this trait in determining ultimate response to selection when starting from a base population of previously unselected lines and utilizing a within- and among-line selection regime.

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