With the rapid development of urbanization in China, the ecological environment is more and more seriously threatened; most cities should be focused on urban ecological environment construction and protection. In this paper, we choose the vegetation, slope, elevation, surface river system as the factors, and their weights were determined by analytic hierarchy process (AHP). Applying with GIS spatial analysis technique, the ecological sensitivity of the four factors was divided into four grades which contains high sensitive area, moderately sensitive area, low sensitive area and insensitive area areas occupied 9.43%, 36.46%, 50.61% and 3.50%. There existed a significant spatial differentiation in the ecological sensitivity of Enshi city. This study could provide scientific references for the city's environmental protection and economic development, to help its economic growth.