In this paper, we extend the quantum game theory of Prisoner’s Dilemma to the N-player case. The final state of quantum game theory of N-player Prisoner’s Dilemma is derived, which can be used to investigate the payoff of each player. As demonstration, two cases (2-player and 3-player) are studied to illustrate the superiority of quantum strategy in the game theory. Specifically, the non-unique entanglement parameter is found to maximize the total payoff, which oscillates periodically. Finally, the optimal strategic set is proved to depend on the selection of initial states.