Technique for the detection of human pathogen via maintenance of leishmania promastigote in blood agar culture medium

2018 ◽  
Author(s):  
Sana Ullah ◽  
Irfan Ali ◽  
Rabya Fatima ◽  
Fazal Haq
Keyword(s):  
Culture Medium ◽  
Blood Agar ◽  
Human Pathogen ◽  
Agar Culture

Chemical control of fungal growth and ochratoxin A production by Aspergillus isolated from Moroccan grapes

2018 ◽  
Vol 7 (2) ◽  
pp. 84-91
Author(s):  
Adil Laaziz ◽  
Souad Qjidaa ◽  
Yousra El Hammoudi ◽  
Abdelouahed Hajjaji ◽  
Amina Bouseta
Keyword(s):  
Ochratoxin A ◽  
Radial Growth ◽  
Culture Medium ◽  
Fungal Growth ◽  
Sublethal Concentration ◽  
Agar Culture ◽  
Total Inhibition ◽  
Yeast Autolysate ◽  
Germination And Growth ◽  
Czapek Yeast Autolysate Agar

The aim of this study was to evaluate the effect of three fungicides azoxystrobin (Ortiva), hexaconazole (Hexa) and pyrimethanil (Pyrus) for their ability to inhibit the radial growth and ochratoxin A (OTA) production by five ochratoxigenic strains of Aspergillus carbonarius and A. niger previously iso-lated from Moroccan grapes. Our results showed that, the addition of the fungicides to the Czapek Yeast Autolysate agar culture medium reduced the growth of the ochratoxigenic strains. Pyrimethanil caused total inhibition of spore germination and growth of the five strains, for all dose tested. Where-as hexaconazole totally inhibited the growth of 4 strains and gave growth for the MUCL 49227 strain (2.67 mm/day) at sublethal concentration. The reduction in radial growth was less marked for azoxystrobin, with growth rate varying between 0 and 6.37 mm/day depending on the strain and the azoxystrobin concentration. Analysis of variance showed that the effect of single factors (fungicides, concentration and strain) and their interactions on growth and OTA production were highly significant (P=0.000).These findings suggest that the use of tested fungicides have to potential for reduction in production of OTA.

scholarly journals Efficiency of Blotter test and agar culture medium to detect Fusarium graminearum and Pyricularia grisea in wheat seeds

2017 ◽  
Vol 39 (3) ◽  
pp. 297-302
Author(s):  
Meyriele Pires de Camargo ◽  
Maria Heloisa Duarte de Moraes ◽  
José Otávio Machado Menten
Keyword(s):  
Fusarium Graminearum ◽  
Culture Medium ◽  
Potato Dextrose Agar ◽  
Pyricularia Grisea ◽  
Agar Culture ◽  
Hypochlorite Solution ◽  
Three Samples ◽  
Seed Health ◽  
Incubation Temperatures ◽  
Wheat Seeds

Abstract: Seeds can be considered one of the most efficient forms to disseminate pathogens. Therefore, the use of healthy seeds is extremely important to establish a crop, and seed health testing must be performed to determine the seed sanitary quality. This study aimed to compare the efficiency of seed health testing to detect Pyricularia grisea and Fusarium graminearum in three samples of wheat seeds. Methods evaluated were Blotter test with freezing (BTF) and potato-dextrose-agar culture medium (PDA). The incubation temperatures were 20 ºC and 25 ºC. From each sample, a subsample was submitted to seed asepsis using hypochlorite solution (1%) and another analyzed without seed asepsis. The temperatures evaluated did not influence the detection of the pathogens. P. grisea incidence ranged from 4.5 to 17% with BTF without seed asepsis. The BTF with seed asepsis and PDA (with and without seed asepsis) presented pathogen incidence no higher than 1.5%. Moreover, results suggested that most part of P. grisea inoculum was presented externally on seed tissues. PDA medium was more efficient to detect F. graminearum, independently of seed asepsis. By this method, pathogen incidence ranged from 3 to 39%. F. graminearum incidence using BTF with seed asepsis varied from 0.5 to 1.5% and BTF without seed asepsis presented a pathogen incidence of 5.0 to 12.5%. The Blotter test with freezing was more efficient to detect P. grisea while the PDA medium was more efficient to detect F. graminearum.

scholarly journals Obtaining monokaryotic and dikaryotic mycelial cultures of two Amazonian strains of Geastrum (Geastraceae, Basidiomycota)

2020 ◽  
Vol 50 (1) ◽  
pp. 61-67
Author(s):  
Marcos Diones Ferreira SANTANA ◽  
Ruby VARGAS-ISLA ◽  
Janaina da Costa NOGUEIRA ◽  
Thiago ACCIOLY ◽  
Bianca Denise Barbosa da SILVA ◽  
...  
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Brazilian Amazon ◽  
Potato Dextrose Agar ◽  
Morphological Description ◽  
Agar Culture ◽  
Biotechnological Potential ◽  
Sexual Crosses ◽  
High Diversity

ABSTRACT The high diversity of the genus Geastrum and the difficulty of obtaining mycelial cultures impairs the study of the ecophysiology and the exploration of the biotechnological potential of the taxon. In this study, different culture media were tested to obtain mycelial cultures for G. lloydianum and G. subiculosum collected in the Brazilian Amazon. Data on spore germination, and isolation of monokaryotic cultures and in vitro sexual reproduction are presented, as well as a brief morphological description of the cultures obtained. For both species, Potato Dextrose Agar (PDA) was the most promising of the tested culture media. The highest growth in agar culture ever recorded for this genus is reported (4.9 mm per week for G. lloydianum and 7.5 mm for G. subiculosum). In the PDA culture medium, spores germinated after 35-40 days of incubation and the isolation of monokaryotic cultures of the two species, as well as in vitro sexual crosses, were successfully performed.

scholarly journals In Vitro Effect ofZingiber officinaleExtract on Growth ofStreptococcus mutansandStreptococcus sanguinis

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Arash Azizi ◽  
Shabnam Aghayan ◽  
Saeed Zaker ◽  
Mahdieh Shakeri ◽  
Navid Entezari ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Culture Medium ◽  
Zingiber Officinale ◽  
Bacterial Suspension ◽  
Tooth Decay ◽  
Agar Culture ◽  
Solid Agar ◽  
Vitro Effect ◽  
Serial Dilutions

Background and Objectives. Tooth decay is an infectious disease of microbial origin. Considering the increasing prevalence of antibiotic resistance due to their overuse and also their side effects, medicinal plants are now considered for use against bacterial infections. This study aimed to assess the effects of different concentrations ofZingiber officinaleextract on proliferation ofStreptococcus mutansandStreptococcus sanguinisin vitro.Materials and Methods. In this experimental study, serial dilutions of the extract were prepared in two sets of 10 test tubes for each bacterium (total of 20). Standard amounts of bacterial suspension were added; 100ƛof each tube was cultured on prepared solid agar plates and incubated at 37°C for 24 hours. Serial dilutions of the extract were prepared in another 20 tubes and 100ƛof each tube was added to blood agar culture medium while being prepared. The mixture was transferred to the plates. The bacteria were inoculated on plates and incubated as described.Results. The minimum inhibitory concentration (MIC) was 0.02 mg/mL forS. mutansand 0.3 mg/mL forS. sanguinis. The minimum bactericidal concentration (MBC) was 0.04 mg forS. mutansand 0.6 mg forS. sanguinis.Conclusion.Zingiber officinaleextract has significant antibacterial activity againstS. mutansandS. sanguiniscariogenic microorganisms.

scholarly journals A note on some physical conditions for cultivating Oxyrrhis marina

1959 ◽  
Vol 38 (3) ◽  
pp. 599-604 ◽  
Author(s):  
M. R. Droop
Keyword(s):  
Culture Medium ◽  
Sea Water ◽  
Soil Extract ◽  
Agar Culture ◽  
Wire Loop ◽  
Oxyrrhis Marina ◽  
Physical Conditions ◽  
Series Of Experiments ◽  
Saccharomyces Exiguus

This note concerns a series of experiments to determine the best conditions of salinity, temperature and pH for cultivating the euryhaline phagotrophic dinoflagellate Oxyrrhis marina Dujardin.The strain of Oxyrrhis employed was isolated from a brackish pool at Tvarminne, Finland (Droop, 1953a, b). The culture medium for the experiments contained soil extract and an artificial sea water, SW 1 (NaCl, MgCl26H2O, KCl, and CaSO42H2O in the proportions by weight 15:2.5:0.4:0.5), and for food a small quantity of the yeast Saccharomyces exiguus was administered daily from an agar culture with a wire loop.

scholarly journals Effect of Fungicides on Colony Growth of Colletotrichum Lindemuthianum (Sacc. & Magn.) Scrib.

2008 ◽  
Vol 48 (2) ◽  
pp. 201-212
Author(s):  
Juliana Sartori ◽  
Antonio Maringoni
Keyword(s):  
Culture Medium ◽  
High Sensitivity ◽  
Colony Growth ◽  
Potato Dextrose Agar ◽  
Colletotrichum Lindemuthianum ◽  
Cross Resistance ◽  
Agar Culture ◽  
Thiophanate Methyl ◽  
Low Sensitivity

Effect of Fungicides on Colony Growth ofColletotrichum Lindemuthianum(Sacc. & Magn.) Scrib.Colletotrichum lindemuthianum(Sacc. & Magn.) Scrib. is the causal agent of the anthracnose of common bean (Phaseolus vulgarisL.), a fungal disease of a great significance in brazilian bean cultures. The goals of this work were to evaluate thein vitrocolony growth and to determine the ED50interval of twentyC. lindemuthianumisolates from different regions of Brazil to five fungicides of different active ingredients and to some blendings (carbendazim, chlorothalonil, thiophanate-methyl, chlorothalonil + thiophanate-methyl, trifloxystrobin, propiconazole and trifloxystrobin + propiconazole), at concentrations of 0, 1, 10, 100 and 1000 μg/ml, in a potato-dextrose-agar culture medium. The results revealed seven isolates with low sensitivity to carbendazim and thiophanate-methyl (ED50interval greater than 1000 μg/ml) thus suggesting cross-resistance. Isolate sensitivity to chlorothalonil ranged from ED50interval less than 1 μg/ml to greater than 1000 μg/ml. Those isolates with high sensitivity to thiophanate-methyl, ED50interval less than 1 μg/ml, did also show it with respect to chlorothalonil + thiophanate-methyl. Sixteen isolates showed a high sensitivity to trifloxystrobin with a ED50interval less than 1 μg/ml. Nineteen isolates ofC. lindemuthianumshowed high sensitivity to propiconazole and to trifloxystrobin + propiconazole with ED50interval less than 1 μg/ml. Isolates with low sensitivity to carbendazim and thiophanate-methyl were sensitive to propiconazole and to trifloxystrobin + propiconazole. Variability was found in the sensitivity of the colony growth ofC. lindemuthianumisolates from different regions of Brazil to the fungicides evaluated.

Agar Culture Medium modified to approximate Soil Conditions

Nature ◽  
1970 ◽  
Vol 227 (5263) ◽  
pp. 1161-1161 ◽  
Author(s):  
J. PROSKAUER ◽  
R. BERMAN
Keyword(s):  
Culture Medium ◽  
Soil Conditions ◽  
Agar Culture

scholarly journals Hongos simbiontes de hormigas cortadoras de hojas del género Acromyrmex en bosques secos del centro de Argentina

2021 ◽  
Vol 9 (1) ◽  
pp. 162-172
Author(s):  
David Pelissero ◽  
Francisco Kuhar ◽  
Mariana Pereyra ◽  
Eduardo Nouhra
Keyword(s):  
Culture Medium ◽  
Taxonomic Diversity ◽  
Ribosomal Gene ◽  
Ecological Processes ◽  
Agar Culture ◽  
Chaco Serrano ◽  
Clade B ◽  
Symbiotic Fungi ◽  
Leucocoprinus Gongylophorus ◽  
Well Differentiated

Fungi associated with leaf-cutter ants (LCAs) play a fundamental role in Neotropical ecosystems. The association with fungi allows LCAs to be the dominant herbivores and in this way influence ecological processes. In order to know the taxonomic diversity of fungal symbionts associated with LCAs, ten sampling sites were selected corresponding to the Chaqueño Serrano district and the Algarrobo district. Samples of fungi grown by leaf cutter ants were collected, a part of the fungal material was grown in potato agar culture medium with dextrose and another was stored in a CTAB buffer. Subsequently, amplifications of the ITS1, ITS2 and 5.8S ribosomal gene were carried out. A phylogenetic analysis (Maximum Likelihood and Bayesian) was performed with the sequences. Results show that the leaf cutter ants in the sampled region cultivate symbiotic fungi of two well-differentiated phylogenetic clades (known as A and B). The agar cultures showed a slow growth, with development of globose structures named gongylidia that are grouped together forming staphyla (clusters). Among the ants studied, Acromyrmex striatus is associated with fungi from both clades. In the Espinal forest (Algarrobo district) A. striatus was found associated with clade B fungi (Leucocoprinus sp.) and in the Chaco Serrano (Chaqueño Serrano district) with clade A fungi (Leucocoprinus gongylophorus). While Acromyrmex crassispinus and A. heyeri included in the study exclusively cultivate L. gongylophorus from clade A fungi regardless of the region they inhabit. In addition, the first molecular identification of the fungus L. gongylophorus cultivated by Acromyrmex silvestrii is reported.

Sign in / Sign up

Export Citation Format

Share Document