Mandarin fruits are one of the most popular among the Citrus genus. They are
consumed because of their nutritional and health benefits, as well as
pleasant taste and smell. This paper describes the identification and
characterization of Penicillium expansum, isolated from molded mandarin
fruits. The obtained isolates were cultivated on five media [Czapek Yeast
Autolysate agar (CYA), MEA (Malt extract agar), Creatine sucrose agar
(CREA), Yeast extract sucrose agar (YES), and Oatmeal agar (OA)] and at five
different incubation temperatures (5, 15, 25, 30, and 37 ?C). Isolates were
sequenced for two molecular loci: internal transcribed spacer and
beta-tubulin. Based on the results from morphological, physiological,
molecular, and phylogenetic analyses, the recovered isolates were identified
as P. expansum. The isolated species was confirmed as pathogenic to mandarin
fruits in a pathogenicity test. To the best of our knowledge, this is the
first report of P. expansum as a postharvest pathogen of mandarin fruit in
Serbia.