Influence of biotechnological parameters on the yield of macrostructures from unfertilized seed germs of diploid sugar beet

Purpose. To determine the influence of biotechnological parameters on the yield of macrostructures from unfertilized seed germs of diploid sugar beet. Methods. Biotechnological, laboratory, analytical, statistical. Results. It was found that the use of 35% sodium hypochlorite solution at an exposure of 40 min allows to obtain from 73.13 to 75.83% of sterile seed germs. Exposure of 50 min allows to obtain the sterility of the source material from 83.58 to 85.39%. Sterilization of explants for 60 min allows to obtain sterility of the source material from 86.88 to 92.80%. The share of infected seed germs with increasing exposure decreased from 20.09–22.14 to 6.52–12.61%. The yield of macrostructures has been experimentally confirmed to significantly depend on breeding genotype and type of medium. The largest number of calluses (10–80%) was formed with the use of the Hamburg and Eveleg’s medium. With the use of he Murasige and Skoog’s medium, their share was 10–35%. Noteworthy, in breeding genotypes 07–181, 80% of genotypes formed buds and 35% formed calluses in the Hamburg and Eveleg’s medium. Of breeding genotypes 07–178, 55% of genotypes formed a callus and 80% buds. Conclusions. As a result of the conducted researches the influence of biotechnological parameters (exposure to 35% solution of sodium hypochlorite, type of nutrient medium) on the yield of macrostructures from unfertilized seed germs of diploid sugar beet was determined. It is optimal to carry out treatment with 35% sodium hypochlorite solution for 50–60 minutes, regardless of the selection number of sugar beet. To obtain macrostructures from unfertilized seed germs of diploid sugar beet, it is necessary to use the Hamburg and Eveleg’s medium for breeding genotypes 07–188, 07–178 and 07–181.

Estudo comparativo de tratamento da estomatite protética pelo reembasamento ou substituição das próteses totais

This clinic survey aimed at comparing two different treatments for patients using full upper denture and having clinical Denture Stomatitis diagnosis. Thirty patients were selected and divided into two groups. Group I included 18 patients, whose dentures were replaced by new ones. Group II included 12 patients, whose full dentures currently used were relined. Patients were followed-up during 8 weeks. Patients of both groups were required to remove their dentures at night to be disinfected with Sodium Hypochlorite solution. The results observed show Denture Stomatitis was cured within 4 weeks in 90% the cases for both groups, showing no significant statistical differences. It may be concluded that both dentures replacement and their filling, associated to hygiene and disinfection show efficacy in Denture Stomatitis control.

Studies on the Separation and Purification of the Caulis sinomenii Extract Solution Using Microfiltration and Ultrafiltration

The separation and purification process of alkaloids faces great challenges of pollution, high energy consumption and low continuity. In this study, the effects of ceramic microfiltration (MF) membrane (membrane pore size of 0.50 μm, 0.20 μm, 0.05 μm) and organic ultrafiltration (UF) membrane (membrane molecular weight cut-off of 10 KDa and 1 KDa) on the separation and purification of Caulis sinomenii extract solution in pilot scale were studied. The cleaning effects of different cleaning methods (pure water, 1% HCl-NaOH, 1% sodium hypochlorite) were investigated. The experimental results indicated that 0.05 μm ceramic membrane and 1 kDa UF membrane have higher sinomenine hydrochloride (SH) permeabilities and total solids (TS) removal rates. The ceramic membrane was circulating cleaned by 1% sodium hypochlorite solution for 1 h; the membrane flux can be restored to more than 90% of the original, the membrane flux of 1 kDa UF membrane can be restored to 99.2% of the original by pure water washing. From the above study, the optimal technic parameters was determined in which 0.05 μm ceramic MF membrane and 1 kDa UF membrane were used to separate and purify the Caulis sinomenii extract solution to remove the invalid ingredients, and the two kinds of membranes were cleaned with 1% sodium hypochlorite solution and pure water, respectively, to keep satisfactory membrane fluxes. The study provided an environment-friendly alternative for the separation and purification of alkaloids in natural products, which has a good prospect for the industrial application.

Cutaneous wound healing modulation of lesions infected by Staphylococcus epidermidis and treated with 4% stabilized sodium hypochlorite solution

Background: Stabilized sodium hypochlorite solution at 4% (4% NaClO) is a product with healing properties but still little studied scientifically. Here we evaluated the administration of 4% NaClO in a model of clean and infected skin lesions. Methods: Lesions were obtained on the dorsal region of Wistar rats. The wounds of some animals were inoculated with Staphylococcus epidermidis (50uL, 10-7) and clean and infected lesions were treated with Trofodermin® or 4% NaClO (diluted at 0.4%) as the following groups: Trofodermin®; S. epidermidis + Trofodermin®; 4% NaClO; S. epidermidis + 4% NaClO. Wounds sizes were daily measured and the excision of lesions was performed on day 8 for histopathological evaluation and immunohistochemical studies of Annexin A1 (AnxA1), receptors for formylated peptides (FPR)1 and FPR2 and vascular endothelial growth factor (VEGF). Levels of interleukin (IL)-1β and tumor necrosis factor (TNF)-α in the supernatants of the macerated lesions were also measured. Results: Wounds treated with 4% NaClO closed faster than those treated with Trofodermin®, especially in the infected group. 4% NaClO reduced the expressions of AnxA1, FPR1, FPR2 and VEGF, as well as decreased the levels of IL-1β. Conclusion: These results pointed the potential of 4% NaClO in wound healing which opens the possibility for new therapeutic strategies.

Comparison of the Clinical Efficacy of 2.5% Non-Heated Sodium Hypochlorite and 2.5% Heated Sodium Hypochlorite as Irrigation to Control Postoperative Pain in Single Visit Root Canal Treatment

Objective: To compare the clinical efficacy of 2.5% non-heated sodium hypochlorite and 2.5% heated sodium hypochlorite as irrigation to control postoperative pain in single visit root canal treatment. Study Design: Comparative analytical study Place and Duration: Operative dentistry department of Liaquat University of Medical and Health Sciences, Jamshoro for 6 months duration from March 2017 to September 2017. Material and Methods: A total of 60 patients with single rooted maxillary and mandibular permanent teeth were randomly allocated into two groups. 30 patients were in group A and treated with non heated sodium hypochlorite solution and 30 patients in group B were treated with 2.5% heated sodium hypochlorite solution. In both groups, the level of pain was measured postoperatively after 48 hours by using Heft Parker visual Analog Scale of 10 and information was collected on Performa. Results: The average age of the patients was 28.62±6.06 years. There were 40(66.7%) male and 20(33.3%) female. Pain was reduced in both groups but there were no significant difference in reduction of pain between groups (p=0.640). Conclusion: Severity of pain was not statistically significant between groups. At 48 hours, Pain was reduced in both groups but there were no significant difference in reduction of pain between groups. Heated sodium hypochlorite to relieve postoperative pain, it should be new trend for recommendation for treatment of irreversible pulpitis in single visit root canal treatment. Key Words: Root canal, Heated sodium hypochlorite, Postoperative pain.

Evaluation of the influence of UV radiation and sodium hypochlorite on the shelf life of strawberries

It is common to combine methods to increase the shelf life of a raw material or product. In this study, we sought to understand the relationship between ultraviolet (UV) radiation and sodium hypochlorite (NaOCl) in attenuating the proliferation of fungus and its deteriorating effect on strawberries. The UV light intensities of 125, 250 and 400 Watts (W) and sodium hypochlorite were tested in concentrations 0.5, 1.0 and 1.5 ppm. The strawberries were sprayed with sodium hypochlorite solutions and then kept for 1 minute in UV light chambers, to then be stored in transparent sealed plastic containers. The strawberry samples with the longest life were those that were sprayed with 0.5 and 1.0 ppm sodium hypochlorite solution combined with exposure to 125 W UV light. These samples had a shelf-life extension of about five days compared to strawberries without any kind of treatment. Thus, it is possible to state that this combination is efficient in cleaning the fruit without causing damage.

Estudo comparativo de tratamento da estomatite protética pelo reembasamento ou substituição das próteses totais

This clinic survey aimed at comparing two different treatments for patients using full upper denture and having clinical Denture Stomatitis diagnosis. Thirty patients were selected and divided into two groups. Group I included 18 patients, whose dentures were replaced by new ones. Group II included 12 patients, whose full dentures currently used were relined. Patients were followed-up during 8 weeks. Patients of both groups were required to remove their dentures at night to be disinfected with Sodium Hypochlorite solution. The results observed show Denture Stomatitis was cured within 4 weeks in 90% the cases for both groups, showing no significant statistical differences. It may be concluded that both dentures replacement and their filling, associated to hygiene and disinfection show efficacy in Denture Stomatitis control.

Electrochemical detoxification in cancer patients after multiorgan surgery with severe endogenous intoxication

Background. Enteral insufficiency syndrome accompanies the development of many acute diseases of the abdominal cavity. According to the statistics, advanced and multi-organ surgical intervention in oncosurgery within the period from 2019 to 2020 resulted in enteral insufficiency being a complication in 39% of all cases, regardless of anatomical and physiological area, while complications in the form of endogenous intoxication syndrome made up 68% of cases. Purpose – studying the effectiveness of treatment of enteral insufficiency syndrome in cancer patients after multiorgan surgery with severe endogenous intoxication by means of indirect electrochemical detoxification with sodium hypochlorite solution. Materials and methods. The study involved 71 cancer patients who underwent multi-organ surgery on different anatomical and physiological areas. The patients were divided into 2 groups: treatment group (n=36) provided with indirect electrochemical detoxification by means of sodium hypochlorite solution at a concentration of 0.06%, comparison group (n=35) undergoing treatment according to standard schemes. The groups were comparable in age and anatomical and physiological areas that were operated on (surgery was performed on the chest and mediastinum as well as abdominal organs). Results. The method of electrochemical detoxification with sodium hypochlorite reducing the indicators that reflect blood toxicity and intoxication level was used for treating patients with enteral insufficiency. This was evidenced by decreased concentration of bilirubin by 23.1%, urea by 91.6%, creatinine by 99.4%, LII (leukocytal intoxication index) by 47.2% and procalcitonin by 68.2%. Being applied this method has made it possible to achieve a detoxifying effect early on day one. Conclusions. The findings of complete physical examination of cancer patients after multiorgan surgery with severe endogenous intoxication have shown a practical significance of sodium hypochlorite being included in comprehensive post-surgery treatment in enteral insufficiency syndrome cases. Infusions of 0.06% NaClO solution within 24 hours have been proved to provide a detoxifying effect: they significantly reduce elevated concentrations of bilirubin, creatinine, urea, LII and improve blood rheology. Administering sodium hypochlorite in the suppre-ssion of antioxidant defense mechanisms leads to the activation of oxidative processes. Including sodium hypochlorite in comprehensive post-surgery treatment in enteric insufficiency syndrome has shown a high efficiency.