scholarly journals In Vitro Effect ofZingiber officinaleExtract on Growth ofStreptococcus mutansandStreptococcus sanguinis

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Arash Azizi ◽  
Shabnam Aghayan ◽  
Saeed Zaker ◽  
Mahdieh Shakeri ◽  
Navid Entezari ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Culture Medium ◽  
Zingiber Officinale ◽  
Bacterial Suspension ◽  
Tooth Decay ◽  
Agar Culture ◽  
Solid Agar ◽  
Vitro Effect ◽  
Serial Dilutions

Background and Objectives. Tooth decay is an infectious disease of microbial origin. Considering the increasing prevalence of antibiotic resistance due to their overuse and also their side effects, medicinal plants are now considered for use against bacterial infections. This study aimed to assess the effects of different concentrations ofZingiber officinaleextract on proliferation ofStreptococcus mutansandStreptococcus sanguinisin vitro.Materials and Methods. In this experimental study, serial dilutions of the extract were prepared in two sets of 10 test tubes for each bacterium (total of 20). Standard amounts of bacterial suspension were added; 100ƛof each tube was cultured on prepared solid agar plates and incubated at 37°C for 24 hours. Serial dilutions of the extract were prepared in another 20 tubes and 100ƛof each tube was added to blood agar culture medium while being prepared. The mixture was transferred to the plates. The bacteria were inoculated on plates and incubated as described.Results. The minimum inhibitory concentration (MIC) was 0.02 mg/mL forS. mutansand 0.3 mg/mL forS. sanguinis. The minimum bactericidal concentration (MBC) was 0.04 mg forS. mutansand 0.6 mg forS. sanguinis.Conclusion.Zingiber officinaleextract has significant antibacterial activity againstS. mutansandS. sanguiniscariogenic microorganisms.

Phosphite-based Products in the In vitro Colletotrichum musae Control

2019 ◽  
pp. 1-9
Author(s):  
Maria Luísa Mendes Rodrigues ◽  
Edson Hiydu Mizobutsi ◽  
Paola Junayra Lima Prates ◽  
Paula Virgínia Leite Duarte ◽  
Regina Cássia Ferreira Ribeiro ◽  
...  
Keyword(s):  
Mycelial Growth ◽  
Culture Medium ◽  
Culture Media ◽  
Petri Dish ◽  
State University ◽  
Randomized Design ◽  
Colletotrichum Musae ◽  
Study Laboratory ◽  
Vitro Effect

Aims: The aim of this study was to evaluate the in vitro effect of different phosphite formulations and concentrations on the development of Colletotrichum musae. Sample: to evaluate the inhibition of germination, mycelial growth and sporulation of Colletotrichum musae. Study Design:  Treatments were conducted in a completely randomized design, with 4 replicates, each replicate consisting of 1 Petri dish. Place and Duration of Study:  Laboratory of Post-Harvest Pathology, State University of Montes Claros, between March and October 2017. Methodology: Three different phosphite formulations were used: FCu1 (4% Cu + 20% P2O5), FCu2 (4% Cu + 22% P2O5) at concentrations of 0.5;1.0; 1.5 and 2.0 mL L-1 and FK (42% P2O5 + 27.7% K2O) at concentrations of 0.5; 1.0; 1.5 and 2.0 mg.L-1. Products were incorporated into the respective culture media. Culture medium alone and culture medium + imazalil were used as controls. Petri dishes were housed in BOD chamber at 25°C under a 12 hours photoperiod. Results: Results were submitted to analysis of variance and regression, and means were compared by the Tukey test (P <0.05). Control was compared to the other treatments by the Dunnet's test (P <0.05). Among the tested phosphite formulations, copper and potassium phosphites were found to reduce the mycelial growth of Colletotrichum musae. FCu2 presents a fungicide-like effect from the concentration of 0.5 m.L-1 in the control of conidia production. As for the FCu1, a fungicide-like effect was observed in the control of germination from the concentration of 1.5 mL.L-1. Conclusion: A significant fungistatic effect was observed between the concentrations of the products in the mycelial growth, sporulation and germination obtaining control of up to 100% of the development of C. musae. Copper phosphites were as effective as fungicide in inhibiting fungal development.

scholarly journals Obtaining monokaryotic and dikaryotic mycelial cultures of two Amazonian strains of Geastrum (Geastraceae, Basidiomycota)

2020 ◽  
Vol 50 (1) ◽  
pp. 61-67
Author(s):  
Marcos Diones Ferreira SANTANA ◽  
Ruby VARGAS-ISLA ◽  
Janaina da Costa NOGUEIRA ◽  
Thiago ACCIOLY ◽  
Bianca Denise Barbosa da SILVA ◽  
...  
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Brazilian Amazon ◽  
Potato Dextrose Agar ◽  
Morphological Description ◽  
Agar Culture ◽  
Biotechnological Potential ◽  
Sexual Crosses ◽  
High Diversity

ABSTRACT The high diversity of the genus Geastrum and the difficulty of obtaining mycelial cultures impairs the study of the ecophysiology and the exploration of the biotechnological potential of the taxon. In this study, different culture media were tested to obtain mycelial cultures for G. lloydianum and G. subiculosum collected in the Brazilian Amazon. Data on spore germination, and isolation of monokaryotic cultures and in vitro sexual reproduction are presented, as well as a brief morphological description of the cultures obtained. For both species, Potato Dextrose Agar (PDA) was the most promising of the tested culture media. The highest growth in agar culture ever recorded for this genus is reported (4.9 mm per week for G. lloydianum and 7.5 mm for G. subiculosum). In the PDA culture medium, spores germinated after 35-40 days of incubation and the isolation of monokaryotic cultures of the two species, as well as in vitro sexual crosses, were successfully performed.

scholarly journals Effect of Fungicides on Colony Growth of Colletotrichum Lindemuthianum (Sacc. & Magn.) Scrib.

2008 ◽  
Vol 48 (2) ◽  
pp. 201-212
Author(s):  
Juliana Sartori ◽  
Antonio Maringoni
Keyword(s):  
Culture Medium ◽  
High Sensitivity ◽  
Colony Growth ◽  
Potato Dextrose Agar ◽  
Colletotrichum Lindemuthianum ◽  
Cross Resistance ◽  
Agar Culture ◽  
Thiophanate Methyl ◽  
Low Sensitivity

Effect of Fungicides on Colony Growth ofColletotrichum Lindemuthianum(Sacc. & Magn.) Scrib.Colletotrichum lindemuthianum(Sacc. & Magn.) Scrib. is the causal agent of the anthracnose of common bean (Phaseolus vulgarisL.), a fungal disease of a great significance in brazilian bean cultures. The goals of this work were to evaluate thein vitrocolony growth and to determine the ED50interval of twentyC. lindemuthianumisolates from different regions of Brazil to five fungicides of different active ingredients and to some blendings (carbendazim, chlorothalonil, thiophanate-methyl, chlorothalonil + thiophanate-methyl, trifloxystrobin, propiconazole and trifloxystrobin + propiconazole), at concentrations of 0, 1, 10, 100 and 1000 μg/ml, in a potato-dextrose-agar culture medium. The results revealed seven isolates with low sensitivity to carbendazim and thiophanate-methyl (ED50interval greater than 1000 μg/ml) thus suggesting cross-resistance. Isolate sensitivity to chlorothalonil ranged from ED50interval less than 1 μg/ml to greater than 1000 μg/ml. Those isolates with high sensitivity to thiophanate-methyl, ED50interval less than 1 μg/ml, did also show it with respect to chlorothalonil + thiophanate-methyl. Sixteen isolates showed a high sensitivity to trifloxystrobin with a ED50interval less than 1 μg/ml. Nineteen isolates ofC. lindemuthianumshowed high sensitivity to propiconazole and to trifloxystrobin + propiconazole with ED50interval less than 1 μg/ml. Isolates with low sensitivity to carbendazim and thiophanate-methyl were sensitive to propiconazole and to trifloxystrobin + propiconazole. Variability was found in the sensitivity of the colony growth ofC. lindemuthianumisolates from different regions of Brazil to the fungicides evaluated.

scholarly journals In vitro assay on homeopathic solutions on Metarhizium anisopliae (Metsch) Sorok (Ascomycota: Clavicipitaceae)

2016 ◽  
Vol 82 (0) ◽  
Author(s):  
Silvana Damin ◽  
Luis Francisco Angeli Alves ◽  
Andreia Kusumota Bonini ◽  
Talita Moretto Alexandre
Keyword(s):  
Spodoptera Frugiperda ◽  
Metarhizium Anisopliae ◽  
Culture Medium ◽  
In Vitro Assay ◽  
Distilled Water ◽  
Biological Parameters ◽  
Vitro Assay ◽  
Colony Forming Units ◽  
Vitro Effect

ABSTRACT: This study aimed to evaluate the in vitro effect of homeopathic solutions Arsenicum Album 24CH; Calcarea carbonica 30CH; Kali iodatum 100CH;Phosphorus 3CH; Silicea 30CH;Staphysagria 6, 30 and 100CH; Spodoptera frugiperda 30CH; Sulphur 100 and 200CH andThuya occidentalis 200CH on biological parameters of the fungus Metarhizium anisopliae . The solutions were diluted in sterile distilled water (0.1%) and were sprayed on the previously inoculated fungus on PDA culture medium. Germination, colony forming units, vegetative growth, conidial production and insecticidal activity of the fungus against larvae of Diatraea saccharalis (Lepidoptera: Crambidae) were evaluated. Homeopathic solutions did not affect negatively the parameters evaluated. Thus, all treatments were considered compatible to the fungusM. anisopliae.

scholarly journals In vitro effect of experimental nanocomposites solutions on the prevention of dental caries around orthodontic brackets

2021 ◽  
Vol 32 (4) ◽  
pp. 62-73
Author(s):  
Karla Lorene de França Leite ◽  
Thiago Isidro Vieira ◽  
Adílis Kalina Alexandria ◽  
Raphael Ferreira da Silva ◽  
Aline Saddock de Sá Silva ◽  
...  
Keyword(s):  
Dental Caries ◽  
Culture Medium ◽  
Fluoride Concentration ◽  
Polarized Light ◽  
Sem Analysis ◽  
Orthodontic Brackets ◽  
Titanium Tetrafluoride ◽  
Vitro Effect ◽  
Mineral Loss

Abstract This study aimed to evaluate the in vitro effect of a single application of experimental nanocomposite solutions on the prevention of dental caries around orthodontic brackets. The specimens were exposed to mesoporous silica (MS) nanocomposites containing fluoride by association with titanium tetrafluoride (TiF4) or sodium fluoride (NaF). Nanocomposites also could contain calcium and groups were described as MSCaTiF4, MSTiF4, MSCaNaF, MSNaF, and controls (TiF4, and NaF). Specimens were subjected to the formation of a multispecies biofilm to generate a cariogenic challenge. After 24h, both pH and total soluble fluoride concentration of the culture medium were assessed. Mineral loss was evaluated by percentage of surface mineral loss (%SML), mineral volume variation (ΔZ) of inner enamel and polarized light microscopy (PL). Linear (Ra) and volumetric (Sa) surface roughness and scanning electronic microscopy (SEM) were used to assess enamel topography. Statistical analyses were conducted considering p<0.05. MSNaF had the highest value of culture medium pH after cariogenic challenge, similarly to MSTiF4. All nanocomposite solutions released less fluoride than their controls NaF and TiF4 (p<0.05). All nanocomposite solutions presented lower %SML compared to their respective control groups (p<0.05). Lower Ra, Sa and ΔZ were observed for experimental groups compared to TiF4 (p<0.05). The results were confirmed by PL and SEM analysis. The experimental nanocomposite solutions contributed for lower enamel demineralization around orthodontic brackets.

scholarly journals Cell-cycle manipulation of human leukemic progenitor cells with humoral adjustment in vitro

Blood ◽  
1981 ◽  
Vol 58 (1) ◽  
pp. 179-182 ◽  
Author(s):  
CH Park
Keyword(s):  
Cell Cycle ◽  
Progenitor Cells ◽  
Conditioned Medium ◽  
Culture Medium ◽  
Culture Method ◽  
Leukemic Cells ◽  
Agar Culture ◽  
Daily Feeding

Abstract The cell-cycle change of human leukemic colony-forming cells was studied using a new agar culture method featuring daily feeding of new culture medium with or without leukocyte conditioned medium (LCM). Leukemic cells could be kept out of cycle by withholding LCM from daily feeding and put back into cycle by adding LCM to the daily feeding.

scholarly journals Antibacterial Activity Test of Red Ginger Extract (Zingiber officinale var. Rubrum) Against Streptococcus pyogenes In vitro

2020 ◽  
Vol 3 (1) ◽  
pp. 24
Author(s):  
Samira Assegaf ◽  
Arthur Pohan Kawilarang ◽  
Retno Handajani
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Zingiber Officinale ◽  
Positive Control ◽  
Bacterial Suspension ◽  
Ginger Extract ◽  
Mueller Hinton ◽  
Red Ginger ◽  
Mueller Hinton Broth

Introduction: Red ginger (Zingiber officinale var. rubrum) is known as an antibacterial agent. Streptococcus pyogenes is the most common bacterial cause of pharyngitis in children and adults. Over the years, antibiotic resistance case is increasing. Therefore, further research of other substances to find an bactericidal or bacteriostatic agent is needed. The aim of this study is to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of Z. officinale var. rubrum against S. pyogenes. Methods: Serial dilution test of red ginger extract in Mueller hinton broth (8 concentrations of red ginger extract : 80%, 40%, 20%, 10%, 5%, 2,5%, 1,25%, and 0,625%) and positive control (1 ml bacterial suspension in Mueller hinton broth) was used to determine MIC. MBC was determined by culturing solutions from the previous dilution test into blood agar plate.lResults: MIC of red ginger extract against S. pyogenes could not be determined because the color of red ginger extract affected the turbidity of the dilution test result. MBC of red ginger extract against S. pyogenes was at concentration 20% of red ginger extract.Conclusion: Z. officinale var. rubrum showed antibacterial activity against S. pyogenes.

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