Using density invariant graph Laplacian to resolve unobservable parameters for three-dimensional optical bio-imaging

Author(s):  
Chien-Hung Lu ◽  
Pei-Yuan Wu
2020 ◽  
Vol 117 (27) ◽  
pp. 15403-15408
Author(s):  
Lawrence K. Saul

We propose a latent variable model to discover faithful low-dimensional representations of high-dimensional data. The model computes a low-dimensional embedding that aims to preserve neighborhood relationships encoded by a sparse graph. The model both leverages and extends current leading approaches to this problem. Like t-distributed Stochastic Neighborhood Embedding, the model can produce two- and three-dimensional embeddings for visualization, but it can also learn higher-dimensional embeddings for other uses. Like LargeVis and Uniform Manifold Approximation and Projection, the model produces embeddings by balancing two goals—pulling nearby examples closer together and pushing distant examples further apart. Unlike these approaches, however, the latent variables in our model provide additional structure that can be exploited for learning. We derive an Expectation–Maximization procedure with closed-form updates that monotonically improve the model’s likelihood: In this procedure, embeddings are iteratively adapted by solving sparse, diagonally dominant systems of linear equations that arise from a discrete graph Laplacian. For large problems, we also develop an approximate coarse-graining procedure that avoids the need for negative sampling of nonadjacent nodes in the graph. We demonstrate the model’s effectiveness on datasets of images and text.


Metallomics ◽  
2010 ◽  
Vol 2 (11) ◽  
pp. 745 ◽  
Author(s):  
Dominic J. Hare ◽  
Jessica L. George ◽  
Rudolph Grimm ◽  
Simon Wilkins ◽  
Paul A. Adlard ◽  
...  

2006 ◽  
Vol 46 (supplement2) ◽  
pp. S430
Author(s):  
Tatsunori Goto ◽  
Rina Nagai ◽  
Junichi Takagi ◽  
Kenji Iwasaki ◽  
Takuo Yasunaga

2006 ◽  
Vol 46 (supplement2) ◽  
pp. S424
Author(s):  
Kana Mizutani ◽  
Kazuhiro Oiwa ◽  
Hitoshi Sakakibara ◽  
Tomoko Masaike ◽  
Takayuki Nishizaka

2019 ◽  
Vol 215 ◽  
pp. 13002
Author(s):  
Airidas Žukauskas ◽  
Andreas R. Stilling-Andersen ◽  
Xiaolong Zhu ◽  
Anders Kristensen

Conventional three-dimensional optics requires curvature to control the wave front of light thus making it difficult to reduce the size of the optical systems. Furthermore, for correction of optical aberrations, complex optical systems comprising more than one lens are used. This adds additional bulk, mass and complexity to the optical systems. Recent development in diffractive optics has enabled new thin lightweight optical elements such as metalenses. We introduce resonant laser printing technique as a flexible photo-thermal technology for metalens fabrication with the ability to control the light with microscale precision. Our laser printed metalenses can be integrated in bio-sensors, bio-imaging systems, and optofluidical devices.


1966 ◽  
Vol 25 ◽  
pp. 227-229 ◽  
Author(s):  
D. Brouwer

The paper presents a summary of the results obtained by C. J. Cohen and E. C. Hubbard, who established by numerical integration that a resonance relation exists between the orbits of Neptune and Pluto. The problem may be explored further by approximating the motion of Pluto by that of a particle with negligible mass in the three-dimensional (circular) restricted problem. The mass of Pluto and the eccentricity of Neptune's orbit are ignored in this approximation. Significant features of the problem appear to be the presence of two critical arguments and the possibility that the orbit may be related to a periodic orbit of the third kind.


Author(s):  
M. Boublik ◽  
W. Hellmann ◽  
F. Jenkins

The present knowledge of the three-dimensional structure of ribosomes is far too limited to enable a complete understanding of the various roles which ribosomes play in protein biosynthesis. The spatial arrangement of proteins and ribonuclec acids in ribosomes can be analysed in many ways. Determination of binding sites for individual proteins on ribonuclec acid and locations of the mutual positions of proteins on the ribosome using labeling with fluorescent dyes, cross-linking reagents, neutron-diffraction or antibodies against ribosomal proteins seem to be most successful approaches. Structure and function of ribosomes can be correlated be depleting the complete ribosomes of some proteins to the functionally inactive core and by subsequent partial reconstitution in order to regain active ribosomal particles.


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