Mechanism and Methods of Outdoor AR Spatial Information Visualization Representation

Various authors have emphasized the spatial information resident in an electron micrograph taken with adequately coherent radiation. In view of the completion of at least one such instrument, this opportunity is taken to summarize the state of the art of processing such micrographs. We use the usual symbols for the aberration coefficients, and supplement these with £ and 6 for the transverse coherence length and the fractional energy spread respectively. He also assume a weak, biologically interesting sample, with principal interest lying in the molecular skeleton remaining after obvious hydrogen loss and other radiation damage has occurred.

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3-D optical transfer in excitation field synthesis microscopes

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100136696 ◽

1995 ◽

Vol 53 ◽

pp. 64-65

Author(s):

Vijay Krishnamurthi ◽

Brent Bailey ◽

Frederick Lanni

Keyword(s):

Standing Wave ◽

Spatial Information ◽

3D Structure ◽

Complete Recovery ◽

Weighting Factor ◽

Optical Transfer Function ◽

Excitation Field ◽

Optical Transfer ◽

Set Up ◽

Focus Plane

Excitation field synthesis (EFS) refers to the use of an interference optical system in a direct-imaging microscope to improve 3D resolution by axially-selective excitation of fluorescence within a specimen. The excitation field can be thought of as a weighting factor for the point-spread function (PSF) of the microscope, so that the optical transfer function (OTF) gets expanded by convolution with the Fourier transform of the field intensity. The simplest EFS system is the standing-wave fluorescence microscope, in which an axially-periodic excitation field is set up through the specimen by interference of a pair of collimated, coherent, s-polarized beams that enter the specimen from opposite sides at matching angles. In this case, spatial information about the object is recovered in the central OTF passband, plus two symmetric, axially-shifted sidebands. Gaps between these bands represent "lost" information about the 3D structure of the object. Because the sideband shift is equal to the spatial frequency of the standing-wave (SW) field, more complete recovery of information is possible by superposition of fields having different periods. When all of the fields have an antinode at a common plane (set to be coincident with the in-focus plane), the "synthesized" field is peaked in a narrow infocus zone.

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Use of EDS mapping to characterize thermochemical stability of fibers in intermetallic matrix composites

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010012120x ◽

1992 ◽

Vol 50 (1) ◽

pp. 160-161

Author(s):

John R. Porter

Keyword(s):

Spatial Information ◽

Beam Current ◽

Fiber Types ◽

Ceramic Fibers ◽

Dye Transfer ◽

Science Center ◽

Commercial Development ◽

Nih Image ◽

Intermetallic Matrix Composites ◽

Thermochemical Stability

New ceramic fibers, currently in various stages of commercial development, have been consolidated in intermetallic matrices such as γ-TiAl and FeAl. Fiber types include SiC, TiB2 and polycrystalline and single crystal Al2O3. This work required the development of techniques to characterize the thermochemical stability of these fibers in different matrices.SEM/EDS elemental mapping was used for this work. To obtain qualitative compositional/spatial information, the best realistically achievable counting statistics were required. We established that 128 × 128 maps, acquired with a 20 KeV accelerating voltage, 3 sec. live time per pixel (total mapping time, 18 h) and with beam current adjusted to give 30% dead time, provided adequate image quality at a magnification of 800X. The maps were acquired, with backgrounds subtracted, using a Noran TN 5500 EDS system. The images and maps were transferred to a Macintosh and converted into TIFF files using either TIFF Maker, or TNtolMAGE, a Microsoft QuickBASIC program developed at the Science Center. From TIFF files, images and maps were opened in either NIH Image or Adobe Photoshop for processing and analysis and printed from Microsoft Powerpoint on a Kodak XL7700 dye transfer image printer.

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