Mobile unit velocity estimation in micro-cellular systems based on the statistical characteristics of the received signal

Author(s):  
Zhang Hao ◽  
Liu Yong ◽  
Gao Junxiang
2020 ◽  
Author(s):  
Nikolas Hundt

Abstract Single-molecule imaging has mostly been restricted to the use of fluorescence labelling as a contrast mechanism due to its superior ability to visualise molecules of interest on top of an overwhelming background of other molecules. Recently, interferometric scattering (iSCAT) microscopy has demonstrated the detection and imaging of single biomolecules based on light scattering without the need for fluorescent labels. Significant improvements in measurement sensitivity combined with a dependence of scattering signal on object size have led to the development of mass photometry, a technique that measures the mass of individual molecules and thereby determines mass distributions of biomolecule samples in solution. The experimental simplicity of mass photometry makes it a powerful tool to analyse biomolecular equilibria quantitatively with low sample consumption within minutes. When used for label-free imaging of reconstituted or cellular systems, the strict size-dependence of the iSCAT signal enables quantitative measurements of processes at size scales reaching from single-molecule observations during complex assembly up to mesoscopic dynamics of cellular components and extracellular protrusions. In this review, I would like to introduce the principles of this emerging imaging technology and discuss examples that show how mass-sensitive iSCAT can be used as a strong complement to other routine techniques in biochemistry.


2005 ◽  
Vol 10 (2) ◽  
pp. 151-160 ◽  
Author(s):  
J. Kala ◽  
Z. Kala

Authors of article analysed influence of variability of yield strength over cross-section of hot rolled steel member to its load-carrying capacity. In calculation models, the yield strength is usually taken as constant. But yield strength of a steel hot-rolled beam is generally a random quantity. Not only the whole beam but also its parts have slightly different material characteristics. According to the results of more accurate measurements, the statistical characteristics of the material taken from various cross-section points (e.g. from a web and a flange) are, however, more or less different. This variation is described by one dimensional random field. The load-carrying capacity of the beam IPE300 under bending moment at its ends with the lateral buckling influence included is analysed, nondimensional slenderness according to EC3 is λ¯ = 0.6. For this relatively low slender beam the influence of the yield strength on the load-carrying capacity is large. Also the influence of all the other imperfections as accurately as possible, the load-carrying capacity was determined by geometrically and materially nonlinear solution of very accurate FEM model by the ANSYS programme.


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