scholarly journals Optical Fiber Refractive Index Profiling by Iterative Optical Diffraction Tomography

2018 ◽  
Vol 36 (24) ◽  
pp. 5754-5763 ◽  
Author(s):  
Shengli Fan ◽  
Seth Smith-Dryden ◽  
Jian Zhao ◽  
Stefan Gausmann ◽  
Axel Schulzgen ◽  
...  
Keyword(s):  
Refractive Index ◽  
Optical Fiber ◽  
Optical Diffraction ◽  
Diffraction Tomography ◽  
Optical Diffraction Tomography

Enhancement of optical resolution in three-dimensional refractive-index tomograms of biological samples by employing micromirror-embedded coverslips

Lab on a Chip ◽  
2018 ◽  
Vol 18 (22) ◽  
pp. 3484-3491 ◽  
Author(s):  
Seungwoo Shin ◽  
Jihye Kim ◽  
Je-Ryung Lee ◽  
Eun-chae Jeon ◽  
Tae-Jin Je ◽  
...  
Keyword(s):  
Refractive Index ◽  
Biological Samples ◽  
Three Dimensional ◽  
Optical Resolution ◽  
Optical Diffraction ◽  
Diffraction Tomography ◽  
Optical Diffraction Tomography

Resolution-enhanced optical diffraction tomography using a micromirror-embedded coverslips.

scholarly journals Three-dimensional refractive index distributions of individual angiosperm pollen grains

2018 ◽  
Author(s):  
Chansuk Park ◽  
SangYun Lee ◽  
Geon Kim ◽  
SeungJun Lee ◽  
Jaehoon Lee ◽  
...  
Keyword(s):  
Refractive Index ◽  
Three Dimensional ◽  
Pollen Grains ◽  
Optical Diffraction ◽  
Diffraction Tomography ◽  
Optical Diffraction Tomography ◽  
Internal Structures ◽  
Quantitative Analyses ◽  
Angiosperm Pollen ◽  
Preparation Techniques

Three-dimensional (3D) refractive index (RI) imaging and quantitative analyses of angiosperm pollen grains are presented. Using optical diffraction tomography, the 3D RI structures of individual angiosperm pollen grains were measured without using labeling or other preparation techniques. Various physical quantities, including volume, surface area, exine volume, and sphericity, were determined from the measured RI tomograms of pollen grains. Exine skeletons, the distinct internal structures of angiosperm pollen grains, were identified and systematically analyzed.

Investigation of limitations of optical diffraction tomography

2007 ◽  
Vol 15 (2) ◽  
Author(s):  
T. Kozacki ◽  
M. Kujawińska ◽  
P. Kniażewski
Keyword(s):  
Refractive Index ◽  
Dynamic Range ◽  
Tomographic Reconstruction ◽  
Back Propagation ◽  
Reconstruction Algorithm ◽  
Optical Diffraction ◽  
Diffraction Tomography ◽  
Refractive Index Distribution ◽  
Optical Diffraction Tomography ◽  
Index Distribution

AbstractOptical diffraction tomography (ODT) applied to measurement of optical microelements is limited by low dynamic range, i.e., only objects with small deviations of refractive-index distribution can be measured. Therefore in this paper the limitations and errors of ODT are investigated throughout extensive numerical experiments. It is shown that these errors can be reduced by introduction of additional numerical focusing in the tomographic reconstruction algorithm. Additionally, new tomographic reconstruction algorithm using back propagation in reference medium for optical microelements measurement with known design is proposed. This hybrid reconstruction algorithm allows significant extension of ODT applicability in measurement of elements having large deviations of refractive-index distribution.

scholarly journals 3D label-free imaging and analysis of Pinus pollen grains using optical diffraction tomography

2017 ◽  
Author(s):  
Geon Kim ◽  
SangYun Lee ◽  
Seungwoo Shin ◽  
YongKeun Park
Keyword(s):  
Refractive Index ◽  
Three Dimensional ◽  
Pollen Grains ◽  
Reproductive Function ◽  
Optical Diffraction ◽  
List Type ◽  
Diffraction Tomography ◽  
Label Free ◽  
Optical Diffraction Tomography ◽  
Index Maps

SummaryThe structure of pollen grains is related to the reproductive function of the plants. Here, three-dimensional (3D) refractive index maps were obtained for individual conifer pollen grains using optical diffraction tomography (ODT).The 3D morphological features of pollen grains from pine trees were investigated using measured refractive index maps, in which distinct substructures were clearly distinguished and analyzed.Morphological and physiochemical parameters of the pollen grains were quantified from the obtained refractive index (RI) maps and used to quantitatively study the interspecific differences of pollen grains from different strains.Our results demonstrate that ODT can assess the structure of pollen grains. This label-free and rapid 3D imaging approach may provide a new platform for understanding the physiology of pollen grains.

scholarly journals Label-free high-resolution 3-D imaging of gold nanoparticles inside live cells using optical diffraction tomography

2016 ◽  
Author(s):  
Doyeon Kim ◽  
Nuri Oh ◽  
Kyoohyun Kim ◽  
SangYun Lee ◽  
Chan-Gi Pack ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Refractive Index ◽  
Quantitative Imaging ◽  
Extended Period ◽  
Optical Diffraction ◽  
Live Cells ◽  
Diffraction Tomography ◽  
Label Free ◽  
Optical Diffraction Tomography ◽  
4T1 Cells

AbstractDelivery of gold nanoparticles (GNPs) into live cells has high potentials, ranging from molecular-specific imaging, photodiagnostics, to photothermal therapy. However, studying the long-term dynamics of cells with GNPs using conventional fluorescence techniques suffers from phototoxicity and photobleaching. Here, we present a method for 3-D imaging of GNPs inside live cells exploiting refractive index (RI) as imaging contrast. Employing optical diffraction tomography, 3-D RI tomograms of live cells with GNPs are precisely measured for an extended period with sub-micrometer resolution. The locations and contents of GNPs in live cells are precisely addressed and quantified due to their distinctly high RI values, which was validated by confocal fluorescence imaging of fluorescent dye conjugated GNPs. In addition, we perform quantitative imaging analysis including the segmentations of GNPs in the cytosol, the volume distributions of aggregated GNPs, and the temporal evolution of GNPs contents in HeLa and 4T1 cells.AbbreviationsGNPsgold nanoparticlesRIrefractive indexODToptical diffraction tomographyDMDdigital micromirror device

scholarly journals Intracellular Detection and Localization of Nanoparticles by Refractive Index Measurement

Sensors ◽  
2021 ◽  
Vol 21 (15) ◽  
pp. 5001
Author(s):  
Alain Géloën ◽  
Karyna Isaieva ◽  
Mykola Isaiev ◽  
Olga Levinson ◽  
Emmanuelle Berger ◽  
...  
Keyword(s):  
Refractive Index ◽  
Optical Diffraction ◽  
Live Cells ◽  
Diffraction Tomography ◽  
Refractive Index Measurement ◽  
Optical Diffraction Tomography ◽  
Fluorescence Measurements ◽  
Index Measurement ◽  
Intracellular Detection

The measuring of nanoparticle toxicity faces an important limitation since it is based on metrics exposure, the concentration at which cells are exposed instead the true concentration inside the cells. In vitro studies of nanomaterials would benefit from the direct measuring of the true intracellular dose of nanoparticles. The objective of the present study was to state whether the intracellular detection of nanodiamonds is possible by measuring the refractive index. Based on optical diffraction tomography of treated live cells, the results show that unlabeled nanoparticles can be detected and localized inside cells. The results were confirmed by fluorescence measurements. Optical diffraction tomography paves the way to measuring the true intracellular concentrations and the localization of nanoparticles which will improve the dose-response paradigm of pharmacology and toxicology in the field of nanomaterials.

Sign in / Sign up

Export Citation Format

Share Document