Synthetic database for testing algorithms of fetal ECG extraction from abdominal ECG

2012 ◽  
Author(s):  
Luis Omar Sarmiento Alvarez ◽  
Alberto Gonzalez ◽  
Jose Millet
Keyword(s):  
Fetal Ecg ◽  
Testing Algorithms

FETAL ECG EXTRACTION USING WAVELET AND ADAPTIVE FILTERING TECHNIQUES

2016 ◽  
Vol 4 (2) ◽  
pp. 1
Author(s):  
PRASAD K. PURUSHOTHAM ◽  
ANURADHA B. ◽  
◽  
Keyword(s):  
Adaptive Filtering ◽  
Fetal Ecg ◽  
Filtering Techniques

Ambulatory fetal ECG monitoring

2017 ◽  
Vol 12 (13) ◽  
pp. 75-82
Author(s):  
K.V. Nasedkin ◽  
◽  
V.V. Fedotenko ◽  
O.G. Viunytskyi ◽  
V.I. Shulgin ◽  
...  
Keyword(s):  
Ecg Monitoring ◽  
Fetal Ecg

scholarly journals Assessment of common housekeeping genes as reference for gene expression studies using RT-qPCR in mouse choroid plexus

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kim Hoa Ho ◽  
Annarita Patrizi
Keyword(s):  
Gene Expression ◽  
Choroid Plexus ◽  
Sensory Deprivation ◽  
Housekeeping Genes ◽  
Housekeeping Gene ◽  
Experimental Conditions ◽  
Brain Repair ◽  
Expression Studies ◽  
Testing Algorithms ◽  
Gene Expression Studies

AbstractChoroid plexus (ChP), a vascularized secretory epithelium located in all brain ventricles, plays critical roles in development, homeostasis and brain repair. Reverse transcription quantitative real-time PCR (RT-qPCR) is a popular and useful technique for measuring gene expression changes and also widely used in ChP studies. However, the reliability of RT-qPCR data is strongly dependent on the choice of reference genes, which are supposed to be stable across all samples. In this study, we validated the expression of 12 well established housekeeping genes in ChP in 2 independent experimental paradigms by using popular stability testing algorithms: BestKeeper, DeltaCq, geNorm and NormFinder. Rer1 and Rpl13a were identified as the most stable genes throughout mouse ChP development, while Hprt1 and Rpl27 were the most stable genes across conditions in a mouse sensory deprivation experiment. In addition, Rpl13a, Rpl27 and Tbp were mutually among the top five most stable genes in both experiments. Normalisation of Ttr and Otx2 expression levels using different housekeeping gene combinations demonstrated the profound effect of reference gene choice on target gene expression. Our study emphasized the importance of validating and selecting stable housekeeping genes under specific experimental conditions.

Extraction of fetal ECG a semi-blind source separation algorithm combined with parametrized kalman filter

2021 ◽  
Author(s):  
J. Sakubar Sadiq ◽  
G. Arunmani ◽  
P. Ravivarma ◽  
N. Karthika Devi ◽  
A. Hemalatha ◽  
...  
Keyword(s):  
Kalman Filter ◽  
Blind Source Separation ◽  
Source Separation ◽  
Separation Algorithm ◽  
Fetal Ecg

Current Developments in Diagnostic Assays for Laboratory Confirmation and Investigation of Botulism

2021 ◽  
Author(s):  
Dominick A. Centurioni ◽  
Christina T. Egan ◽  
Michael J. Perry
Keyword(s):  
Gold Standard ◽  
Botulinum Neurotoxin ◽  
Mouse Bioassay ◽  
Environmental Matrices ◽  
Animal Testing ◽  
Gold Standard Method ◽  
Diagnostic Assays ◽  
Technical Performance ◽  
Laboratory Confirmation ◽  
Testing Algorithms

Detection of botulinum neurotoxin or isolation of the toxin producing organism is required for the laboratory confirmation of botulism in clinical specimens. In an effort to reduce animal testing required by the gold standard method of botulinum neurotoxin detection, the mouse bioassay, many technologies have been developed to detect and characterize the causative agent of botulism. Recent advancements in these technologies have led to improvements in technical performance of diagnostic assays; however, many emerging assays have not been validated for the detection of all serotypes in complex clinical and environmental matrices. Improvements to culture protocols, endopeptidase-based assays, and a variety of immunological and molecular methods have provided laboratories with a variety of testing options to evaluate and incorporate into their testing algorithms. While significant advances have been made to improve these assays, additional work is necessary to evaluate these methods in various clinical matrices and to establish standardized criteria for data analysis and interpretation.

Sign in / Sign up

Export Citation Format

Share Document