A novel optical assay system for bilirubin concentration measurement in whole blood

2021 ◽  
pp. 1-1
Author(s):  
Jean Pierre Ndabakuranye ◽  
Anushi E Rajapaksa ◽  
Genia Burchall ◽  
Shiqiang Li ◽  
Steven Prawer ◽  
...  
Keyword(s):  
Whole Blood ◽  
Concentration Measurement ◽  
Assay System ◽  
Bilirubin Concentration ◽  
Optical Assay

Development of a point-of-care assay system for high-sensitivity C-reactive protein in whole blood

2003 ◽  
Vol 332 (1-2) ◽  
pp. 51-59 ◽  
Author(s):  
Jae Soon Ahn ◽  
Sunga Choi ◽  
Sang Ho Jang ◽  
Hyuk Jae Chang ◽  
Jae Hoon Kim ◽  
...  
Keyword(s):  
Whole Blood ◽  
Point Of Care ◽  
High Sensitivity ◽  
Assay System ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Point Of Care Assay

A novel optical assay system for the quantitative measurement of chemotaxis

2003 ◽  
Vol 282 (1-2) ◽  
pp. 1-11 ◽  
Author(s):  
Shiro Kanegasaki ◽  
Yuka Nomura ◽  
Nao Nitta ◽  
Shuichi Akiyama ◽  
Takuya Tamatani ◽  
...  
Keyword(s):  
Quantitative Measurement ◽  
Assay System ◽  
Optical Assay

scholarly journals Passive Coagulability Assay Based on Coherence-Gated Light Scattering

Hemato ◽  
2020 ◽  
Vol 1 (2) ◽  
pp. 49-59
Author(s):  
Mahed Batarseh ◽  
Jose Rafael Guzman-Sepulveda ◽  
Ruitao Wu ◽  
William M. DeCampli ◽  
Aristide Dogariu
Keyword(s):  
Light Scattering ◽  
Whole Blood ◽  
In Vitro Tests ◽  
Clinical Procedures ◽  
Clinical Methods ◽  
Blood Coagulability ◽  
Natural Dynamics ◽  
Optical Assay ◽  
External Stimuli

Coagulation monitoring relies on in vitro tests where the clot formation is induced using external stimuli. We report an optical method capable of revealing the propensity of coagulation based solely on the natural dynamics of erythrocytes in whole blood. In contrast to traditional techniques, our approach provides means to assess the blood coagulability without the need to chemically trigger the coagulation. Results of correlations with standard clinical methods suggest that this optical assay could be used for continuous management of blood coagulation during clinical procedures.

Electrochemical assay system with single-use electrode strip for measuring lactate in whole blood

1993 ◽  
Vol 39 (11) ◽  
pp. 2312-2314 ◽  
Author(s):  
N Shimojo ◽  
K Naka ◽  
H Uenoyama ◽  
K Hamamoto ◽  
K Yoshioka ◽  
...  
Keyword(s):  
Whole Blood ◽  
Enzymatic Reaction ◽  
Lactate Concentration ◽  
Assay System ◽  
Lactate Oxidase ◽  
Electron Mediator ◽  
Electrochemical Assay ◽  
Electrode Strip ◽  
Before And After ◽  
Single Use

Abstract We have developed an assay system for measuring lactate in whole blood, consisting of a single-use strip of an enzyme-coated electrode and a small meter. The electrode strip is made of three plastic films: a cover sheet, a spacer, and an insulation layer printed with electrodes that are coated with lactate oxidase (EC 1.1.3.x) and ferricyanide as an electron mediator. The meter measures the magnitude of the anodic current of the reduced mediator by the enzymatic reaction and displays the lactate concentration 60 s after a blood sample (5 microL) is applied. The calibration curve was linear up to 20 mmol/L, and the between-run CVs at three concentrations were 1.7-8.4%. Lactate concentrations determined by this method (y) in blood samples from healthy individuals before and after exercise agreed with the results obtained by the conventional enzymatic method (x): y = 0.97x - 0.3, Sy/x = 0.7. This assay provides a rapid and convenient test for measuring blood lactate concentrations.

Fluorescent capillary fill device: a quantitative whole-blood assay system suited to point-of-care use

1998 ◽  
Author(s):  
Phelim B. Daniels ◽  
J. Philip Vessey ◽  
Janys E. Fletcher ◽  
Paul M. O'Neill ◽  
Christopher G. Stafford ◽  
...  
Keyword(s):  
Whole Blood ◽  
Point Of Care ◽  
Assay System ◽  
Whole Blood Assay ◽  
Blood Assay

Evaluation of Total Bilirubin Concentration Measurement in CO-oximeter

2008 ◽  
Vol 78 (7) ◽  
pp. 427-432
Author(s):  
Takashi Makaya ◽  
Daisuke Yamashita ◽  
Gaku Sugihara ◽  
Hideki Kojima ◽  
Shigeru Sano ◽  
...  
Keyword(s):  
Total Bilirubin ◽  
Concentration Measurement ◽  
Bilirubin Concentration ◽  
Total Bilirubin Concentration

Fine Structure of Platelet Interaction with a New Microcrystalline Collagen Preparation

1974 ◽  
Vol 32 ◽  
pp. 58-59
Author(s):  
W. H. Zucker ◽  
R. G. Mason
Keyword(s):  
Fine Structure ◽  
Platelet Aggregation ◽  
Hydrochloric Acid ◽  
Whole Blood ◽  
Platelet Adhesion ◽  
Hemostatic Agent ◽  
Native Collagen ◽  
Fibrillar Morphology ◽  
Clinical Interest ◽  
New Form

Platelet adhesion initiates platelet aggregation and is an important component of the hemostatic process. Since the development of a new form of collagen as a topical hemostatic agent is of both basic and clinical interest, an ultrastructural and hematologic study of the interaction of platelets with the microcrystalline collagen preparation was undertaken.In this study, whole blood anticoagulated with EDTA was used in order to inhibit aggregation and permit study of platelet adhesion to collagen as an isolated event. The microcrystalline collagen was prepared from bovine dermal corium; milling was with sharp blades. The preparation consists of partial hydrochloric acid amine collagen salts and retains much of the fibrillar morphology of native collagen.

3-D organization of fibrinogen receptors using computer reconstruction and whole-mount microscopy

1988 ◽  
Vol 46 ◽  
pp. 30-31
Author(s):  
E. T. O'Toole ◽  
R. R. Hantgan ◽  
J. C. Lewis
Keyword(s):  
Whole Blood ◽  
Colloidal Gold ◽  
Blood Platelets ◽  
Cell Contact ◽  
Computer Assisted ◽  
Adherent Cells ◽  
Differential Centrifugation ◽  
Computer Reconstruction ◽  
Sds Page ◽  
Whole Mount

Thrombocytes (TC), the avian equivalent of blood platelets, support hemostasis by aggregating at sites of injury. Studies in our lab suggested that fibrinogen (fib) is a requisite cofactor for TC aggregation but operates by an undefined mechanism. To study the interaction of fib with TC and to identify fib receptors on cells, fib was purified from pigeon plasma, conjugated to colloidal gold and used both to facilitate aggregation and as a receptor probe. Described is the application of computer assisted reconstruction and stereo whole mount microscopy to visualize the 3-D organization of fib receptors at sites of cell contact in TC aggregates and on adherent cells.Pigeon TC were obtained from citrated whole blood by differential centrifugation, washed with Ca++ free Hank's balanced salts containing 0.3% EDTA (pH 6.5) and resuspended in Ca++ free Hank's. Pigeon fib was isolated by precipitation with PEG-1000 and the purity assessed by SDS-PAGE. Fib was conjugated to 25nm colloidal gold by vortexing and the conjugates used as the ligand to identify fib receptors.

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