In Vivo Monitoring of Orthopaedic Implant Wear Using Amorphous Ribbons

2015 ◽  
Vol 51 (1) ◽  
pp. 1-3
Author(s):  
David Okhiria ◽  
Dia E. Giebaly ◽  
Turgut Meydan ◽  
Samuel Bigot ◽  
Peter Theobald
Keyword(s):  
Orthopaedic Implant ◽  
Amorphous Ribbons ◽  
Implant Wear

scholarly journals Local delivery of mutant CCL2 protein-reduced orthopaedic implant wear particle-induced osteolysis and inflammation in vivo

2015 ◽  
Vol 34 (1) ◽  
pp. 58-64 ◽  
Author(s):  
Xinyi Jiang ◽  
Taishi Sato ◽  
Zhenyu Yao ◽  
Michael Keeney ◽  
Jukka Pajarinen ◽  
...  
Keyword(s):  
Wear Particle ◽  
Local Delivery ◽  
Orthopaedic Implant ◽  
Implant Wear

scholarly journals A simple method of suspending implant wear particles for more physiological modelling of cell-particle interactions in vitro

2021 ◽  
Author(s):  
Christine Poon
Keyword(s):  
Wear Debris ◽  
Culture Media ◽  
Polymeric Materials ◽  
Wear Particle ◽  
Physicochemical Characteristics ◽  
Wear Particles ◽  
Simple Method ◽  
Implant Wear

AbstractArthroplasty implants e.g. hip, knee, spinal disc sustain relatively high compressive loading and friction wear, which lead to the formation of wear particles or debris between articulating surfaces. Despite advances in orthopaedic materials and surface treatments, the production of wear debris from any part of a joint arthroplasty implant is currently unavoidable. Implant wear debris induces host immune responses and inflammation, which causes patient pain and ultimately implant failure through progressive inflammation-mediated osteolysis and implant loosening, where the severity and rate of periprosthetic osteolysis depends on the material and physicochemical characteristics of the wear particles. Evaluating the cytotoxicity of implant wear particles is important for regulatory approved clinical application of arthroplasty implants, as is the study of cell-particle response pathways. However, the wear particles of polymeric materials commonly used for arthroplasty implants tend to float when placed in culture media, which limits their contact with cell cultures. This study reports a simple means of suspending wear particles in liquid medium using sodium carboxymethyl cellulose (NaCMC) to provide a more realistic proxy of the interaction between cells and tissues to wear particles in vivo, which are free-floating in synovial fluid within the joint cavity. Low concentrations of NaCMC dissolved in culture medium were found to be effective for suspending polymeric wear particles. Such suspensions may be used as more physiologically-relevant means for testing cellular responses to implant wear debris, as well as studying the combinative effects of shear and wear particle abrasion on cells in a dynamic culture environments such as perfused tissue-on-chip devices.

scholarly journals Blood titanium level as a biomarker of orthopaedic implant wear

2019 ◽  
Vol 53 ◽  
pp. 120-128 ◽  
Author(s):  
Ilona Swiatkowska ◽  
Nicholas Martin ◽  
Alister J. Hart
Keyword(s):  
Orthopaedic Implant ◽  
Implant Wear

Factors contributing to orthopaedic implant wear

2013 ◽  
pp. 310-350
Author(s):  
L.C. Jones ◽  
A.K. Tsao ◽  
L.D.T. Topoleski
Keyword(s):  
Orthopaedic Implant ◽  
Implant Wear

scholarly journals A biomechanical study of the effects of simulated ulnar deviation on silicone finger joint implant failure

2016 ◽  
Vol 41 (9) ◽  
pp. 944-947 ◽  
Author(s):  
P. Drayton ◽  
B. W. Morgan ◽  
M. C. Davies ◽  
G. E. B. Giddins ◽  
A. W. Miles
Keyword(s):  
Metacarpophalangeal Joint ◽  
Biomechanical Study ◽  
Implant Failure ◽  
Level Of Evidence ◽  
Ulnar Deviation ◽  
Ringer’S Solution ◽  
Joint Implant ◽  
Cause Of Failure ◽  
Implant Wear

Silicone finger arthroplasties are used widely, especially for metacarpophalangeal joint replacement in patients with inflammatory arthritis. Implant failure is well recognized. The rates of failure in vivo differ substantially from experience in vivo. One cause of failure is felt to be post-operative ulnar deviation. The aim of our study was to test the effect of ulnar deviation testing on silicone finger implants. We tested 12 implants in three groups of four implants. The implants were submerged in a bath of Ringer’s solution at 370 °C throughout the experiment and tested in a rig held in 0°, 10° and 20° deviation. The rig was cycled at 1.5 Hz from 0°–90°. The implants were inspected every 500,000 cycles until a total of 4 million cycles. There was consistently increased wear and supination plastic deformity in going from 0°–20° deviation. This study confirms the adverse effects of ulnar deviation on silicone finger implant wear. It is likely that this combines with lateral pinch forces and sharp bone edges to cause catastrophic silicone implant failure. Level of evidence: III

scholarly journals Development of an Anti-infective Coating on the Surface of Intraosseous Implants Responsive to Enzymes and Bacteria

2021 ◽  
Author(s):  
Xin Liao ◽  
Xingfang Yu ◽  
Haiping Yu ◽  
Jiaqi Huang ◽  
Bi Zhang ◽  
...  
Keyword(s):  
Multilayer Films ◽  
Orthopaedic Implant ◽  
Inhibition Rate ◽  
Biofilm Inhibition ◽  
Bacterial Inhibition ◽  
Activity Effect ◽  
K Wires ◽  
Substrate Independent

Abstract BackgroundBacterial proliferation on the endosseous implants surface presents a new threat to the using of the bone implants. Unfortunately, there is no effective constructed antibacterial coating which is bacterial anti-adhesion substrate-independent or have long-term biofilm inhibition functions. MethodsDrug release effect was tested in CMS solution and S. aureus. We used bacterial inhibition rate assays and protein leakageexperiment to analyze the in vitro antibacterial effect of (MMT/PLL-CHX)10 multilayer film. We used the CCK-8 assay to analyze the effect of (MMT/PLL-CHX)10 multilayer films on the growth and proliferation of rat osteoblasts. Rat orthopaedic implant-related infections model was constructed to test the antimicrobial activity effect of (MMT/PLL-CHX)10 multilayer films in vivo.ResultsIn this study, the (MMT/PLL-CHX)10 multilayer films structure were progressively degraded and showed well concentration-dependent degradation characteristics following incubation with Staphylococcus aureus and CMS solution. Bacterial inhibition rate assays and protein leakageexperiment showed high levels of bactericidal activity. While the CCK-8 analysis proved that the (MMT/PLL-CHX)10 multilayer films possess perfect biocompatibility. It is somewhat encouraging that in the in vivo antibacterial tests, the K-wires coated with (MMT/PLL-CHX)10 multilayer films showed lower infections incidence and inflammation than the unmodified group, and all parameters are close to SHAM group. Conclusion(MMT/PLL-CHX)10 multilayer films provides a potential therapeutic method for orthopaedic implant-related infections.

scholarly journals A Bone-Targeting Enoxacin Delivery System to Eradicate Staphylococcus Aureus-Related Implantation Infections and Bone Loss

2021 ◽  
Vol 9 ◽  
Author(s):  
Cong Yao ◽  
Meisong Zhu ◽  
Xiuguo Han ◽  
Qiang Xu ◽  
Min Dai ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Bone Loss ◽  
Bone Tissue ◽  
Mesoporous Silica Nanoparticles ◽  
Osteoclast Differentiation ◽  
Osteoclast Formation ◽  
Bacterial Biofilm ◽  
Orthopaedic Implant

Post-operative infections in orthopaedic implants are severe complications that require urgent solutions. Although conventional antibiotics limit bacterial biofilm formation, they ignore the bone loss caused by osteoclast formation during post-operative orthopaedic implant-related infections. Fortunately, enoxacin exerts both antibacterial and osteoclast inhibitory effects, playing a role in limiting infection and preventing bone loss. However, enoxacin lacks specificity in bone tissue and low bioavailability-related adverse effects, which hinders translational practice. Here, we developed a nanosystem (Eno@MSN-D) based on enoxacin (Eno)-loaded mesoporous silica nanoparticles (MSN), decorated with the eight repeating sequences of aspartate (D-Asp8), and coated with polyethylene glycol The release results suggested that Eno@MSN-D exhibits a high sensitivity to acidic environment. Moreover, this Eno@MSN-D delivery nanosystem exhibited both antibacterial and anti-osteoclast properties in vitro. The cytotoxicity assay revealed no cytotoxicity at the low concentration (20 μg/ml) and Eno@MSN-D inhibited RANKL-induced osteoclast differentiation. Importantly, Eno@MSN-D allowed the targeted release of enoxacin in infected bone tissue. Bone morphometric analysis and histopathology assays demonstrated that Eno@MSN-D has antibacterial and antiosteoclastic effects in vivo, thereby preventing implant-related infections and bone loss. Overall, our study highlights the significance of novel biomaterials that offer new alternatives to treat and prevent orthopaedic Staphylococcus aureus-related implantation infections and bone loss.

scholarly journals Predilection for developing a hematogenous orthopaedic implant-associated infection in older versus younger mice

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
John M. Thompson ◽  
Alyssa G. Ashbaugh ◽  
Yu Wang ◽  
Robert J. Miller ◽  
Julie E. Pickett ◽  
...  
Keyword(s):  
At Risk ◽  
Staphylococcus Aureus ◽  
Mouse Model ◽  
Ex Vivo ◽  
Kirschner Wire ◽  
Age Groups ◽  
Orthopaedic Implant ◽  
X Ray ◽  
Risk Patients

Abstract Background The pathogenesis of hematogenous orthopaedic implant-associated infections (HOIAI) remains largely unknown, with little understanding of the influence of the physis on bacterial seeding. Since the growth velocity in the physis of long bones decreases during aging, we sought to evaluate the role of the physis on influencing the development of Staphylococcus aureus HOIAI in a mouse model comparing younger versus older mice. Methods In a mouse model of HOIAI, a sterile Kirschner wire was inserted retrograde into the distal femur of younger (5–8-week-old) and older (14–21-week-old) mice. After a 3-week convalescent period, a bioluminescent Staphylococcus aureus strain was inoculated intravenously. Bacterial dissemination to operative and non-operative legs was monitored longitudinally in vivo for 4 weeks, followed by ex vivo bacterial enumeration and X-ray analysis. Results In vivo bioluminescence imaging and ex vivo CFU enumeration of the bone/joint tissue demonstrated that older mice had a strong predilection for developing a hematogenous infection in the operative legs but not the non-operative legs. In contrast, this predilection was less apparent in younger mice as the infection occurred at a similar rate in both the operative and non-operative legs. X-ray imaging revealed that the operative legs of younger mice had decreased femoral length, likely due to the surgical and/or infectious insult to the more active physis, which was not observed in older mice. Both age groups demonstrated substantial reactive bone changes in the operative leg due to infection. Conclusions The presence of an implant was an important determinant for developing a hematogenous orthopaedic infection in older but not younger mice, whereas younger mice had a similar predilection for developing periarticular infection whether or not an implant was present. On a clinical scale, diagnosing HOIAI may be difficult particularly in at-risk patients with limited examination or other data points. Understanding the influence of age on developing HOIAI may guide clinical surveillance and decision-making in at-risk patients.

scholarly journals Development of an anti-infective coating on the surface of intraosseous implants responsive to enzymes and bacteria

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Xin Liao ◽  
Xingfang Yu ◽  
Haiping Yu ◽  
Jiaqi Huang ◽  
Bi Zhang ◽  
...  
Keyword(s):  
Multilayer Films ◽  
Orthopaedic Implant ◽  
Inhibition Rate ◽  
Biofilm Inhibition ◽  
Bacterial Inhibition ◽  
Activity Effect ◽  
Protein Leakage ◽  
K Wires

Abstract Background Bacterial proliferation on the endosseous implants surface presents a new threat to the using of the bone implants. Unfortunately, there is no effective constructed antibacterial coating which is bacterial anti-adhesion substrate-independent or have long-term biofilm inhibition functions. Methods Drug release effect was tested in Chymotrypsin (CMS) solution and S. aureus. We used bacterial inhibition rate assays and protein leakage experiment to analyze the in vitro antibacterial effect of (Montmorillonite/Poly-l-lysine-Chlorhexidine)10 [(MMT/PLL-CHX)10] multilayer film. We used the CCK-8 assay to analyze the effect of (MMT/PLL-CHX)10 multilayer films on the growth and proliferation of rat osteoblasts. Rat orthopaedic implant-related infections model was constructed to test the antimicrobial activity effect of (MMT/PLL-CHX)10 multilayer films in vivo. Results In this study, the (MMT/PLL-CHX)10 multilayer films structure were progressively degraded and showed well concentration-dependent degradation characteristics following incubation with Staphylococcus aureus and CMS solution. Bacterial inhibition rate assays and protein leakage experiment showed high levels of bactericidal activity. While the CCK-8 analysis proved that the (MMT/PLL-CHX)10 multilayer films possess perfect biocompatibility. It is somewhat encouraging that in the in vivo antibacterial tests, the K-wires coated with (MMT/PLL-CHX)10 multilayer films showed lower infections incidence and inflammation than the unmodified group, and all parameters are close to SHAM group. Conclusion (MMT/PLL-CHX)10 multilayer films provides a potential therapeutic method for orthopaedic implant-related infections.

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