High frequency ultrasound speckle flow imaging - comparision with doppler optical coherence tomography (DOCT)

Abstract Background The modified Rodnan skin score (mRSS) is the current gold standard for skin assessment in systemic sclerosis (SSc) both in clinical trials and practice. Several studies have reported that skin high frequency ultrasound (HFUS) and, more recently, optical coherence tomography (OCT) are able to reflect the severity of the skin disease in SSc. Aim of the study was to compare OCT and HFUS in the assessment of skin involvement in SSc. Methods Dorsal forearm skin of consecutive diffuse cutaneous SSc (dcSSc) patients and matched-healthy controls (HC) were scanned using OCT and HFUS by investigators blinded to the clinical details using Vivosight scanner (1 assessor) and Esaote MyLab70 equipped with a 22 MHZ probe (2 assessors) respectively. Minimum Optical Density (MinOD), Maximum OD (MaxOD) and OD at 300 micron-depth (OD300) (OCT) and skin thickness (HFUS) were measured. Clinical involvement was assessed by a blinded operator using the mRSS and results were cross matched with imaging data. Statistical analysis was performed using GraphPad Prism software V.7.0. Results A total of 88 OCT images and 176 HFUS images were obtained from 22 dcSSc patients [20 Female, mean age 49 (±11) years, 12 with < 5 years disease duration) and 22 HC (20 Female, mean age 50.7 (±6.7) years]. All OCT measures (MinOD, MaxOD and OD300) were significantly lower in SSc patients than in HC (p = 0.011, p < 0.0001, p < 0.0001 respectively). HFUS showed a lower performance in discriminating SSc skin vs HC compared to OCT (overall AUC 0.6 vs 0.72, 0.8 and 0.89 for MinOD, MaxOD and OD300 respectively). Nevertheless, mean HFUS skin thickness significantly correlated with mRSS at site of analysis (r = 0.47, p = 0.0013) and showed overall excellent interobserver reliability between assessors (ICC >0.8). Importantly, MaxOD and OD300 negatively correlated with HFUS skin thickness (r=-0.32, p = 0.035; r=-0.31, p = 0.039). Conclusion OCT of the skin has been previously validated against skin biopsy in SSc. Our results validate HFUS against OCT and indicate that HFUS of the skin is a reliable measure of skin involvement. Further, here we show that HFUS and OCT outperform each other in measuring different aspects of skin involvement in SSc and they offer complementary surrogate outcome measures of disease. Disclosures D. Temiz Karadag None. G. Lettieri None. V. Picerno None. O. Gundogdu None. M. Padula None. G.A. Mennillo None. A.A. Padula None. F. Del Galdo None. S. D'Angelo None. G. Abignano None.

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Comparison of optical coherence tomography and high frequency ultrasound imaging in mice for the assessment of skin morphology and intradermal volumes

Scientific Reports ◽

10.1038/s41598-019-50104-4 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 1

Author(s):

Kornelia Schuetzenberger ◽

Martin Pfister ◽

Alina Messner ◽

Vanessa Froehlich ◽

Gerhard Garhoefer ◽

...

Keyword(s):

Optical Coherence Tomography ◽

High Frequency ◽

Ex Vivo ◽

Clinical Settings ◽

Optical Coherence ◽

High Frequency Ultrasound ◽

Skin Morphology ◽

Frequency Ultrasound ◽

Good Agreement

Abstract Optical coherence tomography (OCT) and high-frequency ultrasound (HFUS), two established imaging modalities in the field of dermatology, were evaluated and compared regarding their applicability for visualization of skin tissue morphology and quantification of murine intradermal structures. The accuracy and reproducibility of both methods were assessed ex vivo and in vivo using a standardized model for intradermal volumes based on injected soft tissue fillers. OCT revealed greater detail in skin morphology, allowing for detection of single layers due to the superior resolution. Volumetric data measured by OCT (7.9 ± 0.3 μl) and HFUS (7.7 ± 0.5 μl) were in good agreement and revealed a high accuracy when compared to the injected volume of 7.98 ± 0.8 µl. In vivo, OCT provided a higher precision (relative SD: 26% OCT vs. 42% HFUS) for the quantification of intradermal structures, whereas HFUS offered increased penetration depth enabling the visualization of deeper structures. A combination of both imaging technologies might be valuable for tumor assessments or other dermal pathologies in clinical settings.

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High-resolution imaging diagnosis and staging of bladder cancer: comparison between optical coherence tomography and high-frequency ultrasound

Journal of Biomedical Optics ◽

10.1117/1.2978059 ◽

2008 ◽

Vol 13 (5) ◽

pp. 054007 ◽

Cited By ~ 16

Author(s):

Zhijia Yuan ◽

Zhenguo Wang ◽

Rubin Pan ◽

Jingxuan Liu ◽

Harris Cohen ◽

...

Keyword(s):

Bladder Cancer ◽

Optical Coherence Tomography ◽

High Resolution ◽

High Frequency ◽

High Resolution Imaging ◽

Optical Coherence ◽

Imaging Diagnosis ◽

High Frequency Ultrasound ◽

Resolution Imaging ◽

Frequency Ultrasound

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Photoacoustic/Ultrasound/Optical Coherence Tomography Evaluation of Melanoma Lesion and Healthy Skin in a Swine Model

Sensors ◽

10.3390/s19122815 ◽

2019 ◽

Vol 19 (12) ◽

pp. 2815 ◽

Cited By ~ 8

Author(s):

Karl Kratkiewicz ◽

Rayyan Manwar ◽

Ali Rajabi-Estarabadi ◽

Joseph Fakhoury ◽

Jurgita Meiliute ◽

...

Keyword(s):

Optical Coherence Tomography ◽

High Frequency ◽

Photoacoustic Imaging ◽

Diagnostic Methods ◽

Swine Model ◽

Optical Coherence ◽

Healthy Skin ◽

High Frequency Ultrasound ◽

Rapid Drop ◽

Frequency Ultrasound

The marked increase in the incidence of melanoma coupled with the rapid drop in the survival rate after metastasis has promoted the investigation into improved diagnostic methods for melanoma. High-frequency ultrasound (US), optical coherence tomography (OCT), and photoacoustic imaging (PAI) are three potential modalities that can assist a dermatologist by providing extra information beyond dermoscopic features. In this study, we imaged a swine model with spontaneous melanoma using these modalities and compared the images with images of nearby healthy skin. Histology images were used for validation.

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