Immunologic Mechanisms in the Induction and Regression of Shope Papilloma Virus-Induced Epidermal Papillomas of Rats

A method has been devised to determine the location of infective Shope virus in the papillomas of cottontail rabbits. Frozen sections of the growths were burned selectively with a microcautery to destroy either the keratinized or proliferating layer and the sections were then applied directly to the sensitized epidermis of domestic rabbits. No papillomas appeared when the keratohyaline and keratinized areas had been eliminated leaving the proliferating cell layer, whereas papillomas arose when the proliferating cell areas were destroyed leaving the keratohyaline and keratinized layers. The results indicate that infective Shope papilloma virus is situated mainly, perhaps entirely, in the keratohyaline and keratinized areas of cottontail papillomas. This is in accord with the previous disclosure by the fluorescence technique that virus antigen in demonstrable quantity is present only in these situations.

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Purification and chemical analysis of Shope papilloma virus

Virology ◽

10.1016/0042-6822(65)90106-6 ◽

1965 ◽

Vol 27 (3) ◽

pp. 273-281 ◽

Cited By ~ 13

Author(s):

S.J. Kass ◽

C.A. Knight

Keyword(s):

Chemical Analysis ◽

Papilloma Virus ◽

Shope Papilloma Virus

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Shope Papilloma Virus: A Passenger in Man and its Significance to the Potential Control of the Host Genome

Nature ◽

10.1038/2121220a0 ◽

1966 ◽

Vol 212 (5067) ◽

pp. 1220-1222 ◽

Cited By ~ 22

Author(s):

STANFIELD ROGERS

Keyword(s):

Host Genome ◽

Papilloma Virus ◽

Potential Control ◽

Shope Papilloma Virus

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Evidence for and Localization of Vegetative Viral DNA Replication by Autoradiographic Detection of RNA{middle dot}DNA Hybrids in Sections of Tumors Induced by Shope Papilloma Virus

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.68.8.1876 ◽

1971 ◽

Vol 68 (8) ◽

pp. 1876-1880 ◽

Cited By ~ 42

Author(s):

G. Orth ◽

P. Jeanteur ◽

O. Croissant

Keyword(s):

Dna Replication ◽

Viral Dna ◽

Viral Dna Replication ◽

Papilloma Virus ◽

Shope Papilloma Virus ◽

Autoradiographic Detection

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FLUORESCENT ANTIBODY DETECTION OF THE ANTIGENS OF THE SHOPE PAPILLOMA VIRUS IN PAPILLOMAS OF THE WILD AND DOMESTIC RABBIT

Journal of Experimental Medicine ◽

10.1084/jem.106.4.555 ◽

1957 ◽

Vol 106 (4) ◽

pp. 555-562 ◽

Cited By ~ 69

Author(s):

Wilbur Fiske Noyes ◽

Robert C. Mellors

Keyword(s):

Nucleic Acid ◽

Early Stage ◽

Fluorescent Antibody ◽

Antibody Detection ◽

Wild Rabbit ◽

Papilloma Virus ◽

Viral Antigens ◽

Stage Of Development ◽

Domestic Rabbit ◽

Shope Papilloma Virus

The results obtained by a fluorescent antibody study of the Shope papilloma virus in papillomas of the wild and domestic rabbit are presented. In the wild rabbit papillomas the viral antigens occurred exclusively in the nucleus and were present in the differentiating cells of the keratohyaline layers and in the keratinized layers. The antigens were not present in the deeper proliferating epithelial cells of the papillomas. The Shope viral antigens were present in very minute amounts in papillomas of the domestic rabbit, as compared with papillomas of the wild rabbit, and were only detected in the superficial keratinized layers. It is postulated that virus is present in the nuclei of the proliferating cells of the papillomas of the wild and domestic rabbit but exists there in an early stage of development, consisting mainly of nucleic acid and deficient in protein, therefore non-antigenic and not demonstrable by fluorescent antibody. The nucleic acid moiety of the virus may be infective, and the protein component may provide immunologic specificity and serve to preserve transmissibility. The protein-deficient virus can be referred to as masked virus.

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INDUCTION OF TUMORS IN DOMESTIC RABBITS WITH NUCLEIC ACID PREPARATIONS FROM PARTIALLY PURIFIED SHOPE PAPILLOMA VIRUS AND FROM EXTRACTS OF THE PAPILLOMAS OF DOMESTIC AND COTTONTAIL RABBITS

Journal of Experimental Medicine ◽

10.1084/jem.114.4.485 ◽

1961 ◽

Vol 114 (4) ◽

pp. 485-500 ◽

Cited By ~ 59

Author(s):

Yohei Ito ◽

Charles A. Evans

Keyword(s):

Deoxyribonucleic Acid ◽

Virus Preparation ◽

Minute Amount ◽

Papilloma Virus ◽

Domestic Rabbit ◽

Intact Virus ◽

Negative Findings ◽

Domestic Rabbits ◽

Tumorigenic Activity ◽

Shope Papilloma Virus

A deoxyribonucleic acid preparation which showed infectivity and tumorigenic activity in domestic rabbits was isolated from the papillomatous tissue of wild cottontail rabbits by phenolic deproteinization procedure. The activity of the preparation could be completely abolished by its exposure to a minute amount (0.02 µg/ml) of DNAase. Antisera against Shope papilloma virus did not block the tumorigenic activity of the preparation, and trypsin and chymotrypsin had no effect on it. The extraction with phenol of a partially purified virus preparation also yielded extracts with tumorigenic potency. Extracts obtained from the domestic rabbit papilloma and submitted to phenolic deproteinization also proved infective and tumorigenic in rabbits of this sort, although the level of "tumorigenicity" was much lower than that of the cottontail preparations. Tests for intact virus, carried out with half of the extracts yielded wholly negative findings.

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