Aqueous Fluid as a Viable Substitute for Vitreous Fluid in Postmortem Chemistry Analysis

2019 ◽  
Vol 65 (2) ◽  
pp. 508-512 ◽  
Author(s):  
Daniel C. Butler ◽  
Cynthia Schandl ◽  
Madison Hyer ◽  
Susan Erin Presnell
2019 ◽  
Vol 57 (4) ◽  
pp. 409-418 ◽  
Author(s):  
ShuZe Chen ◽  
HuiMin Zhong ◽  
Yan Wang ◽  
ZiHong Wang ◽  
XiaoQian Liang ◽  
...  

Abstract Aim To analyse the expression of lncRNA-ANRIL and other related factors in different human body fluids, explore the clinical significance of ANRIL and validate whether ANRIL is interrelated with the renin–angiotensin system and NF-κB signalling pathway. Methods Ninety-one patients were included in this cross-sectional study and were divided into the NDM group (20 patients), DM group (25 patients), NPDR group (21 patients) and PDR group (25 patients). Basic information and samples of serum, aqueous fluid and vitreous fluid were collected before vitrectomy or intravitreal injection. The transcription and levels of ANRIL and other related factors were detected by RT-PCR and ELISA. Statistical Package for Social Sciences software was used for statistical analysis. Results ANRIL expression varied among different groups and body fluids. There was no difference in ANRIL expression between the NDM and DM groups, but the level of ANRIL was significantly lower in the DM group than in the NPDR and PDR group. In vitreous fluid, ANRIL expression was positively correlated with Ang II, p65 and VEGF expression in the PDR group. The expression of ANRIL in serum was not significantly correlated with age or the random blood sugar but was positively correlated with diabetic duration and HbAc1 level. Conclusions Levels of lncRNA-ANRIL are higher in DR patient and correlated with the progression of DR that may be used as an indicator to predict the development of DR. The activation of the RAS and the NF-κB pathway may be closely related to the upregulation of ANRIL. Clinical trial number ChiCTR1800017500. Registry Chinese Clinical Trial Registry.


2021 ◽  
Vol 10 (11) ◽  
pp. 2309
Author(s):  
Sarah R. Weber ◽  
Yuanjun Zhao ◽  
Christopher Gates ◽  
Jingqun Ma ◽  
Felipe da Veiga Leprevost ◽  
...  

Vitreous fluid is becoming an increasingly popular medium for the study of retinal disease. Numerous studies have demonstrated that proteomic analysis of the vitreous from patients with proliferative diabetic retinopathy yields valuable molecular information regarding known and novel proteins and pathways involved in this disease. However, there is no standardized methodology for vitreous proteomic studies. Here, we share a suggested protocol for such studies and outline the various experimental and analytic methods that are currently available. We also review prior mass spectrometry-based proteomic studies of the vitreous from patients with proliferative diabetic retinopathy, discuss common pitfalls of these studies, and propose next steps for moving the field forward.


2012 ◽  
Vol 109 (46) ◽  
pp. 18695-18700 ◽  
Author(s):  
T. Kawamoto ◽  
M. Kanzaki ◽  
K. Mibe ◽  
K. N. Matsukage ◽  
S. Ono

2010 ◽  
Vol 62 (4) ◽  
pp. 915-919 ◽  
Author(s):  
C. Bayer ◽  
M. Follmann ◽  
T. Melin ◽  
T. Wintgens ◽  
K. Larsson ◽  
...  

Many phenolic compounds show high boiling points, low molecular weights, moderate polarities or high toxicities. Therefore, conventional wastewater treatment is limited or expensive. Recycling of the separated compounds is often not possible. But, if liquid-liquid reactive extraction is linked to a non-porous membrane, some or all of the above mentioned limitations may be overcome. The key element is a composite membrane with a dense, hydrophobic top layer which avoids the mixing of the two aqueous fluid streams. The dilute phenol stream is one of them, the other is caustic soda as stripping solvent. Since the basics of this technology have been discussed before, the scope of this study is to facilitate process implementation and integration. To this end, a life cycle assessment framework is used to identify the optimal equipment size for the treatment of wastewater that may, for example, originate from the production of polycarbonate. Limiting for this application is not the environmental performance though, but most likely process economics.


2021 ◽  
Author(s):  
Zhihua Wang ◽  
Daniel Newton ◽  
Aqib Qureshi ◽  
Yoshito Uchiyama ◽  
Georgina Corona ◽  
...  

Abstract This Extended Reach Drilling (ERD) field re-development of a giant offshore field in the United Arab Emirates (UAE) requires in most cases extremely long laterals to reach the defined reservoir targets. However, certain areas of the field show permeability and / or pressure variations along the horizontal laterals. This heterogeneity requires an inflow control device (ICD) lower completion liner to deliver the required well performance that will adequately produce and sweep the reservoir. The ICD lower completion along with the extremely long laterals means significant time is spent switching the well from reservoir drilling fluid (RDF) non-aqueous fluid (NAF) to an aqueous completion brine. To reduce the amount of rig time spent on the displacement portion of the completion phase, an innovative technology was developed to enable the ICDs to be run in hole in a closed position and enable circulating through the end of the liner. The technology uses a dissolvable material, which is installed in the ICD to temporarily plug it. The dissolvable material is inert to the RDF NAF while the ICDs are run into hole, and then dissolves in brine after the well is displaced from RDF NAF to completion brine, changing the ICDs from closed to an open position. The ability to circulate through the end of the liner, with the support of the plugged ICDs, when the lower completion is deployed and at total depth (TD), enables switching the well from RDF NAF drilling fluid to an aqueous completion brine without the associated rig time of the original displacement method. The technique eliminates the use of a dedicated inner displacement string and allows for the displacement to be performed with the liner running string, saving 4-5 days per well. An added bonus is that the unique design allowed for this feature to be retrofitted to existing standard ICDs providing improved inventory control. In this paper the authors will demonstrate the technology and system developed to perform this operation, as well as the qualification testing, field installations, and lessons learned that were required to take this solution from concept to successful performance improvement initiative.


2003 ◽  
Vol 153 (2) ◽  
pp. 289-304 ◽  
Author(s):  
Shenghui Li ◽  
Martyn J. Unsworth ◽  
John R. Booker ◽  
Wenbo Wei ◽  
Handong Tan ◽  
...  

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