Error factors of AC-DC conversion circuits accuracy

Voltage standard source is an important basic instrument in the electrical measurement and testing field, and it has important guarantee significance for many industries. AC voltage standard source is based on AC-DC conversion technology to achieve output function of high precision AC voltage. In order to get a better effect of AC-DC conversion, the three factors—operational amplifier, resistance and diode—that have a greater impact on the accuracy of the rectifier output were taken the research object, and their impacts on the circuit error were analyzed respectively. The output result was compared with the output of full wave precision rectification circuit under ideal state, and the scheme of reducing error in the precision rectifier circuit was summarized, which provides a reference for improving the accuracy of AC-DC conversion in the design of full wave rectifier circuit.

Innovative Washpipe Free Circulating ICD Delivers Reduced Well Construction Costs

This Extended Reach Drilling (ERD) field re-development of a giant offshore field in the United Arab Emirates (UAE) requires in most cases extremely long laterals to reach the defined reservoir targets. However, certain areas of the field show permeability and / or pressure variations along the horizontal laterals. This heterogeneity requires an inflow control device (ICD) lower completion liner to deliver the required well performance that will adequately produce and sweep the reservoir. The ICD lower completion along with the extremely long laterals means significant time is spent switching the well from reservoir drilling fluid (RDF) non-aqueous fluid (NAF) to an aqueous completion brine. To reduce the amount of rig time spent on the displacement portion of the completion phase, an innovative technology was developed to enable the ICDs to be run in hole in a closed position and enable circulating through the end of the liner. The technology uses a dissolvable material, which is installed in the ICD to temporarily plug it. The dissolvable material is inert to the RDF NAF while the ICDs are run into hole, and then dissolves in brine after the well is displaced from RDF NAF to completion brine, changing the ICDs from closed to an open position. The ability to circulate through the end of the liner, with the support of the plugged ICDs, when the lower completion is deployed and at total depth (TD), enables switching the well from RDF NAF drilling fluid to an aqueous completion brine without the associated rig time of the original displacement method. The technique eliminates the use of a dedicated inner displacement string and allows for the displacement to be performed with the liner running string, saving 4-5 days per well. An added bonus is that the unique design allowed for this feature to be retrofitted to existing standard ICDs providing improved inventory control. In this paper the authors will demonstrate the technology and system developed to perform this operation, as well as the qualification testing, field installations, and lessons learned that were required to take this solution from concept to successful performance improvement initiative.

Whole genome sequencing of colonies derived from cannabis flowers and the impact of media selection on benchmarking total yeast and mold detection tools

Background: Cannabis products are subjected to microbial testing for human pathogenic fungi and bacteria. These testing requirements often rely on non-specific colony forming unit (CFU/g) specifications without clarity on which medium, selection or growth times are required. We performed whole genome sequencing to assess the specificity of colony forming units (CFU) derived from three different plating media: Potato Dextrose Agar (PDA), PDA with chloramphenicol and Dichloran Rose Bengal with chloramphenicol (DRBC). Methods: Colonies were isolated from each medium type and their whole genomes sequenced to identify the diversity of microbes present on each medium selection. Fungal Internal Transcribed Spacer (ITS3) and Bacterial 16S RNA(16S) quantitative polymerase chain reactions (qPCR) were performed, to correlate these CFUs with fungi- and bacterial- specific qPCR. Results: Each plating medium displayed a ten-fold difference in CFU counts. PDA with chloramphenicol showed the highest diversity and the highest concordance with whole genome sequencing. According to ITS3 and 16S qPCR confirmed with whole genome sequencing, DRBC under counted yeast and mold while PDA without chloramphenicol over counted CFUs due to bacterial growth without selection. Conclusions: Colony Forming Unit regulations lack specificity. Each medium produces significant differences in CFU counts. These are further dependent on subjective interpretation, failure to culture most microbes, and poor selection between bacteria and fungi. Given the most human pathogenic microbes found on cannabis are endophytes which culture fails to detect, molecular methods offer a solution to this long-standing quantification problem in the cannabis testing field.

Whole genome sequencing of colonies derived from cannabis flowers and the impact of media selection on benchmarking total yeast and mold detection tools

Background: Cannabis products are subjected to microbial testing for pathogenic fungi and bacteria. These testing requirements often rely on non-specific colony forming unit (CFU/g) specifications without clarity on which medium, selection or growth times are required. We performed whole genome sequencing to assess the specificity of colony forming units (CFU) derived from three different plating media: Potato Dextrose Agar (PDA), PDA with chloramphenicol and Dichloran Rose Bengal with chloramphenicol (DRBC). Methods: Colonies were isolated from each medium type and their whole genomes sequenced to identify the diversity of microbes present on each medium selection. Fungal Internal Transcribed Spacer (ITS3) and Bacterial 16S RNA(16S) quantitative polymerase chain reactions (qPCR) were performed, to correlate these CFUs with fungi- and bacterial- specific qPCR. Results: Each plating medium displayed a ten-fold difference in CFU counts. PDA with chloramphenicol showed the highest diversity and the highest concordance with whole genome sequencing. According to ITS3 and 16S qPCR confirmed with whole genome sequencing, DRBC under counted yeast and mold while PDA without chloramphenicol over counted CFUs due to bacterial growth without selection. Conclusions: Colony Forming Unit regulations lack specificity. Each medium produces significant differences in CFU counts. These are further dependent on subjective interpretation, failure to culture most microbes, and poor selection between bacteria and fungi. Given the most pathogenic microbes found on cannabis are endophytes which culture fails to detect, molecular methods offer a solution to this long-standing quantification problem in the cannabis testing field.

High-Throughput Sequencing of Small RNAs for the Sanitary Certification of Viruses in Grapevine

Biological indexing is the method generally recognized for the certification of propagative grapevines in many countries, and it is mandatory in the European Union. It consists of the evaluation of the plant material after grafting on indicators that are inspected for symptom development. This is a lengthy process that requires well-trained workers, testing field, etc. Alternative diagnostic methods such as serology and RT-qPCR have been discarded for certification because of their intrinsic drawbacks. In turn, high-throughput sequencing (HTS) of plant RNA has been proposed as a plausible alternative to bioassay, but before it is accepted, different aspects of this process must be evaluated. We have compared the HTS of small RNAs with bioassays and other diagnostic methods from a set of 40 grapevine plants submitted for certification. The results allowed the authors the identification of numerous grapevine viruses in the samples, as well as different variants. Besides, relationships between symptom expression and viromes were investigated, in particular leafroll-associated viruses. We compared HTS results using analytical and bioinformatics approaches in order to define minimum acceptable quality standards for certification schemes, resulting in a pipeline proposal. Finally, the comparison between HTS and bioassay resulted favorable for the former in terms of reliability, cost, and timing.

A Survey of Field-based Testing Techniques

Field testing refers to testing techniques that operate in the field to reveal those faults that escape in-house testing. Field testing techniques are becoming increasingly popular with the growing complexity of contemporary software systems. In this article, we present the first systematic survey of field testing approaches over a body of 80 collected studies, and propose their categorization based on the environment and the system on which field testing is performed. We discuss four research questions addressing how software is tested in the field, what is tested in the field, which are the requirements , and how field tests are managed , and identify many challenging research directions.

Novel Protocol for Acute In Situ Ecotoxicity Test Using Native Crustaceans Applied to Groundwater Ecosystems

Current standardized laboratory test protocols use model species that have limitations to accurately assess native species responses to stressors. We developed and tested a novel acute in situ protocol for testing field-collected organisms. We used Asellus aquaticus and NaCl as a reference toxicant to test for the effects of location (laboratory vs. in situ), medium (synthetic vs. field water), substrate (presence vs. absence), and protocol replicability. We further tested the protocol using groundwater-adapted isopods: Proasellus assaforensis for the effect of location, P. cavaticus of medium and P.lusitanicus of substrate. Our results showed that A.aquaticus’ lethality obtained with the novel acute in situ protocol did not significantly differ from those from laboratory testing. However, laboratory tested P.assaforensis showed a higher sensitivity, suggesting that its acclimation to laboratory conditions might have pernicious effects. A. aquaticus and P. cavaticus showed a higher mortality using synthetic medium in situ and under laboratory conditions, which overestimated the stressor’s effect. Besides, substrate use had no significant effect. The novel acute in situ protocol allows the use of native species under realistic scenarios. It is particularly well adapted for assessing the risk of groundwater ecosystems but it can be applied to a wide range of ecosystems.