Synthesis and characterization of tracers and development of a fluorescence polarization immunoassay for amantadine with high sensitivity in chicken

Enrofloxacin (ENR) is a widely used fluoroquinolone (FQ) antibiotic for antibacterial treatment of edible animal. In this study, a rapid and highly specific fluorescence polarization immunoassay (FPIA) was developed for monitoring ENR residues in animal foods. First, ENR was covalently coupled to bovine serum albumin (BSA) to produce specific polyclonal antibodies (pAbs). Three fluorescein-labeled ENR tracers (A, B, and C) with different spacers were synthesized and compared to obtain higher sensitivity. Tracer C with the longest arm showed the best sensitivity among the three tracers. The developed FPIA method showed an IC50 (50% inhibitory concentration) of 21.49 ng·mL−1 with a dynamic working range (IC20–IC80) of 4.30–107.46 ng·mL−1 and a limit of detection (LOD, IC10) of 1.68 ng·mL−1. The cross-reactivity (CR) of several structurally related compounds was less than 2%. The recoveries of spiked pork liver and chicken samples varied from 91.3% to 112.9%, and the average coefficients of variation were less than 3.83% and 5.13%, respectively. The immunoassay took only 8 min excluding sample pretreatment. This indicated that the established method had high sensitivity, specificity, and the advantages of simplicity. Therefore, the proposed FPIA provided a useful screening method for the rapid detection of ENR residues in pork liver and chicken.

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Synthesis and Characterization of the Perthiatriarylmethyl Radical and Its Dendritic Derivatives with High Sensitivity and Selectivity to Superoxide Radical

Chemistry - A European Journal ◽

10.1002/chem.201801311 ◽

2018 ◽

Vol 24 (27) ◽

pp. 6865-6865 ◽

Cited By ~ 1

Author(s):

Xiaoli Tan ◽

Shanqing Tao ◽

Wenbo Liu ◽

Antal Rockenbauer ◽

Frederick A. Villamena ◽

...

Keyword(s):

Superoxide Radical ◽

High Sensitivity ◽

Synthesis And Characterization ◽

Sensitivity And Selectivity

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Improved strategies for selection and characterization of new monoclonal anti-carbamazepine antibodies during the screening process using feces and fluorescence polarization immunoassay

Analytical Methods ◽

10.1039/c6ay01968d ◽

2016 ◽

Vol 8 (38) ◽

pp. 6883-6894 ◽

Cited By ~ 9

Author(s):

Lidia Oberleitner ◽

Ursula Dahmen-Levison ◽

Leif-Alexander Garbe ◽

Rudolf J. Schneider

Keyword(s):

Fluorescence Polarization ◽

Fluorescence Polarization Immunoassay ◽

Screening Process

Methodological suggestions for the production of monoclonal hapten antibodies.

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New horizons for the synthesis of nanoparticles: Germanium nanoparticles from metastable GeBr-solutions

Main Group Metal Chemistry ◽

10.1515/mgmc-2021-0026 ◽

2021 ◽

Vol 44 (1) ◽

pp. 243-249

Author(s):

Viktoriia Slynchuk ◽

Martin Hodas ◽

Dominik Naglav-Hansen ◽

Frank Schreiber ◽

Andreas Schnepf

Keyword(s):

Surface Functionalization ◽

Maximum Yield ◽

High Sensitivity ◽

Synthesis And Characterization ◽

Narrow Size Distribution ◽

Reaction Route ◽

Synthesis Of Nanoparticles ◽

New Horizons ◽

Germanium Nanoparticles

Abstract We present the synthesis and characterization of halide-terminated colloidal Ge nanoparticles of 2–10 nm with a narrow size distribution, synthesized via a novel reaction route. The nanoparticles are prepared by the disproportionation reaction of metastable Ge(I)X solutions and are obtained in a maximum yield of 79%. Control of the nanoparticle size is achieved by varying the aging time and/or temperature. The halide termination of the nanoparticles is a perfect prerequisite for further surface functionalization but also leads to a high sensitivity of the germanium nanoparticles to water and air.

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Fluorescence Polarization Immunoassay for the Determination of T-2 and HT-2 Toxins and Their Glucosides in Wheat

Toxins ◽

10.3390/toxins11070380 ◽

2019 ◽

Vol 11 (7) ◽

pp. 380 ◽

Cited By ~ 2

Author(s):

Vincenzo Lippolis ◽

Anna C. R. Porricelli ◽

Erminia Mancini ◽

Biancamaria Ciasca ◽

Veronica M. T. Lattanzio ◽

...

Keyword(s):

Fluorescence Polarization ◽

Screening Method ◽

High Sensitivity ◽

Cross Reactivity ◽

Screening Methods ◽

The European Union ◽

Fluorescence Polarization Immunoassay ◽

Intermediate Precision ◽

Relative Standard

T-2 and HT-2 toxins and their main modified forms (T-2 glucoside and HT-2 glucoside) may co-occur in cereals and cereal-based products. A fluorescence polarization immunoassay (FPIA) was developed for the simultaneous determination of T-2 toxin, HT-2 toxin and relevant glucosides, expressed as sum. The developed FPIA, using a HT-2-specific antibody, showed high sensitivity (IC50 = 2.0 ng/mL) and high cross-reactivity (100% for T-2 toxin and 80% for T-2 and HT-2 glucosides). The FPIA has been used to develop two rapid and easy-to-use methods using two different extraction protocols, based on the use of organic (methanol/water, 90:10, v/v) and non-organic (water) solvents, for the determination of these toxins in wheat. The two proposed methods showed analytical performances in terms of sensitivity (LOD 10 µg/kg) recovery (92–97%) and precision (relative standard deviations ≤13%), fulfilling the criteria for acceptability of an analytical method for the quantitative determination of T-2 and HT-2 toxins established by the European Union. Furthermore, the methods were then validated in accordance with the harmonized guidelines for the validation of screening methods included in the Regulation (EU) No. 519/2014. The satisfactory analytical performances, in terms of intermediate precision (≤25%), cut-off level (80 and 96 µg/kg for the two methods) and rate of false positives (<0.1%) confirmed the applicability of the proposed methods as screening method for assessing the content of these toxins in wheat at the EU indicative levels reported for T-2 and HT-2 toxins.

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